| 12 matches found in 4 documents. Search time: 0.268 seconds. |
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Score: 4.00 |
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| Title: Single nucleotide polymorphisms and haplotype diversity in rice sucrose synthase 3 .
| | Author: Lestari P Lee G Ham TH Reflinur Woo MO Piao R Jiang W Chu SH Lee J Koh HJ | | Journal: J Hered Citation: V : 102 P : 735-46 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21914668 Accession (PMID): 21914668 | | Abstract: Rice sucrose synthase 3 ( RSUS3 ) is expressed predominantly in rice seed endosperm and is thought to play an important role in starch filling during the milky stage of rice seed ripening .
Because the genetic diversity of this locus is not known yet , the full sequence of RSUS3 from 43 rice varieties was amplified to examine the distribution of DNA polymorphisms .
A total of 254 sequence variants , including SNPs and insertion/deletions , were successfully identified in the 7733 bp sequence that comprises the promoter , exons and introns , and 3 downstream nontranscribed region ( NTR ) .
Eleven haplotypes were distinguished among the 43 rice varieties based on nucleotide variation in the 3 defined regions ( 5 NTR , transcript , and 3 NTR ) .
The promoter region showed evidence of a base change on a cis-element that might influence the functional role of the motif in seed-specific expression .
The genetic diversity of the RSUS3 gene sequences in the rice germplasm used in this study appears to be the result of nonrandom processes .
Analysis of polymorphism sites indicated that at least 11 recombinations have occurred , primarily in the transcribed region .
This finding provides insight into the development of a cladistic approach for establishing future genetic association studies of the RSUS3 locus .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice sucrose synthase 3 ( RSUS3 ) is expressed predominantly in rice seed endosperm and is thought to play an important role in starch filling during the milky stage of rice seed ripening .
[ Sen. 2, subscore: 1.00 ]: Because the genetic diversity of this locus is not known yet , the full sequence of RSUS3 from 43 rice varieties was amplified to examine the distribution of DNA polymorphisms .
[ Sen. 6, subscore: 1.00 ]: The genetic diversity of the RSUS3 gene sequences in the rice germplasm used in this study appears to be the result of nonrandom processes .
[ Sen. 8, subscore: 1.00 ]: This finding provides insight into the development of a cladistic approach for establishing future genetic association studies of the RSUS3 locus .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Differentially and developmentally regulated expression of three rice sucrose synthase genes .
| | Author: Wang AY Kao MH Yang WH Sayion Y Liu LF Lee PD Su JC .
| | Journal: Plant Cell Physiol .
Citation: V : 40 ( 8 ) P : 800-7 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10555303 Accession (PMID): 10555303 | | Abstract: The spatial and temporal distribution of sucrose synthase ( RSuS ) in rice ( Oryza sativa L ) was studied by Western and immunohistochemical analyses using the monospecific antibodies for three RSuS isoforms .
In leaf it issues , RSuS1 was localized in the mesophyll while RSuS2 was in the phloem in addition to the mesophyll .
In the roots , only RSuS1 was found in the phloem .
No RSuS3 could be detected in any parts of etiolated seedlings .
The expression of each RSus gene is closely linked to the seed development .
RSuS1 was present in the aleurone layers of developing seeds , and at a low level in endosperm cells .
RSuS2 was evenly distributed in seed it issues other than the endosperm .
RSuS3 was localized predominantly in the endosperm cells .
The it issue specific localizations of the three gene products suggest that RSuS1 plays a role in sugar transport into endosperm cells where the reaction catalyzed by RSuS3 provides the precursor of starch synthesis .
RSus2 , which is ubiquitously expressed , may play a housekeeping role . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: No RSuS3 could be detected in any parts of etiolated seedlings .
[ Sen. 8, subscore: 1.00 ]: RSuS3 was localized predominantly in the endosperm cells .
[ Sen. 9, subscore: 1.00 ]: The it issue specific localizations of the three gene products suggest that RSuS1 plays a role in sugar transport into endosperm cells where the reaction catalyzed by RSuS3 provides the precursor of starch synthesis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed it issue .
| | Author: Rasmussen TB Donaldson IA .
| | Journal: Plant Cell Rep .
Citation: V : 25 ( 10 ) P : 1035-42 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16670901 Accession (PMID): 16670901 | | Abstract: We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice ( Oryza sativa ) .
The promoter was characterized by deletion analysis and transient expression in guar ( Cyamopsis tetragonoloba ) seed-tissue .
Transient expression was monitored by histochemical GUS assay , and quantitative dual reporter assays comprising firefly luciferase as a test reporter , and Renilla luciferase and GUS as reference reporters .
These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm .
Specificity for this it issue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons .
Removal of a putative intron region only slightly raised the expression level , whereas duplication of the minimal promoter region , in a tandem-repeat rsus3 promoter construct , retained endosperm specificity in guar , and displayed three times the reporter activity observed with the single copy construct . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice ( Oryza sativa ) .
[ Sen. 4, subscore: 1.00 ]: These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm .
[ Sen. 6, subscore: 1.00 ]: Removal of a putative intron region only slightly raised the expression level , whereas duplication of the minimal promoter region , in a tandem-repeat rsus3 promoter construct , retained endosperm specificity in guar , and displayed three times the reporter activity observed with the single copy construct .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Complete structures of three rice sucrose synthase isogenes and differential regulation of their expressions .
| | Author: Huang JW Chen JT Yu WP Shyur LF Wang AY Sung HY Lee PD Su JC .
| | Journal: Biosci . Biotechnol . Biochem . Citation: V : 60 ( 2 ) P : 233-9 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9063969 Accession (PMID): 9063969 | | Abstract: By cloning and sequencing cDNA and genomic DNA and transcription initiation site mapping , the total structures including at least 1 kb of putative regulatory sequences upstream of the transcription initiation sites of three genes encoding rice sucrose synthase isoprotomers were either newly established or amended .
The third type of SS gene , RSus3 , has not been found in other plants .
The structures of the three genes and the gene products were compared and their evolutionary sequence was proposed .
Specific probes for the three SS mRNAs were developed and used for analyzing their steady state levels at different organs and under some physiological stress conditions .
It appears that RSus2 is a house-keeping gene , RSus3 is highly specific to the grain , and the expression of RSus1 shows a tendency to complement that of RSus3 .
A possible cause of the presence of the third rice SS gene was discussed .
We also reported a novel method to synthesize single-stranded DNA for S1 mapping of a transcription initiation site associated with extended secondary structures . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: It appears that RSus2 is a house-keeping gene , RSus3 is highly specific to the grain , and the expression of RSus1 shows a tendency to complement that of RSus3 .
[ Sen. 2, subscore: 1.00 ]: The third type of SS gene , RSus3 , has not been found in other plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
