| 15 matches found in 5 documents. Search time: 1.484 seconds. |
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Score: 8.00 |
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| Title: Position dependent expression of GL2-type homeobox gene , Roc1 : significance for protoderm differentiation and radial pattern formation in early rice embryogenesis .
| | Author: Ito M Sentoku N Nishimura A Hong SK Sato Y Matsuoka M | | Journal: Plant J Citation: V : 29 ( 4 ) P : 497-507 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11846882 Accession (PMID): 11846882 | | Abstract: In early plant embryogenesis , the determination of cell fate in the protodermal cell layer is considered to be the earliest event in radial pattern formation .
To elucidate the mechanisms of epidermal cell fate determination and radial pattern formation in early rice embryogenesis , we have isolated a GL2-type homeobox gene Roc1 ( Rice outermost cell-specific gene1 ) , which is specifically expressed in the protoderm ( epidermis ) .
In early rice embryogenesis , cell division occurs randomly and the morphologically distinct layer structure of the protoderm can not be observed until the embryo reaches more than 100 microm in length .
Nonetheless , in situ hybridization analyses revealed that specific expression of Roc1 in the outermost cells is established shortly after fertilization , much earlier than protoderm differentiation .
In the regeneration process from callus , the Roc1 gene is also expressed in the outermost cells of callus in advance of it issue and organ differentiation , and occurs independently of whether the cells will differentiate into epidermis in the future or not .
Furthermore , this cell-specific Roc1 expression could be induced flexibly in the newly produced outermost cells when we cut the callus .
These findings suggest that the expression of Roc1 in the outermost cells may be dependent on the positional information of cells in the embryo or callus prior to the cell fate determination of the protoderm ( epidermis ) .
Furthermore , the Roc1 expression is downregulated in the inner cells of ligule , which have previously been determined as protodermal cells , also suggesting that the Roc1 expression is position dependent and that this position dependent Roc1 expression is important also in post-embryonic protoderm ( epidermis ) differentiation . | Matching Sentences:
[ Sen. 8, subscore: 3.00 ]: Furthermore , the Roc1 expression is downregulated in the inner cells of ligule , which have previously been determined as protodermal cells , also suggesting that the Roc1 expression is position dependent and that this position dependent Roc1 expression is important also in post-embryonic protoderm ( epidermis ) differentiation .
[ Sen. 2, subscore: 1.00 ]: To elucidate the mechanisms of epidermal cell fate determination and radial pattern formation in early rice embryogenesis , we have isolated a GL2-type homeobox gene Roc1 ( Rice outermost cell-specific gene1 ) , which is specifically expressed in the protoderm ( epidermis ) .
[ Sen. 4, subscore: 1.00 ]: Nonetheless , in situ hybridization analyses revealed that specific expression of Roc1 in the outermost cells is established shortly after fertilization , much earlier than protoderm differentiation .
[ Sen. 5, subscore: 1.00 ]: In the regeneration process from callus , the Roc1 gene is also expressed in the outermost cells of callus in advance of it issue and organ differentiation , and occurs independently of whether the cells will differentiate into epidermis in the future or not .
[ Sen. 6, subscore: 1.00 ]: Furthermore , this cell-specific Roc1 expression could be induced flexibly in the newly produced outermost cells when we cut the callus .
[ Sen. 7, subscore: 1.00 ]: These findings suggest that the expression of Roc1 in the outermost cells may be dependent on the positional information of cells in the embryo or callus prior to the cell fate determination of the protoderm ( epidermis ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Characterization of the cyclophilin gene family of Arabidopsis thaliana and phylogenetic analysis of known cyclophilin proteins .
| | Author: Chou IT Gasser CS .
| | Journal: Plant Mol . Biol . Citation: V : 35 ( 6 ) P : 873-92 Year: 1997 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9426607 Accession (PMID): 9426607 | | Abstract: We have isolated four members of the Arabidopsis cyclophilin ( CyP ) gene family , designated ROC1 to ROC4 ( rotamase CyP ) .
Deduced peptides of ROC1 , 2 and 3 are 75% to 91% identical to Brassica napus cytosolic CyP , contain no leader peptides and include a conserved seven amino-acid insertion relative to mammalian cytosolic CyPs .
Two other Arabidopsis CyPs , ROC5 ( 43H1 ; ATCYP1 ) and ROC6 ( ATCYP2 ) , share these features .
ROC1 , ROC2 , ROC3 and ROC5 are expressed in all tested organs of light-grown plants .
ROC2 and ROC5 show elevated expression in flowers .
Expression of ROC1 , ROC2 , and ROC3 decreases in darkness and these genes also exhibit small elevations in expression upon wouding .
The five Arabidopsis genes encoding putative cytosolic CyPs ( ROC1 , 2 , 3 , 5 and 6 ) contain no introns .
In contrast , ROC4 , which encodes a chloroplast stromal CyP , is interrupted by six introns .
ROC4 is not expressed in roots , and is strongly induced by light .
Phylogenetic trees of all known CyPs and CyP-related proteins provide evidence of possible horizontal transfer of CyP genes between prokaryotes and eukaryotes and of a possible polyphyletic origin of these proteins within eukaryotes .
These trees also show significant grouping of eukaryotic CyPs on the basis of subcellular localization and structure .
Mitochondrial CyPs are closely related to cytosolic CyPs of the source organism , but endoplasmic reticulum CyPs form separate clades .
Known plant CyPs fall into three clades , one including the majority of higher-plant cytosolic CyPs , one including only ROC2 and a related rice CyP , and one including only chlorplast CyPs .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We have isolated four members of the Arabidopsis cyclophilin ( CyP ) gene family , designated ROC1 to ROC4 ( rotamase CyP ) .
[ Sen. 2, subscore: 1.00 ]: Deduced peptides of ROC1 , 2 and 3 are 75% to 91% identical to Brassica napus cytosolic CyP , contain no leader peptides and include a conserved seven amino-acid insertion relative to mammalian cytosolic CyPs .
[ Sen. 4, subscore: 1.00 ]: ROC1 , ROC2 , ROC3 and ROC5 are expressed in all tested organs of light-grown plants .
[ Sen. 6, subscore: 1.00 ]: Expression of ROC1 , ROC2 , and ROC3 decreases in darkness and these genes also exhibit small elevations in expression upon wouding .
[ Sen. 7, subscore: 1.00 ]: The five Arabidopsis genes encoding putative cytosolic CyPs ( ROC1 , 2 , 3 , 5 and 6 ) contain no introns .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Rice globular embryo 4 ( gle4 ) mutant is defective in radial pattern formation during embryogenesis .
| | Author: Kamiya N Nishimura A Sentoku N Takabe E Nagato Y Kitano H Matsuoka M | | Journal: Plant Cell Physiol .
Citation: V : 44 ( 9 ) P : 875-83 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14519768 Accession (PMID): 14519768 | | Abstract: In higher plants , the main elements of the fundamental body plan , the apical-basal and radial patterns , are established during embryogenesis .
We have isolated several globular embryo ( gle ) mutants of rice that fail to develop any embryonic organs .
We expected that these gle mutants might include mutants defective in their radial pattern formation ability .
We developed two markers specifically staining the L2 and L3 layers ( OsSCR and OsPNH1 , respectively ) and characterized the gle mutants by using these markers in addition to the already developed markers Roc1 ( marker for the L1 layer ) , Ramy1A ( marker of the L1 layer of the epithelium ) , and OSH1 ( marker of the apical region ) .
One of the gle mutants , gle4 , expressed Roc1 and Ramy1A at the normal positions , but other markers exhibited an abnormal expression pattern ; that is , both OsPNH1 and OsSCR were expressed in the central region of the embryo and OSH1 expression was not observed .
Calli from the gle4 epithelium regenerated plants with abnormal morphologies .
These results indicate that the GLE4 gene is involved in radial pattern formation during rice embryogenesis to differentiate the L2 and L3 layers , but is not involved in the establishment of the L1 layer or in the formation of embryonic organs . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: We developed two markers specifically staining the L2 and L3 layers ( OsSCR and OsPNH1 , respectively ) and characterized the gle mutants by using these markers in addition to the already developed markers Roc1 ( marker for the L1 layer ) , Ramy1A ( marker of the L1 layer of the epithelium ) , and OSH1 ( marker of the apical region ) .
[ Sen. 5, subscore: 1.00 ]: One of the gle mutants , gle4 , expressed Roc1 and Ramy1A at the normal positions , but other markers exhibited an abnormal expression pattern ; that is , both OsPNH1 and OsSCR were expressed in the central region of the embryo and OSH1 expression was not observed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: [ Cloning and sequencing of ACC oxidase gene from sugarcane ] | | Author: Wang ZZ Li YR Zhang SZ Lin JF Guo LQ .
| | Journal: Yi Chuan Xue Bao Citation: V : 30 ( 1 ) P : 62-9 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12812078 Accession (PMID): 12812078 | | Abstract: The plant hormone ethylene is not only responsible for the initiation of fruit ripening , senescence and dormancy but also for regulating many other plant developmental processes , such as seed germination , root initiation , growth , floral differentiation , sex differentiation and responding to environment stresses .
One of the rate-limiting steps for ethylene biosynthesizing in plant is catalyzed by 1-aminocyclopropane-1-carboxylate ( ACC ) oxidase .
Understanding of ethylene expressive pattern in plant is an entrance to understand the roles of ethylene on plant .
In this paper , two degenerate oligonucleotide primers were designed , coding for two conservative amino acid regions in ACC oxidase protein family , the sequences of the two primers were TAGAGCTCGATGC [ TA ] TG [ CT ] GA [ GA ] AA [ AC ] TGGGG and CGTCTAGAGCTTC [ GA ] AATCTTGGCTCCTT respectively .
A PCR amplification was performed on sugarcane ( Saccharum L Hybrid cv .
ROC16 ) DNA template , and produced a fragment of 940 bp .
By using the program of BLAST on NCBI GenBank database , the sequence presented a very high match with the ACC oxidase genes from other plants , 63 searched out sequences were all ACC oxidase genes .
After alignment on PCgene program , the identities of the cloned fragment with ACC oxidase genes from rice and bamboo were both reaching about 88% .
So we can concluded that the cloned sequence was a member of ACC oxidase genes fragment from sugarcane .
The sequence has been submitted to the GenBank database , the accession number is AF442821 .
According to the ACC oxidase protein family , a intron of 103 bp was excluded and the sequence coded 279 amino acids , which spanned 88% of the putative whole sequence in length .
Alignment and phylogenetic analysis of the amino acid sequence deduced from this fragment and the ACC oxidase sequences of other plants retrieved from GenBank were carried out by using PCgene program .
The putative amino acid sequence shared a homology of 86% with the ACC oxidases of bamboo and rice , 74 . 6% with banana , 70% with tomato and potato and 68% with melon and carnation , which showed that the homology of sugarcane ACC oxidase with monocot was higher than with dicot .
The results of phylogenetic analysis showed that ACC oxidase from sugarcane and ACC oxidases from rice clustered together firstly , and then came those from banana , ACC oxidases of dicot from potato , tomato , petunia , melon , Arabidopsis thaliana and carnation came subsequently .
It indicated that sugarcane ACC oxidase had a closer phylogenetic affinities to the monocot ACC oxidase sequences than to the dicot ACC oxidases sequences .
The clustering results of ACC oxidase molecules accorded with morphological classification system .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: ROC16 ) DNA template , and produced a fragment of 940 bp .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Position dependent expression of GL2-type homeobox gene , Roc1 : significance for protoderm differentiation and radial pattern formation in early rice embryogenesis .
| | Author: Ito M Sentoku N Nishimura A Hong SK Sato Y Matsuoka M | | Journal: Plant J Citation: V : 29 ( 4 ) P : 497-507 Year: 2002 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub11846882 Accession (PMID): 11846882 | | Abstract: In early plant embryogenesis , the determination of cell fate in the protodermal cell layer is considered to be the earliest event in radial pattern formation .
To elucidate the mechanisms of epidermal cell fate determination and radial pattern formation in early rice embryogenesis , we have isolated a GL2-type homeobox gene Roc1 ( Rice outermost cell-specific gene1 ) , which is specifically expressed in the protoderm ( epidermis ) .
In early rice embryogenesis , cell division occurs randomly and the morphologically distinct layer structure of the protoderm can not be observed until the embryo reaches more than 100 microm in length .
Nonetheless , in situ hybridization analyses revealed that specific expression of Roc1 in the outermost cells is established shortly after fertilization , much earlier than protoderm differentiation .
In the regeneration process from callus , the Roc1 gene is also expressed in the outermost cells of callus in advance of it issue and organ differentiation , and occurs independently of whether the cells will differentiate into epidermis in the future or not .
Furthermore , this cell-specific Roc1 expression could be induced flexibly in the newly produced outermost cells when we cut the callus .
These findings suggest that the expression of Roc1 in the outermost cells may be dependent on the positional information of cells in the embryo or callus prior to the cell fate determination of the protoderm ( epidermis ) .
Furthermore , the Roc1 expression is downregulated in the inner cells of ligule , which have previously been determined as protodermal cells , also suggesting that the Roc1 expression is position dependent and that this position dependent Roc1 expression is important also in post-embryonic protoderm ( epidermis ) differentiation . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Position dependent expression of GL2-type homeobox gene , Roc1 : significance for protoderm differentiation and radial pattern formation in early rice embryogenesis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
