| 37 matches found in 18 documents. Search time: 0.478 seconds. |
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Score: 13.00 |
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| Title: Identification of a new locus , Ptr ( t ) , required for rice blast resistance gene Pi-ta-mediated resistance .
| | Author: Jia Y Martin R | | Journal: Mol Plant Microbe Interact Citation: V : 21 P : 396-403 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18321185 Accession (PMID): 18321185 | | Abstract: Resistance to the blast pathogen Magnaporthe oryzae is proposed to be initiated by physical binding of a putative cytoplasmic receptor encoded by a nucleotide binding site-type resistance gene , Pi-ta , to the processed elicitor encoded by the corresponding avirulence gene AVR-Pita .
Here , we report the identification of a new locus , Ptr ( t ) , that is required for Pi-ta-mediated signal recognition .
A Pi-ta-expressing susceptible mutant was identified using a genetic screen .
Putative mutations at Ptr ( t ) do not alter recognition specificity to another resistance gene , Pi-k ( s ) , in the Pi-ta homozygote , indicating that Ptr ( t ) is more likely specific to Pi-ta-mediated signal recognition .
Genetic crosses of Pi-ta Ptr ( t ) and Pi-ta ptr ( t ) homozygotes suggest that Ptr ( t ) segregates as a single dominant nuclear gene .
A ratio of 1 : 1 ( resistant/susceptible ) of a population of BC1 of Pi-ta Ptr ( t ) with pi-ta ptr ( t ) homozygotes indicates that Pi-ta and Ptr ( t ) are linked and cosegregate .
Genotyping of mutants of pi-ta ptr ( t ) and Pi-ta Ptr ( t ) homozygotes using ten simple sequence repeat markers at the Pi-ta region determined that Pi-ta and Ptr ( t ) are located within a 9-megabase region and are of indica origin .
Identification of Ptr ( t ) is a significant advancement in studying Pi-ta-mediated signal recognition and transduction .
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[ Sen. 5, subscore: 3.00 ]: Genetic crosses of Pi-ta Ptr ( t ) and Pi-ta ptr ( t ) homozygotes suggest that Ptr ( t ) segregates as a single dominant nuclear gene .
[ Sen. 6, subscore: 3.00 ]: A ratio of 1 : 1 ( resistant/susceptible ) of a population of BC1 of Pi-ta Ptr ( t ) with pi-ta ptr ( t ) homozygotes indicates that Pi-ta and Ptr ( t ) are linked and cosegregate .
[ Sen. 7, subscore: 3.00 ]: Genotyping of mutants of pi-ta ptr ( t ) and Pi-ta Ptr ( t ) homozygotes using ten simple sequence repeat markers at the Pi-ta region determined that Pi-ta and Ptr ( t ) are located within a 9-megabase region and are of indica origin .
[ Sen. 4, subscore: 2.00 ]: Putative mutations at Ptr ( t ) do not alter recognition specificity to another resistance gene , Pi-k ( s ) , in the Pi-ta homozygote , indicating that Ptr ( t ) is more likely specific to Pi-ta-mediated signal recognition .
[ Sen. 2, subscore: 1.00 ]: Here , we report the identification of a new locus , Ptr ( t ) , that is required for Pi-ta-mediated signal recognition .
[ Sen. 8, subscore: 1.00 ]: Identification of Ptr ( t ) is a significant advancement in studying Pi-ta-mediated signal recognition and transduction .
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Score: 10.00 |
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| Title: Differential expression patterns of two new primary cell wall-related cellulose synthase cDNAs , PtrCesA6 and PtrCesA7 from aspen trees .
| | Author: Samuga A Joshi CP .
| | Journal: Gene Citation: V : 334 ( ) P : 73-82 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15256257 Accession (PMID): 15256257 | | Abstract: Based on elegant molecular genetic analyses , distinct classes of cellulose synthase ( CesA ) genes have been associated with either primary or secondary cell wall development in Arabidopsis .
Here , we report on cloning of two new CesA cDNAs , PtrCesA6 and PtrCesA7 involved in the primary cell wall development in aspen ( Populus tremuloides ) trees .
Both these distinct cDNAs , isolated from a developing xylem cDNA library , share only 60-67% identities with each other as well as with five other previously known aspen CesA cDNAs .
Interestingly , PtrCESA6 from aspen , a dicot species , shares maximum identity of 81-84% with three CESA isoforms from maize and rice , two monocot species .
On the other hand , PtrCESA7 shares a maximum identity of 86% with AtCESA2 , a primary wall-related CesA member from Arabidopsis , a dicot species .
Gene expression analyses by reverse transcriptase-polymerase chain reactions ( RT-PCRs ) suggested that both these genes are expressed at a low level in all aspen it issues examined but PtrCesA7 is expressed at a higher level than PtrCesA6 .
While corroborating these results , in situ mRNA hybridization studies using three different aspen organs also suggested that PtrCesA6 and PtrCesA7 genes are expressed in all expanding cells depositing primary cell wall but PtrCesA7 is expressed at a higher level than PtrCesA6 .
These differential gene expression profiles suggest that each of these CesAs may be playing a specific role during primary cell wall development in aspen trees .
Isolation of two primary wall related CesA genes from xylem it issues also suggest their importance during xylem development , which is traditionally considered to be enriched in secondary cell wall forming cells of economical significance .
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[ Sen. 7, subscore: 4.00 ]: While corroborating these results , in situ mRNA hybridization studies using three different aspen organs also suggested that PtrCesA6 and PtrCesA7 genes are expressed in all expanding cells depositing primary cell wall but PtrCesA7 is expressed at a higher level than PtrCesA6 .
[ Sen. 2, subscore: 2.00 ]: Here , we report on cloning of two new CesA cDNAs , PtrCesA6 and PtrCesA7 involved in the primary cell wall development in aspen ( Populus tremuloides ) trees .
[ Sen. 6, subscore: 2.00 ]: Gene expression analyses by reverse transcriptase-polymerase chain reactions ( RT-PCRs ) suggested that both these genes are expressed at a low level in all aspen it issues examined but PtrCesA7 is expressed at a higher level than PtrCesA6 .
[ Sen. 4, subscore: 1.00 ]: Interestingly , PtrCESA6 from aspen , a dicot species , shares maximum identity of 81-84% with three CESA isoforms from maize and rice , two monocot species .
[ Sen. 5, subscore: 1.00 ]: On the other hand , PtrCESA7 shares a maximum identity of 86% with AtCESA2 , a primary wall-related CesA member from Arabidopsis , a dicot species .
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Score: 5.00 |
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| Title: A cross-sectional investigation of regional patterns of diet and cardio-metabolic risk in India .
| | Author: Daniel CR Prabhakaran D Kapur K Graubard BI Devasenapathy N Ramakrishnan L George PS Shetty H Ferrucci LM Yurgalevitch S Chatterjee N Reddy KS Rastogi T Gupta PC Mathew A Sinha R | | Journal: Nutr J Citation: V : 10 P : 12 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21276235 Accession (PMID): 21276235 | | Abstract: BACKGROUND : The role of diet in Indias rapidly progressing chronic disease epidemic is unclear ; moreover , diet may vary considerably across North-South regions .
METHODS : The India Health Study was a multicenter study of men and women aged 35-69 , who provided diet , lifestyle , and medical histories , as well as blood pressure , fasting blood , urine , and anthropometric measurements .
In each region ( Delhi , n=824 ; Mumbai , n=743 ; Trivandrum , n=2 , 247 ) , we identified two dietary patterns with factor analysis .
In multiple logistic regression models adjusted for age , gender , education , income , marital status , religion , physical activity , tobacco , alcohol , and total energy intake , we investigated associations between regional dietary patterns and abdominal adiposity , hypertension , diabetes , and dyslipidemia .
RESULTS : Across the regions , more than 80% of the participants met the criteria for abdominal adiposity and 10 to 28% of participants were considered diabetic .
In Delhi , the "fruit and dairy" dietary pattern was positively associated with abdominal adiposity [ highest versus lowest tertile , multivariate-adjusted OR and 95% CI : 2 . 32 ( 1 . 03-5 . 23 ) ; Ptrend=0 . 008 ] and hypertension [ 2 . 20 ( 1 . 47-3 . 31 ) ; Ptrend<0 . 0001 ] .
In Trivandrum , the "pulses and rice" pattern was inversely related to diabetes [ 0 . 70 ( 0 . 51-0 . 95 ) ; Ptrend=0 . 03 ] and the "snacks and sweets" pattern was positively associated with abdominal adiposity [ 2 . 05 ( 1 . 34-3 . 14 ) ; Ptrend=0 . 03 ] .
In Mumbai , the "fruit and vegetable" pattern was inversely associated with hypertension [ 0 . 63 ( 0 . 40-0 . 99 ) ; Ptrend=0 . 05 ] and the "snack and meat" pattern appeared to be positively associated with abdominal adiposity .
CONCLUSIONS : Cardio-metabolic risk factors were highly prevalent in this population .
Across all regions , we found little evidence of a Westernized diet ; however , dietary patterns characterized by animal products , fried snacks , or sweets appeared to be positively associated with abdominal adiposity .
Conversely , more traditional diets in the Southern regions were inversely related to diabetes and hypertension .
Continued investigation of diet , as well as other environmental and biological factors , will be needed to better understand the risk profile in this population and potential means of prevention .
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[ Sen. 6, subscore: 2.00 ]: In Delhi , the "fruit and dairy" dietary pattern was positively associated with abdominal adiposity [ highest versus lowest tertile , multivariate-adjusted OR and 95% CI : 2 . 32 ( 1 . 03-5 . 23 ) ; Ptrend=0 . 008 ] and hypertension [ 2 . 20 ( 1 . 47-3 . 31 ) ; Ptrend<0 . 0001 ] .
[ Sen. 7, subscore: 2.00 ]: In Trivandrum , the "pulses and rice" pattern was inversely related to diabetes [ 0 . 70 ( 0 . 51-0 . 95 ) ; Ptrend=0 . 03 ] and the "snacks and sweets" pattern was positively associated with abdominal adiposity [ 2 . 05 ( 1 . 34-3 . 14 ) ; Ptrend=0 . 03 ] .
[ Sen. 8, subscore: 1.00 ]: In Mumbai , the "fruit and vegetable" pattern was inversely associated with hypertension [ 0 . 63 ( 0 . 40-0 . 99 ) ; Ptrend=0 . 05 ] and the "snack and meat" pattern appeared to be positively associated with abdominal adiposity .
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Score: 3.00 |
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| Title: Differential distribution of proteins expressed in companion cells in the sieve element-companion cell complex of rice plants .
| | Author: Fukuda A Fujimaki S Mori T Suzui N Ishiyama K Hayakawa T Yamaya T Fujiwara T Yoneyama T Hayashi H | | Journal: Plant Cell Physiol .
Citation: V : 46 ( 11 ) P : 1779-86 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16120685 Accession (PMID): 16120685 | | Abstract: Sieve tubes are comprised of sieve elements , enucleated cells that are incapable of RNA and protein synthesis .
The proteins in sieve elements are supplied from the neighboring companion cells through plasmodesmata .
In rice plants , it was unclear whether or not all proteins produced in companion cells had the same distribution pattern in the sieve element-companion cell complex In this study , the distribution pattern of four proteins , beta-glucuronidase ( GUS ) , green fluorescent protein ( GFP ) , thioredoxin h ( TRXh ) and glutathione S-transferase ( GST ) were analyzed .
The foreign proteins GUS and GFP were expressed in transgenic rice plants under the control of the TRXh gene promoter ( PTRXh ) , a companion cell-specific promoter .
Analysis of leaf cross-sections of PTRXh-GUS and PTRXh-GFP plants indicated high accumulation of GUS and GFP , respectively , in companion cells rather than in sieve elements .
GUS and GFP were also detected in phloem sap collected from leaf sheaths of the transgenic rice plants , suggesting these proteins could enter sieve elements .
Relative amounts of GFP and endogenous phloem proteins , TRXh and GST , in phloem sap and total leaf extracts were compared .
Compared to TRXh and GST , GFP content was higher in total leaf extracts , but lower in phloem sap , suggesting that GFP accumulated mainly in companion cells rather than in sieve elements .
On the other hand , TRXh and GST appeared to accumulate in sieve elements rather than in companion cells .
These results indicate the evidence for differential distribution of proteins between sieve elements and companion cells in rice plants . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Analysis of leaf cross-sections of PTRXh-GUS and PTRXh-GFP plants indicated high accumulation of GUS and GFP , respectively , in companion cells rather than in sieve elements .
[ Sen. 4, subscore: 1.00 ]: The foreign proteins GUS and GFP were expressed in transgenic rice plants under the control of the TRXh gene promoter ( PTRXh ) , a companion cell-specific promoter .
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Score: 3.00 |
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| Title: Homologs of ToxB , a host-selective toxin gene from Pyrenophora tritici-repentis , are present in the genome of sister-species Pyrenophora bromi and other members of the Ascomycota .
| | Author: Andrie RM Schoch CL Hedges R Spatafora JW Ciuffetti LM | | Journal: Fungal Genet Biol Citation: V : 45 P : 363-77 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18226934 Accession (PMID): 18226934 | | Abstract: Pyrenophora tritici-repentis requires the production of host-selective toxins ( HSTs ) to cause the disease tan spot of wheat , including Ptr ToxA , Ptr ToxB , and Ptr ToxC .
Pyrenophora bromi , the species most closely related to P tritici-repentis , is the causal agent of brown leaf spot of bromegrass .
Because of the relatedness of P bromi and P tritici-repentis , we investigated the possibility that P bromi contains sequences homologous to ToxA and/or ToxB , the products of which may be involved in its interaction with bromegrass .
Multiplex polymerase chain reaction ( PCR ) revealed the presence of ToxB-like sequences in P bromi and high-fidelity PCR was used to clone several of these loci , which were subsequently confirmed to be homologous to ToxB .
Additionally , Southern analysis revealed ToxB from P bromi to have a multicopy nature similar to ToxB from P tritici-repentis .
A combination of phylogenetic and Southern analyses revealed that the distribution of ToxB extends further into the Pleosporaceae , and a search of available fungal genomes identified a distant putative homolog in Magnaporthe grisea , causal agent of rice blast Thus , unlike most described HSTs , ToxB homologs are present across a broad range of plant pathogenic ascomycetes , suggesting that it may have arose in an early ancestor of the Ascomycota .
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[ Sen. 1, subscore: 3.00 ]: Pyrenophora tritici-repentis requires the production of host-selective toxins ( HSTs ) to cause the disease tan spot of wheat , including Ptr ToxA , Ptr ToxB , and Ptr ToxC .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
