| 37 matches found in 10 documents. Search time: 0.802 seconds. |
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Score: 12.00 |
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| Title: Plant low-molecular-weight phospholipase A2S ( PLA2s ) are structurally related to the animal secretory PLA2s and are present as a family of isoforms in rice ( Oryza sativa ) .
| | Author: Sthl U Lee M Sjdahl S Archer D Cellini F Ek B Iannacone R MacKenzie D Semeraro L Tramontano E Stymme S | | Journal: Plant Mol . Biol . Citation: V : 41 ( 4 ) P : 481-90 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10608658 Accession (PMID): 10608658 | | Abstract: Recently , we purified to homogeneity and characterized a low-molecular-weight calcium-dependent phospholipase A2 ( PLA2 ) from developing elm seed endosperm .
This represented the first purified and characterized PLA2 from a plant it issue .
The full sequences of two distinct but homologous rice ( Oryza sativa ) cDNAs are given here .
These encode mature proteins of 1 19 amino acids ( PLA2-I , preceded by a 19 amino acid signal peptide ) and 128 amino acids ( PLA2-II . preceded by a 25 amino acid signal peptide ) , and were derived from four expressed sequence tag ( EST ) clones .
Both proteins were homologous to the N-terminal amino acid sequence of the elm PLA2 .
They contained twelve conserved cysteine residues and sequences that are likely to represent the Ca ( 2+ ) -binding loop and active-site motif , which are characteristic of animal secretory PLA2s .
A soluble PLA2s activity was purified 145 000-fold from green rice shoots .
This had the same biochemical characteristics as the elm and animal secretory PLA2s .
The purified rice PLA2 consisted of two proteins , with a molecular weight of 12 440 and 12 920 , that had identical N-terminal amino acid sequences .
This sequence was different from but homologous to the PLA2-I and PLA2-II sequences .
Taken together , the results suggest that at least three different low-molecular-weight PLA2s are expressed in green rice shoots .
Southern blot analysis suggested that multiple copies of such genes are likely to occur in the rice and in other plant genomes . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: These encode mature proteins of 1 19 amino acids ( PLA2-I , preceded by a 19 amino acid signal peptide ) and 128 amino acids ( PLA2-II . preceded by a 25 amino acid signal peptide ) , and were derived from four expressed sequence tag ( EST ) clones .
[ Sen. 10, subscore: 2.00 ]: This sequence was different from but homologous to the PLA2-I and PLA2-II sequences .
[ Sen. 1, subscore: 1.00 ]: Recently , we purified to homogeneity and characterized a low-molecular-weight calcium-dependent phospholipase A2 ( PLA2 ) from developing elm seed endosperm .
[ Sen. 2, subscore: 1.00 ]: This represented the first purified and characterized PLA2 from a plant it issue .
[ Sen. 5, subscore: 1.00 ]: Both proteins were homologous to the N-terminal amino acid sequence of the elm PLA2 .
[ Sen. 6, subscore: 1.00 ]: They contained twelve conserved cysteine residues and sequences that are likely to represent the Ca ( 2+ ) -binding loop and active-site motif , which are characteristic of animal secretory PLA2s .
[ Sen. 7, subscore: 1.00 ]: A soluble PLA2s activity was purified 145 000-fold from green rice shoots .
[ Sen. 8, subscore: 1.00 ]: This had the same biochemical characteristics as the elm and animal secretory PLA2s .
[ Sen. 9, subscore: 1.00 ]: The purified rice PLA2 consisted of two proteins , with a molecular weight of 12 440 and 12 920 , that had identical N-terminal amino acid sequences .
[ Sen. 11, subscore: 1.00 ]: Taken together , the results suggest that at least three different low-molecular-weight PLA2s are expressed in green rice shoots .
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Score: 11.00 |
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| Title: Rice PLASTOCHRON genes regulate leaf maturation downstream of the gibberellin signal transduction pathway .
| | Author: Mimura M Nagato Y Itoh J | | Journal: Planta Citation: V : 235 P : 1081-9 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22476293 Accession (PMID): 22476293 | | Abstract: Rice PLASTOCHRON 1 ( PLA1 ) and PLA2 genes regulate leaf maturation and plastochron , and their loss-of-function mutants exhibit small organs and rapid leaf emergence .
They encode a cytochrome P450 protein CYP78A11 and an RNA-binding protein , respectively .
Their homologs in Arabidopsis and maize are also associated with plant development/organ size .
Despite the importance of PLA genes in plant development , their molecular functions remain unknown .
Here , we investigated how PLA1 and PLA2 genes are related to phytohormones .
We found that gibberellin ( GA ) is the major phytohormone that promotes PLA1 and PLA2 expression .
GA induced PLA1 and PLA2 expression , and conversely the GA-inhibitor uniconazole suppressed PLA1 and PLA2 expression .
In pla1-4 and pla2-1 seedlings , expression levels of GA biosynthesis genes and the signal transduction gene were similar to those in wild-type seedlings .
GA treatment slightly down-regulated the GA biosynthesis gene GA20ox2 and up-regulated the GA-catabolizing gene GA2ox4 , whereas the GA biosynthesis inhibitor uniconazole up-regulated GA20ox2 and down-regulated GA2ox4 both in wild-type and pla mutants , suggesting that the GA feedback mechanism is not impaired in pla1 and pla2 .
To reveal how GA signal transduction affects the expression of PLA1 and PLA2 , PLA expression in GA-signaling mutants was examined .
In GA-insensitive mutant , gid1 and less-sensitive mutant , Slr1-d1 , PLA1 and PLA2 expression was down-regulated .
On the other hand , the expression levels of PLA1 and PLA2 were highly enhanced in a GA-constitutive-active mutant , slr1-1 , causing ectopic overexpression .
These results indicate that both PLA1 and PLA2 act downstream of the GA signal transduction pathway to regulate leaf development .
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[ Sen. 7, subscore: 2.00 ]: GA induced PLA1 and PLA2 expression , and conversely the GA-inhibitor uniconazole suppressed PLA1 and PLA2 expression .
[ Sen. 1, subscore: 1.00 ]: Rice PLASTOCHRON 1 ( PLA1 ) and PLA2 genes regulate leaf maturation and plastochron , and their loss-of-function mutants exhibit small organs and rapid leaf emergence .
[ Sen. 5, subscore: 1.00 ]: Here , we investigated how PLA1 and PLA2 genes are related to phytohormones .
[ Sen. 6, subscore: 1.00 ]: We found that gibberellin ( GA ) is the major phytohormone that promotes PLA1 and PLA2 expression .
[ Sen. 8, subscore: 1.00 ]: In pla1-4 and pla2-1 seedlings , expression levels of GA biosynthesis genes and the signal transduction gene were similar to those in wild-type seedlings .
[ Sen. 9, subscore: 1.00 ]: GA treatment slightly down-regulated the GA biosynthesis gene GA20ox2 and up-regulated the GA-catabolizing gene GA2ox4 , whereas the GA biosynthesis inhibitor uniconazole up-regulated GA20ox2 and down-regulated GA2ox4 both in wild-type and pla mutants , suggesting that the GA feedback mechanism is not impaired in pla1 and pla2 .
[ Sen. 10, subscore: 1.00 ]: To reveal how GA signal transduction affects the expression of PLA1 and PLA2 , PLA expression in GA-signaling mutants was examined .
[ Sen. 11, subscore: 1.00 ]: In GA-insensitive mutant , gid1 and less-sensitive mutant , Slr1-d1 , PLA1 and PLA2 expression was down-regulated .
[ Sen. 12, subscore: 1.00 ]: On the other hand , the expression levels of PLA1 and PLA2 were highly enhanced in a GA-constitutive-active mutant , slr1-1 , causing ectopic overexpression .
[ Sen. 13, subscore: 1.00 ]: These results indicate that both PLA1 and PLA2 act downstream of the GA signal transduction pathway to regulate leaf development .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
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| Title: PLASTOCHRON2 regulates leaf initiation and maturation in rice .
| | Author: Kawakatsu T Itoh J Miyoshi K Kurata N Alvarez N Veit B Nagato Y | | Journal: Plant Cell Citation: V : 18 ( 3 ) P : 612-25 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16461585 Accession (PMID): 16461585 | | Abstract: In higher plants , leaves initiate in constant spatial and temporal patterns .
Although the pattern of leaf initiation is a key element of plant shoot architecture , little is known about how the time interval between initiation events , termed plastochron , is regulated .
Here , we present a detailed analysis of plastochron2 ( pla2 ) , a rice ( Oryza sativa ) mutant that exhibits shortened plastochron and precocious maturation of leaves during the vegetative phase and ectopic shoot formation during the reproductive phase .
The corresponding PLA2 gene is revealed to be an orthologue of terminal ear1 , a maize ( Zea mays ) gene that encodes a MEI2-like RNA binding protein .
PLA2 is expressed predominantly in young leaf primordia .
We show that PLA2 normally acts to retard the rate of leaf maturation but does so independently of PLA1 , which encodes a member of the P450 family .
Based on these analyses , we propose a model in which plastochron is determined by signals from immature leaves that act non-cell-autonomously in the shoot apical meristem to inhibit the initiation of new leaves . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Here , we present a detailed analysis of plastochron2 ( pla2 ) , a rice ( Oryza sativa ) mutant that exhibits shortened plastochron and precocious maturation of leaves during the vegetative phase and ectopic shoot formation during the reproductive phase .
[ Sen. 4, subscore: 1.00 ]: The corresponding PLA2 gene is revealed to be an orthologue of terminal ear1 , a maize ( Zea mays ) gene that encodes a MEI2-like RNA binding protein .
[ Sen. 5, subscore: 1.00 ]: PLA2 is expressed predominantly in young leaf primordia .
[ Sen. 6, subscore: 1.00 ]: We show that PLA2 normally acts to retard the rate of leaf maturation but does so independently of PLA1 , which encodes a member of the P450 family .
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Score: 3.00 |
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| Title: PLASTOCHRON3/GOLIATH encodes a glutamate carboxypeptidase required for proper development in rice .
| | Author: Kawakatsu T Taramino G Itoh JI Allen J Sato Y Hong SK Yule R Nagasawa N Kojima M Kusaba M Sakakibara H Sakai H Nagato Y | | Journal: Plant J Citation: V : P : Year: 2009 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub19228340 Accession (PMID): 19228340 | | Abstract: Most aerial parts of the plant body are products of the continuous activity of the shoot apical meristem ( SAM ) .
Leaves are the major component of the aerial plant body , and their temporal and spatial distribution mainly determines shoot architecture .
Here we report the identification of the rice gene PLASTOCHRON3 ( PLA3 ) /GOLIATH ( GO ) that regulates various developmental processes including the rate of leaf initiation ( the plastochron ) .
PLA3/GO encodes a glutamate carboxypeptidase , which is thought to catabolize small acidic peptides and produce small signaling molecules . pla3 exhibits similar phenotypes to pla1 and pla2 a shortened plastochron , precocious leaf maturation and rachis branch-to-shoot conversion in the reproductive phase .
However , in contrast to pla1 and pla2 , pla3 showed pleiotropic phenotypes including enlarged embryo , seed vivipary , defects in SAM maintenance and aberrant leaf morphology .
Consistent with these pleiotropic phenotypes , PLA3 is expressed in the whole plant body , and is involved in plant hormone homeostasis .
Double mutant analysis revealed that PLA1 , PLA2 and PLA3 are regulated independently but function redundantly .
Our results suggest that PLA3 modulates various signaling pathways associated with a number of developmental processes .
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[ Sen. 4, subscore: 1.00 ]: PLA3/GO encodes a glutamate carboxypeptidase , which is thought to catabolize small acidic peptides and produce small signaling molecules . pla3 exhibits similar phenotypes to pla1 and pla2 a shortened plastochron , precocious leaf maturation and rachis branch-to-shoot conversion in the reproductive phase .
[ Sen. 5, subscore: 1.00 ]: However , in contrast to pla1 and pla2 , pla3 showed pleiotropic phenotypes including enlarged embryo , seed vivipary , defects in SAM maintenance and aberrant leaf morphology .
[ Sen. 7, subscore: 1.00 ]: Double mutant analysis revealed that PLA1 , PLA2 and PLA3 are regulated independently but function redundantly .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Purification and characterization of a low-molecular-weight phospholipase A2 from developing seeds of elm .
| | Author: Sthl U Ek B Stymne S | | Journal: Plant Physiol .
Citation: V : 117 ( 1 ) P : 197-205 Year: 1998 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9576789 Accession (PMID): 9576789 | | Abstract: Phospholipase A2 ( PLA2 ) was purified about 180 , 000 times compared with the starting soluble-protein extract from developing elm ( Ulmus glabra ) seeds .
On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the purified fraction showed a single protein band with a mobility that corresponded to 15 kD , from which activity could be recovered .
When analyzed by matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry , the enzyme had a deduced mass of 13 , 900 D A 53-amino acid-long N-terminal sequence was determined and aligned with other sequences , giving 62% identity to the deduced amino acid sequence of some rice ( Oryza sativa ) expressed sequence tag clones .
The purified enzyme had an alkaline pH optimum and required Ca2+ for activity .
It was unusually stable with regard to heat , acidity , and organic solvents but was sensitive to disulfide bond-reducing agents .
The enzyme is a true PLA2 , neither hydrolyzing the sn-1 position of phosphatidylcholine nor having any activity toward lysophosphatidylcholine or diacylglycerol .
The biochemical data and amino acid sequence alignments indicate that the enzyme is related to the well-characterized family of animal secretory PLA2s and , to our knowledge , is the first plant enzyme of this type to be described . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Phospholipase A2 ( PLA2 ) was purified about 180 , 000 times compared with the starting soluble-protein extract from developing elm ( Ulmus glabra ) seeds .
[ Sen. 6, subscore: 1.00 ]: The enzyme is a true PLA2 , neither hydrolyzing the sn-1 position of phosphatidylcholine nor having any activity toward lysophosphatidylcholine or diacylglycerol .
[ Sen. 7, subscore: 1.00 ]: The biochemical data and amino acid sequence alignments indicate that the enzyme is related to the well-characterized family of animal secretory PLA2s and , to our knowledge , is the first plant enzyme of this type to be described .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
