Score: 7.00 |
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| Title: Delimitation of the PSH1 ( t ) gene for rice purple leaf sheath to a 23 . 5 kb DNA fragment .
| | Author: Wang WY Ding HF Li GX Jiang MS Li RF Liu X Zhang Y Yao FY | | Journal: Genome Citation: V : 52 P : 268-74 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19234555 Accession (PMID): 19234555 | | Abstract: Leaf sheath color plays an important role as a marker for rice genetic improvement .
A recombinant inbred line ( RIL ) population consisting of 220 individuals was developed from a cross between an Oryza sativa subsp . indica variety , IRBB60 , and an Oryza sativa subsp . japonica variety , 9407 .
Within the RIL population , a line , RI51 , was found to have purple leaf sheath ( PSH ) .
To map the gene governing PSH , RI51 was crossed with 9407 green leaf sheath ( GSH ) to develop an F2 segregating population .
The distribution of F2 plants with PSH and GSH fitted a segregation ratio of 3 : 1 , indicating that the PSH was controlled by a major dominant gene .
The gene locus for PSH , tentatively designated as PSH1 ( t ) , was identified by surveying two bulks made of the respective 40 individuals with PSH and GSH with SSR markers covering the entire rice genome .
The survey indicated that the PSH1 ( t ) region was located on chromosome 1 .
Further confirmation was made using a large random sample of 360 individuals from the same F2 population and the PSH1 ( t ) locus was then mapped on chromosome 1 between SSR markers RM3475 and RM7202 with genetic distances of 2 . 0 and 1 . 1 cM , respectively .
For fine mapping of PSH1 ( t ) , a large F ( 2 : 3 ) segregating population with 3300 individuals from the seven heterozygous F2 plants in the RM3475-RM7202 region was constructed .
Analysis of recombinants in the PSH1 ( t ) region anchored the gene locus to an interval of 23 . 5 kb flanked by the left marker L03 and the right marker L05 .
Sequence analysis of this fragment predicted six open reading frames encoding a putative trans-sialidase , a putative Plastidic ATP/ADP-transporter , and four unknown proteins .
The detailed genetic and physical maps of the PSH1 ( t ) locus will be very useful in molecular cloning of the PSH1 ( t ) gene .
| Matching Sentences:
[ Sen. 12, subscore: 2.00 ]: The detailed genetic and physical maps of the PSH1 ( t ) locus will be very useful in molecular cloning of the PSH1 ( t ) gene .
[ Sen. 6, subscore: 1.00 ]: The gene locus for PSH , tentatively designated as PSH1 ( t ) , was identified by surveying two bulks made of the respective 40 individuals with PSH and GSH with SSR markers covering the entire rice genome .
[ Sen. 7, subscore: 1.00 ]: The survey indicated that the PSH1 ( t ) region was located on chromosome 1 .
[ Sen. 8, subscore: 1.00 ]: Further confirmation was made using a large random sample of 360 individuals from the same F2 population and the PSH1 ( t ) locus was then mapped on chromosome 1 between SSR markers RM3475 and RM7202 with genetic distances of 2 . 0 and 1 . 1 cM , respectively .
[ Sen. 9, subscore: 1.00 ]: For fine mapping of PSH1 ( t ) , a large F ( 2 : 3 ) segregating population with 3300 individuals from the seven heterozygous F2 plants in the RM3475-RM7202 region was constructed .
[ Sen. 10, subscore: 1.00 ]: Analysis of recombinants in the PSH1 ( t ) region anchored the gene locus to an interval of 23 . 5 kb flanked by the left marker L03 and the right marker L05 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Delimitation of the PSH1 ( t ) gene for rice purple leaf sheath to a 23 . 5 kb DNA fragment .
| | Author: Wang WY Ding HF Li GX Jiang MS Li RF Liu X Zhang Y Yao FY | | Journal: Genome Citation: V : 52 P : 268-74 Year: 2009 Type: In-Process | | Literature: oryza Field: title Doc ID: pub19234555 Accession (PMID): 19234555 | | Abstract: Leaf sheath color plays an important role as a marker for rice genetic improvement .
A recombinant inbred line ( RIL ) population consisting of 220 individuals was developed from a cross between an Oryza sativa subsp . indica variety , IRBB60 , and an Oryza sativa subsp . japonica variety , 9407 .
Within the RIL population , a line , RI51 , was found to have purple leaf sheath ( PSH ) .
To map the gene governing PSH , RI51 was crossed with 9407 green leaf sheath ( GSH ) to develop an F2 segregating population .
The distribution of F2 plants with PSH and GSH fitted a segregation ratio of 3 : 1 , indicating that the PSH was controlled by a major dominant gene .
The gene locus for PSH , tentatively designated as PSH1 ( t ) , was identified by surveying two bulks made of the respective 40 individuals with PSH and GSH with SSR markers covering the entire rice genome .
The survey indicated that the PSH1 ( t ) region was located on chromosome 1 .
Further confirmation was made using a large random sample of 360 individuals from the same F2 population and the PSH1 ( t ) locus was then mapped on chromosome 1 between SSR markers RM3475 and RM7202 with genetic distances of 2 . 0 and 1 . 1 cM , respectively .
For fine mapping of PSH1 ( t ) , a large F ( 2 : 3 ) segregating population with 3300 individuals from the seven heterozygous F2 plants in the RM3475-RM7202 region was constructed .
Analysis of recombinants in the PSH1 ( t ) region anchored the gene locus to an interval of 23 . 5 kb flanked by the left marker L03 and the right marker L05 .
Sequence analysis of this fragment predicted six open reading frames encoding a putative trans-sialidase , a putative Plastidic ATP/ADP-transporter , and four unknown proteins .
The detailed genetic and physical maps of the PSH1 ( t ) locus will be very useful in molecular cloning of the PSH1 ( t ) gene .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Delimitation of the PSH1 ( t ) gene for rice purple leaf sheath to a 23 . 5 kb DNA fragment .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
