Score: 1.00 | Title: Five proteins of Laodelphax striatellus are potentially involved in the interactions between rice stripe virus and vector .
| Author: Li S Xiong R Wang X Zhou Y | Journal: PLoS One Citation: V : 6 P : e26585 Year: 2011 Type: In-Process | Literature: oryza Field: abstract Doc ID: pub22028913 Accession (PMID): 22028913 | Abstract: Rice stripe virus ( RSV ) is the type member of the genus Tenuivirus , which relies on the small brown planthopper ( Laodelphax striatellus Fallen ) for its transmission in a persistent , circulative-propagative manner .
To be transmitted , virus must cross the midgut and salivary glands epithelial barriers in a transcytosis mechanism where vector receptors interact with virions , and as propagative virus , RSV need utilize host components to complete viral propagation in vector cells .
At present , these mechanisms remain unknown .
In this paper , we screened L striatellus proteins , separated by two-dimensional electrophoresis ( 2-DE ) , as potential RSV binding molecules using a virus overlay assay of protein blots .
The results , five L striatellus proteins that bound to purified RSV particles in vitro were resolved and identified using mass spectrometry .
The virus-binding capacities of five proteins were further elucidated in yeast two-hybrid screen ( YTHS ) and virus-binding experiments of expressed proteins .
Among five proteins , the receptor for activated protein kinase C ( RACK ) and glyceraldehyde-3-phosphate dehydrogenase ( GAPDH3 ) did not interact with RSV nucleocapsid protein ( NCP ) in YTHS and in far-Western blot , and three ribosomal proteins ( RPL5 , RPL7a and RPL8 ) had specific interactions with RSV .
In dot immunobinding assay ( DIBA ) , all five proteins were able to bind to RSV particles .
The five proteins potential contributions to the interactions between RSV and L striatellus were discussed .
We proposed that RACK and GAPDH3 might be involved in the epithelial transcytosis of virus particles , and three ribosomal proteins probably played potential crucial roles in the infection and propagation of RSV in vector cells .
| Matching Sentences: [ Sen. 7, subscore: 1.00 ]: Among five proteins , the receptor for activated protein kinase C ( RACK ) and glyceraldehyde-3-phosphate dehydrogenase ( GAPDH3 ) did not interact with RSV nucleocapsid protein ( NCP ) in YTHS and in far-Western blot , and three ribosomal proteins ( RPL5 , RPL7a and RPL8 ) had specific interactions with RSV .
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