| 11 matches found in 8 documents. Search time: 0.404 seconds. |
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| Title: Pathogen resistance of transgenic rice plants expressing mitogen-activated protein kinase 1 , MK1 , from Capsicum annuum .
| | Author: Lee DE Lee IJ Han O Baik MG Han SS Back K | | Journal: Mol .
Cells Citation: V : 17 ( 1 ) P : 81-5 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15055532 Accession (PMID): 15055532 | | Abstract: Mitogen-activated protein kinase 1 ( MK1 ) from Capsicum annuum was previously cloned and characterized .
MK1 is highly conserved in plants and its amino acid sequence is 92% identical to wound-inducible protein kinase ( WIPK ) from tobacco In C annuum , MK1 is transcriptionally activated by wounding .
In the present work , the MK1 gene was introduced into the rice genome by Agrobacterium-mediated transformation .
Seven independent transgenic rice plants were isolated and characterized .
All seven lines had a single-copy insertion of the MK1 transgene .
MK1 mRNA and protein were detected in the transgenic rice , but not in wild type rice .
In unwounded transgenic rice plants , the level of jasmonic acid was 3-fold higher than in the wild type , whereas in wounded leaves the level of jasmonic acid was the same in transgenic and wild type plants .
Expression of the wound-inducible pathogenesis-related gene PR1a was also higher in transgenic than in wild type plants .
Either overexpression of PR1a or increased PR1b and PR10 expression in transgenic rice plants may confer increased resistance to rice blast | Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: Expression of the wound-inducible pathogenesis-related gene PR1a was also higher in transgenic than in wild type plants .
[ Sen. 9, subscore: 1.00 ]: Either overexpression of PR1a or increased PR1b and PR10 expression in transgenic rice plants may confer increased resistance to rice blast
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| Title: Expression of defense genes and antioxidant defense responses in rice resistance to neck blast at the preliminary heading stage and full heading stage .
| | Author: Hao Z Wang L Huang F Tao R | | Journal: Plant Physiol Biochem Citation: V : 57 P : 222-30 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22738867 Accession (PMID): 22738867 | | Abstract: The rice variety Xiushui227 is resistant to neck blast at three crucial panicle stages ( the booting stage , the preliminary heading stage ( PHS ) , and the full heading stage ( FHS ) ) that controlling neck blast The other rice variety Liangyou6326 is susceptible to neck blast at these three panicle stages .
These two varieties were used to analyze the expression patterns of defense genes and antioxidant defense responses at the PHS and FHS , after inoculating the necks in vitro with Magnaporthe oryzae , respectively .
All defense genes ( pathogenesis-related genes PR1a , Gns1 ( 1 , 3 ; 1 , 4-beta-glucanase ) , Cht-1 ( chitinase ) , PR4 , PR5 , and PR10a , secondary metabolite pathway genes PAL ( phenylalanine ammonia-lyase ) , CHS ( chalcone synthase ) , and LOX ( lipoxygenase ) , and oxidative stress-related protein genes POX22 . 3 ( peroxidase ) , and PPO ( polyphenol oxidase ) ) used in this experiment except Cht-1 , CHS and PPO could mainly play important roles in the resistance to neck blast at the PHS in Xiushui227 , and CHS and PPO may primarily participate in fighting back against to neck blast at the FHS .
Conversely , PR1a , Cht-1 , PR4 , PR10a , CHS , LOX-RLL , and PPO could chiefly play participate in defending Liangyou6326 against neck blast at the FHS , and PR5 , PAL , and POX22 . 3 may be primarily involved in fighting back against to neck blast at the PHS .
Furthermore , the antioxidant enzymes superoxide dismutase , peroxidase , and catalase may mainly participate in defending Xiushui227 against neck blast at the PHS and defending Liangyou6326 against neck blast at the FHS , respectively .
Therefore oxidative damage is less at the PHS in Xiushui227 and at the FHS in Liangyou6326 , respectively .
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[ Sen. 3, subscore: 1.00 ]: All defense genes ( pathogenesis-related genes PR1a , Gns1 ( 1 , 3 ; 1 , 4-beta-glucanase ) , Cht-1 ( chitinase ) , PR4 , PR5 , and PR10a , secondary metabolite pathway genes PAL ( phenylalanine ammonia-lyase ) , CHS ( chalcone synthase ) , and LOX ( lipoxygenase ) , and oxidative stress-related protein genes POX22 . 3 ( peroxidase ) , and PPO ( polyphenol oxidase ) ) used in this experiment except Cht-1 , CHS and PPO could mainly play important roles in the resistance to neck blast at the PHS in Xiushui227 , and CHS and PPO may primarily participate in fighting back against to neck blast at the FHS .
[ Sen. 4, subscore: 1.00 ]: Conversely , PR1a , Cht-1 , PR4 , PR10a , CHS , LOX-RLL , and PPO could chiefly play participate in defending Liangyou6326 against neck blast at the FHS , and PR5 , PAL , and POX22 . 3 may be primarily involved in fighting back against to neck blast at the PHS .
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| Title: Abnormal cell divisions in leaf primordia caused by the expression of the rice homeobox gene OSH1 lead to altered morphology of leaves in transgenic tobacco | | Author: Sato Y Tamaoki M Murakami T Yamamoto N Kano-Murakami Y Matsuoka M | | Journal: Mol . Gen . Genet . Citation: V : 251 ( 1 ) P : 13-22 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8628242 Accession (PMID): 8628242 | | Abstract: Transgenic tobacco plants were generated carrying a rice homeobox gene , OSH1 , controlled by the promoter of a gene encoding a tobacco pathogenesis-related protein ( PR1a ) .
These lines were morphologically abnormal , with wrinkled and/or lobed leaves .
Histological analysis of shoot apex primordia indicates arrest of lateral leaf blade expansion , often resulting in asymmetric and anisotrophic growth of leaf blades .
Other notable abnormalities included abnormal or arrested development of leaf lateral veins .
Interestingly , OHS1 expression was undetectable in mature leaves with the aberrant morphological features .
Thus , OSH1 expression in mature leaves is not necessary for abnormal leaf development .
Northern blot and in situ hybridization analyses indicate that PR1a-OSH1 is expressed only in the shoot apical meristem and in very young leaf primordia .
Therefore , the aberrant morphological features are an indirect consequence of ectopic OSH1 gene expression .
The only abnormality observed in it issues expressing the transgene was periclinal ( rather than anticlinal ) division in mesophyll cells during leaf blade initiation .
This generates thicker leaf blades and disrupts the mesophyll cell layers , from which vascular it issues differentiate .
The OSH1 product appears to affect the mechanism controlling the orientation of the plane of cell division , resulting in abnormal periclinal division of mesophyll cell , which in turn results in the gross morphological abnormalities observed in the transgenic lines . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Transgenic tobacco plants were generated carrying a rice homeobox gene , OSH1 , controlled by the promoter of a gene encoding a tobacco pathogenesis-related protein ( PR1a ) .
[ Sen. 7, subscore: 1.00 ]: Northern blot and in situ hybridization analyses indicate that PR1a-OSH1 is expressed only in the shoot apical meristem and in very young leaf primordia .
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| Title: Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level , PR gene expression , and host resistance to fungal infection .
| | Author: Mei C Qi M Sheng G Yang Y | | Journal: Mol .
Plant Microbe Interact .
Citation: V : 19 ( 10 ) P : 1127-37 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17022177 Accession (PMID): 17022177 | | Abstract: Many studies in dicotyledonous plants have shown that jasmonates , including jasmonic acid ( JA ) and methyl jasmonate , are important signal molecules involved in induced resistance to pathogen infection and insect herbivory .
However , very little genetic and molecular evidence is available to demonstrate their role in host defense response of rice and other economically important monocot plants .
In this study , we have shown that exogenous application of JA was able to activate defense gene expression and local induced resistance in rice seedlings against the rice blast fungus ( Magnaporthe grisea ) .
Furthermore , we have characterized a pathogen-inducible rice OsAOS2 gene ( which encodes allene oxide synthase , a key enzyme in the JA biosynthetic pathway ) and examined the role of endogenous JA in rice defense response through transgenic manipulation of the JA biosynthesis .
Sequence analysis indicated that OsAOS2 contains four common domains of the cytochrome P450 enzyme , but does not have the signal peptide for chloroplast targeting .
The basal level of OsAOS2 expression is very low in leaves but relatively high in the sheath , culm , and flower of rice plants .
Interestingly , the expression of OsAOS2 in rice leaves can be induced significantly upon M grisea infection .
Transgenic rice lines carrying the OsAOS2 transgene under the control of a strong , pathogen-inducible PBZ1 promoter accumulated abundant OsAOS2 transcripts and higher levels of JA , especially after the pathogen infection .
These transgenic lines also exhibited enhanced activation of pathogenesis-related ( PR ) genes such as PR1a , PR3 , and PR5 and increased resistance to M grisea infection .
Our results suggest that JA plays a significant role in PR gene induction and blast resistance in rice plants . | Matching Sentences:
[ Sen. 9, subscore: 1.00 ]: These transgenic lines also exhibited enhanced activation of pathogenesis-related ( PR ) genes such as PR1a , PR3 , and PR5 and increased resistance to M grisea infection .
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| Title: Opposite functions of a rice mitogen-activated protein kinase during the process of resistance against Xanthomonas oryzae .
| | Author: Shen X Yuan B Liu H Li X Xu C Wang S | | Journal: Plant J Citation: V : 64 P : 86-99 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20663089 Accession (PMID): 20663089 | | Abstract: The pathogen-induced plant defense signaling network consists of multiple components , although only some of them are characterized .
Most of the known components function either as activators or repressors in host-pathogen interactions .
Here we report that a mitogen-activated protein kinase , OsMPK6 , functions both as an activator and a repressor in rice resistance against Xanthomonas oryzae pv . oryzae ( Xoo ) , the causal organism of bacterial blight disease .
Activation of OsMPK6 resulted in the formation of lesion mimics and local resistance to Xoo , accompanied by the accumulation of salicylic acid ( SA ) and jasmonic acid ( JA ) , and the induced expression of SA and JA-signaling genes .
Nuclear localization of OsMPK6 was essential for local resistance , suggesting that modulating the expression of defense-responsive genes through transcription regulators may be the primary mechanism of OsMPK6-mediated local resistance .
The knock-out of OsMPK6 resulted in enhanced Xoo resistance , increased accumulation of SA and enhanced resistance to X oryzae pv . oryzicola , the causal organism of bacterial streak disease , in systemic it issues .
Xoo infection induced the expression of PR1a , the marker gene of systemic acquired resistance ( SAR ) , in systemic health it issues of OsMPK6-knock-out plants .
These results suggest that OsMPK6 negatively regulates SAR .
Thus OsMPK6 is a two-faced player in the rice-Xoo interaction .
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[ Sen. 7, subscore: 1.00 ]: Xoo infection induced the expression of PR1a , the marker gene of systemic acquired resistance ( SAR ) , in systemic health it issues of OsMPK6-knock-out plants .
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