| 14 matches found in 3 documents. Search time: 0.241 seconds. |
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Score: 8.00 |
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| Title: Pollen semi-sterility1 encodes a kinesin-1-like protein important for male meiosis , anther dehiscence , and fertility in rice .
| | Author: Zhou S Wang Y Li W Zhao Z Ren Y Wang Y Gu S Lin Q Wang D Jiang L Su N Zhang X Liu L Cheng Z Lei C Wang J Guo X Wu F Ikehashi H Wang H Wan J | | Journal: Plant Cell Citation: V : 23 P : 111-29 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21282525 Accession (PMID): 21282525 | | Abstract: In flowering plants , male meiosis produces four microspores , which develop into pollen grains and are released by anther dehiscence to pollinate female gametophytes .
The molecular and cellular mechanisms regulating male meiosis in rice ( Oryza sativa ) remain poorly understood .
Here , we describe a rice pollen semi-sterility1 ( pss1 ) mutant , which displays reduced spikelet fertility ( 40% ) primarily caused by reduced pollen viability ( 50% viable ) , and defective anther dehiscence .
Map-based molecular cloning revealed that PSS1 encodes a kinesin-1-like protein .
PSS1 is broadly expressed in various organs , with highest expression in panicles .
Furthermore , PSS1 expression is significantly upregulated during anther development and peaks during male meiosis .
The PSS1-green fluorescent protein fusion is predominantly localized in the cytoplasm of rice protoplasts .
Substitution of a conserved Arg ( Arg-289 ) to His in the PSS1 motor domain nearly abolishes its microtubule-stimulated ATPase activity .
Consistent with this , lagging chromosomes and chromosomal bridges were found at anaphase I and anaphase II of male meiosis in the pss1 mutant .
Together , our results suggest that PSS1 defines a novel member of the kinesin-1 family essential for male meiotic chromosomal dynamics , male gametogenesis , and anther dehiscence in rice .
| Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Here , we describe a rice pollen semi-sterility1 ( pss1 ) mutant , which displays reduced spikelet fertility ( 40% ) primarily caused by reduced pollen viability ( 50% viable ) , and defective anther dehiscence .
[ Sen. 4, subscore: 1.00 ]: Map-based molecular cloning revealed that PSS1 encodes a kinesin-1-like protein .
[ Sen. 5, subscore: 1.00 ]: PSS1 is broadly expressed in various organs , with highest expression in panicles .
[ Sen. 6, subscore: 1.00 ]: Furthermore , PSS1 expression is significantly upregulated during anther development and peaks during male meiosis .
[ Sen. 7, subscore: 1.00 ]: The PSS1-green fluorescent protein fusion is predominantly localized in the cytoplasm of rice protoplasts .
[ Sen. 8, subscore: 1.00 ]: Substitution of a conserved Arg ( Arg-289 ) to His in the PSS1 motor domain nearly abolishes its microtubule-stimulated ATPase activity .
[ Sen. 9, subscore: 1.00 ]: Consistent with this , lagging chromosomes and chromosomal bridges were found at anaphase I and anaphase II of male meiosis in the pss1 mutant .
[ Sen. 10, subscore: 1.00 ]: Together , our results suggest that PSS1 defines a novel member of the kinesin-1 family essential for male meiotic chromosomal dynamics , male gametogenesis , and anther dehiscence in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: Fine mapping of pss1 , a pollen semi-sterile gene in rice ( Oryza sativa L ) .
| | Author: Li W Jiang L Zhou S Wang C Liu L Chen L Ikehashi H Wan J | | Journal: Theor . Appl . Genet . Citation: V : 114 ( 5 ) P : 939-46 Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17279367 Accession (PMID): 17279367 | | Abstract: During routine seed increase procedures in rice , semi-sterile plants are common ; however , such semi-sterility mutants in rice varieties have been only rarely analyzed genetically .
W207-2 is a semi-sterile selection from the japonica rice variety Nipponbare .
In this report , we found the female gamete of W207-2 was normal , and its semi-sterility was unaffected by growth duration but was conditioned by a recessive nuclear gene whose action leads to pollen semi-sterility and anther indehiscence , and the gene was named as pss1 ( pollen semi-sterile ) .
Using an F ( 2 ) population derived from the two parents W207-2 and Dular and a pooled DNA strategy , pss1 was mapped to an interval on chromosome 8 defined by the two SSR loci RM6356 and RS41 .
The position of pss1 was confirmed in another F ( 2 ) population derived from the cross W207-2 x Nipponbare .
Over 2 , 000 homozygous pss1 segregants from the large W207-2 x Dular F ( 2 ) population were used to fine map pss1 to a 0 . 04 cM segment flanked by a CAPs marker L2 and a dCAPs L3 marker .
Sequences for both markers are present on a single PAC clone , and the physical distance between them is about 28 kb .
Analysis of the PAC sequence predicts the presence of five open reading frames , they are as follows : putative ribonuclease PH , putative avr9 elicitor response protein , kinesin1-like protein , putative protein RNP-D precursor and putative 40S ribosomal protein S13 .
This result would be helpful in cloning the pss1 gene .
| Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Over 2 , 000 homozygous pss1 segregants from the large W207-2 x Dular F ( 2 ) population were used to fine map pss1 to a 0 . 04 cM segment flanked by a CAPs marker L2 and a dCAPs L3 marker .
[ Sen. 3, subscore: 1.00 ]: In this report , we found the female gamete of W207-2 was normal , and its semi-sterility was unaffected by growth duration but was conditioned by a recessive nuclear gene whose action leads to pollen semi-sterility and anther indehiscence , and the gene was named as pss1 ( pollen semi-sterile ) .
[ Sen. 4, subscore: 1.00 ]: Using an F ( 2 ) population derived from the two parents W207-2 and Dular and a pooled DNA strategy , pss1 was mapped to an interval on chromosome 8 defined by the two SSR loci RM6356 and RS41 .
[ Sen. 5, subscore: 1.00 ]: The position of pss1 was confirmed in another F ( 2 ) population derived from the cross W207-2 x Nipponbare .
[ Sen. 9, subscore: 1.00 ]: This result would be helpful in cloning the pss1 gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Fine mapping of pss1 , a pollen semi-sterile gene in rice ( Oryza sativa L ) .
| | Author: Li W Jiang L Zhou S Wang C Liu L Chen L Ikehashi H Wan J | | Journal: Theor . Appl . Genet . Citation: V : 114 ( 5 ) P : 939-46 Year: 2007 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub17279367 Accession (PMID): 17279367 | | Abstract: During routine seed increase procedures in rice , semi-sterile plants are common ; however , such semi-sterility mutants in rice varieties have been only rarely analyzed genetically .
W207-2 is a semi-sterile selection from the japonica rice variety Nipponbare .
In this report , we found the female gamete of W207-2 was normal , and its semi-sterility was unaffected by growth duration but was conditioned by a recessive nuclear gene whose action leads to pollen semi-sterility and anther indehiscence , and the gene was named as pss1 ( pollen semi-sterile ) .
Using an F ( 2 ) population derived from the two parents W207-2 and Dular and a pooled DNA strategy , pss1 was mapped to an interval on chromosome 8 defined by the two SSR loci RM6356 and RS41 .
The position of pss1 was confirmed in another F ( 2 ) population derived from the cross W207-2 x Nipponbare .
Over 2 , 000 homozygous pss1 segregants from the large W207-2 x Dular F ( 2 ) population were used to fine map pss1 to a 0 . 04 cM segment flanked by a CAPs marker L2 and a dCAPs L3 marker .
Sequences for both markers are present on a single PAC clone , and the physical distance between them is about 28 kb .
Analysis of the PAC sequence predicts the presence of five open reading frames , they are as follows : putative ribonuclease PH , putative avr9 elicitor response protein , kinesin1-like protein , putative protein RNP-D precursor and putative 40S ribosomal protein S13 .
This result would be helpful in cloning the pss1 gene .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Fine mapping of pss1 , a pollen semi-sterile gene in rice ( Oryza sativa L ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
