| 106 matches found in 36 documents. Search time: 0.234 seconds. |
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Score: 11.00 |
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| Title: Suppression of two tungro viruses in rice by separable traits originating from cultivar Utri Merah .
| | Author: Encabo JR Cabauatan PQ Cabunagan RC Satoh K Lee JH Kwak DY De Leon TB Macalalad RJ Kondoh H Kikuchi S Choi IR | | Journal: Mol Plant Microbe Interact Citation: V : 22 P : 1268-81 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19737100 Accession (PMID): 19737100 | | Abstract: Rice tungro disease ( RTD ) is caused by Rice tungro spherical virus ( RTSV ) and Rice tungro bacilliform virus ( RTBV ) transmitted by green leafhoppers .
Rice cv .
Utri Merah is highly resistant to RTD .
To define the RTD resistance of Utri Merah , near-isogenic lines ( NIL , BC ( 5 ) or BC ( 6 ) ) developed from Utri Merah and susceptible cv .
Taichung Native 1 ( TN1 ) were evaluated for reactions to RTSV and RTBV .
TW16 is an NIL ( BC ( 5 ) ) resistant to RTD .
RTBV was able to infect both TN1 and TW16 but the levels of RTBV were usually significantly lower in TW16 than in TN1 .
Infection of RTSV was confirmed in TN1 by a serological test but not in TW16 .
However , the global gene-expression pattern in an RTSV-resistant NIL ( BC ( 6 ) ) , TW16-69 , inoculated with RTSV indicated that RTSV can also infect the resistant NIL .
Infection of RTSV in TW16 was later confirmed by reverse-transcription polymerase chain reaction but the level of RTSV was considerably lower in TW16 than in TN1 .
Examination for virus accumulation in another NIL ( BC ( 6 ) ) , TW16-1029 , indicated that all plants of TW16-1029 were resistant to RTSV , whereas the resistance to RTBV and symptom severity were segregating among the individual plants of TW16-1029 .
Collectively , these results suggest that RTD resistance of Utri Merah involves suppression of interacting RTSV and RTBV but the suppression trait for RTSV and for RTBV is inherited separately .
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[ Sen. 9, subscore: 3.00 ]: However , the global gene-expression pattern in an RTSV-resistant NIL ( BC ( 6 ) ) , TW16-69 , inoculated with RTSV indicated that RTSV can also infect the resistant NIL .
[ Sen. 10, subscore: 2.00 ]: Infection of RTSV in TW16 was later confirmed by reverse-transcription polymerase chain reaction but the level of RTSV was considerably lower in TW16 than in TN1 .
[ Sen. 12, subscore: 2.00 ]: Collectively , these results suggest that RTD resistance of Utri Merah involves suppression of interacting RTSV and RTBV but the suppression trait for RTSV and for RTBV is inherited separately .
[ Sen. 1, subscore: 1.00 ]: Rice tungro disease ( RTD ) is caused by Rice tungro spherical virus ( RTSV ) and Rice tungro bacilliform virus ( RTBV ) transmitted by green leafhoppers .
[ Sen. 5, subscore: 1.00 ]: Taichung Native 1 ( TN1 ) were evaluated for reactions to RTSV and RTBV .
[ Sen. 8, subscore: 1.00 ]: Infection of RTSV was confirmed in TN1 by a serological test but not in TW16 .
[ Sen. 11, subscore: 1.00 ]: Examination for virus accumulation in another NIL ( BC ( 6 ) ) , TW16-1029 , indicated that all plants of TW16-1029 were resistant to RTSV , whereas the resistance to RTBV and symptom severity were segregating among the individual plants of TW16-1029 .
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Score: 10.00 |
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| Title: RTS , a rice anther-specific gene is required for male fertility and its promoter sequence directs it issue-specific gene expression in different plant species .
| | Author: Luo H Lee JY Hu Q Nelson-Vasilchik K Eitas TK Lickwar C Kausch AP Chandlee JM Hodges TK .
| | Journal: Plant Mol . Biol . Citation: V : 62 ( 3 ) P : 397-408 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16897470 Accession (PMID): 16897470 | | Abstract: A tapetum-specific gene , RTS , has been isolated by differential screening of a cDNA library from rice panicles .
RTS is a unique gene in the rice genome .
RNA blot analysis and in situ hybridization indicates that this gene is predominantly expressed in the anthers tapetum during meiosis and disappears before anthesis .
RTS has no introns and encodes a putative polypeptide of 94 amino acids with a hydrophobic N-terminal region .
The nucleotide and deduced amino acid sequence of the gene do not show significant homology to any known sequences .
However , a sequence in the promoter region , GAATTTGTTA , differs only by one or two nucleotides from one of the conserved motifs in the promoter region of two pollen-specific genes of tomato .
Several other sequence motifs found in other anther-specific promoters were also identified in the promoter of the RTS gene .
Transgenic and antisense RNA approaches revealed that RTS gene is required for male fertility in rice .
The promoter region of RTS , when fused to the Bacillus amyloliquefaciens ribonuclease gene , barnase , or the antisense of the RTS gene , is able to drive it issue-specific expression of both genes in rice , creeping bentgrass ( Agrostis stolonifera L ) and Arabidopsis , conferring male sterility to the transgenic plants .
Light and near-infrared confocal microscopy of cross-sections through developing flowers of male-sterile transgenics shows that it issue-specific expression of barnase or the antisense RTS genes interrupts tapetal development , resulting in deformed non-viable pollen .
These results demonstrate a critical role of the RTS gene in pollen development in rice and the versatile application of the RTS gene promoter in directing anther-specific gene expression in both monocotyledonous and dicotyledonous plants , pointing to a potential for exploiting this gene and its promoter for engineering male sterility for hybrid production of various plant species . | Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: The promoter region of RTS , when fused to the Bacillus amyloliquefaciens ribonuclease gene , barnase , or the antisense of the RTS gene , is able to drive it issue-specific expression of both genes in rice , creeping bentgrass ( Agrostis stolonifera L ) and Arabidopsis , conferring male sterility to the transgenic plants .
[ Sen. 11, subscore: 2.00 ]: These results demonstrate a critical role of the RTS gene in pollen development in rice and the versatile application of the RTS gene promoter in directing anther-specific gene expression in both monocotyledonous and dicotyledonous plants , pointing to a potential for exploiting this gene and its promoter for engineering male sterility for hybrid production of various plant species .
[ Sen. 1, subscore: 1.00 ]: A tapetum-specific gene , RTS , has been isolated by differential screening of a cDNA library from rice panicles .
[ Sen. 2, subscore: 1.00 ]: RTS is a unique gene in the rice genome .
[ Sen. 4, subscore: 1.00 ]: RTS has no introns and encodes a putative polypeptide of 94 amino acids with a hydrophobic N-terminal region .
[ Sen. 7, subscore: 1.00 ]: Several other sequence motifs found in other anther-specific promoters were also identified in the promoter of the RTS gene .
[ Sen. 8, subscore: 1.00 ]: Transgenic and antisense RNA approaches revealed that RTS gene is required for male fertility in rice .
[ Sen. 10, subscore: 1.00 ]: Light and near-infrared confocal microscopy of cross-sections through developing flowers of male-sterile transgenics shows that it issue-specific expression of barnase or the antisense RTS genes interrupts tapetal development , resulting in deformed non-viable pollen .
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Score: 9.00 |
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| Title: Single nucleotide polymorphisms in a gene for translation initiation factor ( eIF4G ) of rice ( Oryza sativa ) associated with resistance to Rice tungro spherical virus .
| | Author: Lee JH Muhsin M Atienza GA Kwak DY Kim SM De Leon TB Angeles ER Coloquio E Kondoh H Satoh K Cabunagan RC Cabauatan PQ Kikuchi S Leung H Choi IR | | Journal: Mol Plant Microbe Interact Citation: V : 23 P : 29-38 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19958136 Accession (PMID): 19958136 | | Abstract: Rice tungro disease ( RTD ) is a serious constraint to rice production in South and Southeast Asia .
RTD is caused by Rice tungro spherical virus ( RTSV ) and Rice tungro bacilliform virus .
Rice cv .
Utri Merah is resistant to RTSV .
To identify the gene or genes involved in RTSV resistance , the association of genotypic and phenotypic variations for RTSV resistance was examined in backcross populations derived from Utri Merah and rice germplasm with known RTSV resistance .
Genetic analysis revealed that resistance to RTSV in Utri Merah was controlled by a single recessive gene ( tsv1 ) mapped within an approximately 200-kb region between 22 . 05 and 22 . 25 Mb of chromosome 7 .
A gene for putative translation initiation factor 4G ( eIF4G ( tsv1 ) ) was found in the tsv1 region .
Comparison of eIF4G ( tsv1 ) gene sequences among susceptible and resistant plants suggested the association of RTSV resistance with one of the single nucleotide polymorphism ( SNP ) sites found in exon 9 of the gene .
Examination of the SNP site in the eIF4G ( tsv1 ) gene among various rice plants resistant and susceptible to RTSV corroborated the association of SNP or deletions in codons for Val ( 1060-1061 ) of the predicted eIF4G ( tsv1 ) with RTSV resistance in rice .
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[ Sen. 5, subscore: 3.00 ]: To identify the gene or genes involved in RTSV resistance , the association of genotypic and phenotypic variations for RTSV resistance was examined in backcross populations derived from Utri Merah and rice germplasm with known RTSV resistance .
[ Sen. 9, subscore: 2.00 ]: Examination of the SNP site in the eIF4G ( tsv1 ) gene among various rice plants resistant and susceptible to RTSV corroborated the association of SNP or deletions in codons for Val ( 1060-1061 ) of the predicted eIF4G ( tsv1 ) with RTSV resistance in rice .
[ Sen. 2, subscore: 1.00 ]: RTD is caused by Rice tungro spherical virus ( RTSV ) and Rice tungro bacilliform virus .
[ Sen. 4, subscore: 1.00 ]: Utri Merah is resistant to RTSV .
[ Sen. 6, subscore: 1.00 ]: Genetic analysis revealed that resistance to RTSV in Utri Merah was controlled by a single recessive gene ( tsv1 ) mapped within an approximately 200-kb region between 22 . 05 and 22 . 25 Mb of chromosome 7 .
[ Sen. 8, subscore: 1.00 ]: Comparison of eIF4G ( tsv1 ) gene sequences among susceptible and resistant plants suggested the association of RTSV resistance with one of the single nucleotide polymorphism ( SNP ) sites found in exon 9 of the gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
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| Title: Complete nucleotide sequence of the rice tungro spherical virus genome of the highly virulent strain Vt6 .
| | Author: Isogai M Cabauatan PQ Masuta C Uyeda I Azzam O | | Journal: Virus Genes Citation: V : 20 ( 1 ) P : 79-85 Year: Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10766310 Accession (PMID): 10766310 | | Abstract: The complete nucleotide sequence of rice tungro spherical virus ( RTSV ) strain Vt6 , originally from Mindanao , the Philippines , with higher virulence to resistant rice cultivars , was determined and compared with the published sequence for the Philippine-type strain A ( RTSV-A-Shen ) .
It was reported that RTSV-A was not able to infect a rice resistant cultivar TKM 6 ( 10 ) .
RTSV-Vt6 and RTSV-A-Shen share 90% and 95% homology at nucleotide and amino-acid levels , respectively .
The N-terminal leader sequence of RTSV-Vt6 contained a 39-amino acids-region ( positions 65 to 103 ) which was totally different from that of RTSV-A-Shen ; the difference resulted from frame shifting by nucleotide insertions and deletions .
To confirm the amino-acid sequence differences of the leader polypeptide , the same region was cloned and sequenced using a newly obtained variant of RTSV-type 6 , which had been collected in the field of IRRI , and seven field isolates from Mindanao , the Philippines .
Since all the sequences of the target region are identical to that of the Vt6 leader polypeptide , the sequence difference in the leader region seems not to correlate with the virulence of Vt6 . | Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The complete nucleotide sequence of rice tungro spherical virus ( RTSV ) strain Vt6 , originally from Mindanao , the Philippines , with higher virulence to resistant rice cultivars , was determined and compared with the published sequence for the Philippine-type strain A ( RTSV-A-Shen ) .
[ Sen. 3, subscore: 2.00 ]: RTSV-Vt6 and RTSV-A-Shen share 90% and 95% homology at nucleotide and amino-acid levels , respectively .
[ Sen. 4, subscore: 2.00 ]: The N-terminal leader sequence of RTSV-Vt6 contained a 39-amino acids-region ( positions 65 to 103 ) which was totally different from that of RTSV-A-Shen ; the difference resulted from frame shifting by nucleotide insertions and deletions .
[ Sen. 2, subscore: 1.00 ]: It was reported that RTSV-A was not able to infect a rice resistant cultivar TKM 6 ( 10 ) .
[ Sen. 5, subscore: 1.00 ]: To confirm the amino-acid sequence differences of the leader polypeptide , the same region was cloned and sequenced using a newly obtained variant of RTSV-type 6 , which had been collected in the field of IRRI , and seven field isolates from Mindanao , the Philippines .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
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| Title: Short ORF-dependent ribosome shunting operates in an RNA picorna-like virus and a DNA pararetrovirus that cause rice tungro disease .
| | Author: Pooggin MM Rajeswaran R Schepetilnikov MV Ryabova LA | | Journal: PLoS Pathog Citation: V : 8 P : e1002568 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22396650 Accession (PMID): 22396650 | | Abstract: Rice tungro disease is caused by synergistic interaction of an RNA picorna-like virus Rice tungro spherical virus ( RTSV ) and a DNA pararetrovirus Rice tungro bacilliform virus ( RTBV ) .
It is spread by insects owing to an RTSV-encoded transmission factor .
RTBV has evolved a ribosome shunt mechanism to initiate translation of its pregenomic RNA having a long and highly structured leader .
We found that a long leader of RTSV genomic RNA remarkably resembles the RTBV leader : both contain several short ORFs ( sORFs ) and potentially fold into a large stem-loop structure with the first sORF terminating in front of the stem basal helix .
Using translation assays in rice protoplasts and wheat germ extracts , we show that , like in RTBV , both initiation and proper termination of the first sORF translation in front of the stem are required for shunt-mediated translation of a reporter ORF placed downstream of the RTSV leader .
The base pairing that forms the basal helix is required for shunting , but its sequence can be varied .
Shunt efficiency in RTSV is lower than in RTBV .
But in addition to shunting the RTSV leader sequence allows relatively efficient linear ribosome migration , which also contributes to translation initiation downstream of the leader .
We conclude that RTSV and RTBV have developed a similar , sORF-dependent shunt mechanism possibly to adapt to the host translation system and/or coordinate their life cycles .
Given that sORF-dependent shunting also operates in a pararetrovirus Cauliflower mosaic virus and likely in other pararetroviruses that possess a conserved shunt configuration in their leaders it is tempting to propose that RTSV may have acquired shunt cis-elements from RTBV during their co-existence .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice tungro disease is caused by synergistic interaction of an RNA picorna-like virus Rice tungro spherical virus ( RTSV ) and a DNA pararetrovirus Rice tungro bacilliform virus ( RTBV ) .
[ Sen. 2, subscore: 1.00 ]: It is spread by insects owing to an RTSV-encoded transmission factor .
[ Sen. 4, subscore: 1.00 ]: We found that a long leader of RTSV genomic RNA remarkably resembles the RTBV leader : both contain several short ORFs ( sORFs ) and potentially fold into a large stem-loop structure with the first sORF terminating in front of the stem basal helix .
[ Sen. 5, subscore: 1.00 ]: Using translation assays in rice protoplasts and wheat germ extracts , we show that , like in RTBV , both initiation and proper termination of the first sORF translation in front of the stem are required for shunt-mediated translation of a reporter ORF placed downstream of the RTSV leader .
[ Sen. 7, subscore: 1.00 ]: Shunt efficiency in RTSV is lower than in RTBV .
[ Sen. 8, subscore: 1.00 ]: But in addition to shunting the RTSV leader sequence allows relatively efficient linear ribosome migration , which also contributes to translation initiation downstream of the leader .
[ Sen. 9, subscore: 1.00 ]: We conclude that RTSV and RTBV have developed a similar , sORF-dependent shunt mechanism possibly to adapt to the host translation system and/or coordinate their life cycles .
[ Sen. 10, subscore: 1.00 ]: Given that sORF-dependent shunting also operates in a pararetrovirus Cauliflower mosaic virus and likely in other pararetroviruses that possess a conserved shunt configuration in their leaders it is tempting to propose that RTSV may have acquired shunt cis-elements from RTBV during their co-existence .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
