| 3 matches found in 3 documents. Search time: 0.021 seconds. |
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| Title: Rice cystatin : bacterial expression , purification , cysteine proteinase inhibitory activity , and insect growth suppressing activity of a truncated form of the protein .
| | Author: Chen MS Johnson B Wen L Muthukrishnan S Kramer KJ Morgan TD Reeck GR .
| | Journal: Protein Expr . Purif . Citation: V : 3 ( 1 ) P : 41-9 Year: 1992 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub1422207 Accession (PMID): 1422207 | | Abstract: A cDNA clone that encodes oryzacystatin , a cysteine protease inhibitor from rice , was isolated and expressed in Escherichia coli BL-21 ( DE3 ) using an expression plasmid under the control of a T7 RNA polymerase promoter .
The construct pT7OC 9b encoded a fusion protein containing 11 amino acid residues of the NH2 terminus of the bacterial protein phi 10 and 79 residues of oryzacystatin lacking 23 NH2-terminal residues of the wild-type protein .
Recombinant oryzacystatin ( ROC ) constituted approximately 10% of the total bacterial protein mass and was purified in a single step by anion-exchange chromatography .
The inhibitory activity of ROC toward papain ( Ki = 3 x 10 ( -8 ) M ) was comparable with that of the naturally occurring protein isolated from rice .
Caseinolytic activity in midgut homogenates from seven species of stored product insects was inhibited from 18 to 85% by ROC , whereas the same activity was inhibited from 14 to 69% by the serine proteinase inhibitor phenylmethylsulfonyl fluoride .
Midguts of stored product insects apparently contain both cysteine proteinases and serine proteinases , but the relative amounts vary with the species .
When fed to the red flour beetle , Tribolium castaneum , 10 wt% ROC in the diet suppressed growth approximately 35% relative to that of the control group of insects . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The construct pT7OC 9b encoded a fusion protein containing 11 amino acid residues of the NH2 terminus of the bacterial protein phi 10 and 79 residues of oryzacystatin lacking 23 NH2-terminal residues of the wild-type protein .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: High level production of the Magnaporthe grisea fructose 1 , 6-bisphosphate aldolase enzyme in Escherichia coli using a small volume bench-top fermentor .
| | Author: LabbEG Bezaire J de Groot S How C Rasmusson T Yaeck J Jervis E Dmitrienko GI Guillemette JG .
| | Journal: Protein Expr . Purif . Citation: V : 51 ( 1 ) P : 110-9 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16901716 Accession (PMID): 16901716 | | Abstract: The Class II fructose 1 , 6-bisphosphate aldolase from the Rice Blast causative agent Magnaporthe grisea was subcloned in the Escherichia coli vector pT7-7 .
The enzyme was overexpressed using fed-batch fermentation in a small bench-top reactor .
A total of 275 g of cells and 1 . 3 g of highly purified enzyme with a specific activity of 70 U/mg were obtained from a 1 . 5L culture .
The purified enzyme is a homodimer of 39 . 6 kDa subunits with a zinc ion at the active site .
Kinetic characterization indicates that the enzyme has a K ( m ) of 51 microM , a k ( cat ) of 46 s ( -1 ) , and a pH optimum of 7 . 8 for fructose 1 , 6-bisphosphate cleavage .
The fermentation system procedure reported exemplifies the potential of using a lab-scale bioreactor for the large scale production of recombinant enzymes .
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[ Sen. 1, subscore: 1.00 ]: The Class II fructose 1 , 6-bisphosphate aldolase from the Rice Blast causative agent Magnaporthe grisea was subcloned in the Escherichia coli vector pT7-7 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: Genetic diversity of N2-fixing bacteria associated with rice roots by molecular evolutionary analysis of a nifD library .
| | Author: Ueda T Suga Y Yahiro N Matsuguchi T | | Journal: Can .
J Microbiol .
Citation: V : 41 ( 3 ) P : 235-40 Year: 1995 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub7736355 Accession (PMID): 7736355 | | Abstract: The rhizosphere of wetland rice has significant N2-fixing activity .
It has been suggested that N2 fixation in the rice root zone is associated with the activity of various N2-fixing heterotrophic bacteria that inhabit the rice rhizosphere .
Because of the generic diversity , many different isolation media and conditions are required to count and isolate these bacteria .
In an attempt to overcome any bias from culture-dependent methods we amplified nifD segments from crude rice root DNA by the polymerase chain reaction .
The nifD fragments were then cloned into a pT7 Blue T-vector to construct a nifD library .
Sixteen cloned nifD genes chosen at random from the library were sequenced .
A comparison with published sequences indicated the presence of seven novel groups of NifD proteins , which implies the existence of at least seven components in the diazotrophic community of rice roots , dominated mainly by proteobacteria .
We also observed genetic variability within the clusters , which suggests the coexistence of many closely related bacterial lineages .
However , we did not find Azospirillum-like nifD clones , although many reports indicated the widespread presence of Azospirillum spp .
Therefore , it remains to be clarified whether Azospirillum species are the widespread N2-fixing bacteria in rice roots . | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: The nifD fragments were then cloned into a pT7 Blue T-vector to construct a nifD library .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
