Score: 7.00 |
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| Title: Isolation and characterization of an anther-specific gene , RA8 , from rice ( Oryza sativa L ) .
| | Author: Jeon JS Chung YY Lee S Yi GH Oh BG An G | | Journal: Plant Mol . Biol . Citation: V : 39 ( 1 ) P : 35-44 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10080707 Accession (PMID): 10080707 | | Abstract: An anther-specific cDNA clone of rice , RA8 , was isolated from an anther cDNA library by differential screening .
RNA blot analysis indicated that the RA8 transcript is present specifically in anthers and the transcript level increased as flowers matured , reaching the highest level in mature flowers .
The RA8 clone contains an open reading frame of 264 amino acid residues with a hydrophobic N-terminal region .
The deduced amino acid sequences did not show significant homology to any known sequences .
Genomic DNA blot analysis showed that RA8 is a single-copy gene .
A genomic clone corresponding to the RA8 cDNA was isolated and its promoter region was fused to the beta-glucuronidase ( GUS ) gene .
Transgenic rice plants exhibited anther-specific expression of the GUS reporter gene .
Histochemical GUS analysis showed that the RA8 promoter was active in the tapetum , endothecium , and connective it issues of anthers .
Experiments showed that expression of the gene starts when microspores are released from tetrads , and it reaches to the maximum level at the late vacuolated-pollen stage .
The RA8 promoter may be useful for controlling gene expression in anthers of cereal plants and for generating male-sterile plants . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: An anther-specific cDNA clone of rice , RA8 , was isolated from an anther cDNA library by differential screening .
[ Sen. 2, subscore: 1.00 ]: RNA blot analysis indicated that the RA8 transcript is present specifically in anthers and the transcript level increased as flowers matured , reaching the highest level in mature flowers .
[ Sen. 3, subscore: 1.00 ]: The RA8 clone contains an open reading frame of 264 amino acid residues with a hydrophobic N-terminal region .
[ Sen. 5, subscore: 1.00 ]: Genomic DNA blot analysis showed that RA8 is a single-copy gene .
[ Sen. 6, subscore: 1.00 ]: A genomic clone corresponding to the RA8 cDNA was isolated and its promoter region was fused to the beta-glucuronidase ( GUS ) gene .
[ Sen. 8, subscore: 1.00 ]: Histochemical GUS analysis showed that the RA8 promoter was active in the tapetum , endothecium , and connective it issues of anthers .
[ Sen. 10, subscore: 1.00 ]: The RA8 promoter may be useful for controlling gene expression in anthers of cereal plants and for generating male-sterile plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Isolation and characterization of an anther-specific gene , RA8 , from rice ( Oryza sativa L ) .
| | Author: Jeon JS Chung YY Lee S Yi GH Oh BG An G | | Journal: Plant Mol . Biol . Citation: V : 39 ( 1 ) P : 35-44 Year: 1999 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub10080707 Accession (PMID): 10080707 | | Abstract: An anther-specific cDNA clone of rice , RA8 , was isolated from an anther cDNA library by differential screening .
RNA blot analysis indicated that the RA8 transcript is present specifically in anthers and the transcript level increased as flowers matured , reaching the highest level in mature flowers .
The RA8 clone contains an open reading frame of 264 amino acid residues with a hydrophobic N-terminal region .
The deduced amino acid sequences did not show significant homology to any known sequences .
Genomic DNA blot analysis showed that RA8 is a single-copy gene .
A genomic clone corresponding to the RA8 cDNA was isolated and its promoter region was fused to the beta-glucuronidase ( GUS ) gene .
Transgenic rice plants exhibited anther-specific expression of the GUS reporter gene .
Histochemical GUS analysis showed that the RA8 promoter was active in the tapetum , endothecium , and connective it issues of anthers .
Experiments showed that expression of the gene starts when microspores are released from tetrads , and it reaches to the maximum level at the late vacuolated-pollen stage .
The RA8 promoter may be useful for controlling gene expression in anthers of cereal plants and for generating male-sterile plants . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Isolation and characterization of an anther-specific gene , RA8 , from rice ( Oryza sativa L ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
