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| Title: Four rice genes encoding cysteine synthase : isolation and differential responses to sulfur , nitrogen and light .
| | Author: Nakamura T Yamaguchi Y Sano H | | Journal: Gene Citation: V : 229 ( 1-2 ) P : 155-61 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10095115 Accession (PMID): 10095115 | | Abstract: Four cDNA clones , rcs1 , rcs2 , rcs3 and rcs4 , encoding cysteine synthase [ O-acetylserine ( thiol ) lyase ] were isolated from rice .
The predicted amino acid sequences contain the conserved PXXSVKDR region characteristic of cysteine synthase , which includes the lysine residue that binds the cofactor , pyridoxal 5-phosphate .
Molecular phylogenic analysis suggests that , whereas rcs1 and rcs3 belong to the cytosolic isoform family , rcs2 and rcs4 form a new family of cysteine synthase .
Transcript accumulation of each gene was examined for organ specificity , and also for response to sulfur , nitrogen and light .
The rcs1 transcript accumulated in all organs examined , and was induced in shoots and roots upon sulfur starvation under non-limiting nitrogen conditions .
The rcs2 transcript accumulated in shoots grown in the light , but disappeared almost completely by dark treatment .
The rcs3 transcript was found more abundantly in roots than in shoots , and was reduced in the dark , as well as under sulfur and nitrogen deprivation .
The rcs4 transcript was scarce in all organs examined .
These observations indicate that cysteine synthase genes encode functionally distinct cysteine synthase isoforms , and that they are coordinately regulated by the availability of sulfur , nitrogen , and light . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Four cDNA clones , rcs1 , rcs2 , rcs3 and rcs4 , encoding cysteine synthase [ O-acetylserine ( thiol ) lyase ] were isolated from rice .
[ Sen. 3, subscore: 1.00 ]: Molecular phylogenic analysis suggests that , whereas rcs1 and rcs3 belong to the cytosolic isoform family , rcs2 and rcs4 form a new family of cysteine synthase .
[ Sen. 7, subscore: 1.00 ]: The rcs3 transcript was found more abundantly in roots than in shoots , and was reduced in the dark , as well as under sulfur and nitrogen deprivation .
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