| 45 matches found in 8 documents. Search time: 1.027 seconds. |
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Score: 13.00 |
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| Title: A genetic and physical map of the region containing PLASTOCHRON1 , a heterochronic gene , in rice ( Oryza sativa L ) .
| | Author: Ahn O Miyoshi K Itoh JI Nagato Y Kurata N | | Journal: Citation: V : 105 ( 5 ) P : 654-659 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12582477 Accession (PMID): 12582477 | | Abstract: The rice heterochronic gene plastochron1 , pla1 , shows shorter plastochron and ectopic expression of the vegetative program during the rice reproductive phase resulting in aberrant panicle formation .
A genetic and physical map was constructed to isolate the causal gene for the pla1 syndrome .
Small-scale mapping was carried out to determine the approximate map position of the pla1 locus , and then a high-resolution genetic map was made for pla1-1 , one of the pla1 alleles , using an F ( 2 ) population comprising 578 pla1-1 homozygous plants .
In a high-resolution genetic map , the pla1-1 locus was found to map between RFLP markers C961 and R1738A on chromosome 10 , within a 3 . 6-cM genetic distance .
A physical map encompassing the pla1-1 locus was constructed by overlapping Bacterial Artificial Chromosome ( BAC ) clones through chromosome walking .
PCR-based RFLP markers from BAC-end clones were developed and mapped relative to the pla1 locus .
Physical map construction using BAC clones indicated that a BAC clone , B44A10 ( 167-kb ) , contained the pla1 locus within 74-kb corresponding to a 0 . 52-cM genetic distance .
Gene prediction of 74-kb region carrying the pla1 locus suggested several candidate genes for the pla1 gene .
Identification of a candidate gene for pla1 will be made by sequence analysis of allele variation and cDNA screening . | Matching Sentences:
[ Sen. 3, subscore: 4.00 ]: Small-scale mapping was carried out to determine the approximate map position of the pla1 locus , and then a high-resolution genetic map was made for pla1-1 , one of the pla1 alleles , using an F ( 2 ) population comprising 578 pla1-1 homozygous plants .
[ Sen. 8, subscore: 2.00 ]: Gene prediction of 74-kb region carrying the pla1 locus suggested several candidate genes for the pla1 gene .
[ Sen. 1, subscore: 1.00 ]: The rice heterochronic gene plastochron1 , pla1 , shows shorter plastochron and ectopic expression of the vegetative program during the rice reproductive phase resulting in aberrant panicle formation .
[ Sen. 2, subscore: 1.00 ]: A genetic and physical map was constructed to isolate the causal gene for the pla1 syndrome .
[ Sen. 4, subscore: 1.00 ]: In a high-resolution genetic map , the pla1-1 locus was found to map between RFLP markers C961 and R1738A on chromosome 10 , within a 3 . 6-cM genetic distance .
[ Sen. 5, subscore: 1.00 ]: A physical map encompassing the pla1-1 locus was constructed by overlapping Bacterial Artificial Chromosome ( BAC ) clones through chromosome walking .
[ Sen. 6, subscore: 1.00 ]: PCR-based RFLP markers from BAC-end clones were developed and mapped relative to the pla1 locus .
[ Sen. 7, subscore: 1.00 ]: Physical map construction using BAC clones indicated that a BAC clone , B44A10 ( 167-kb ) , contained the pla1 locus within 74-kb corresponding to a 0 . 52-cM genetic distance .
[ Sen. 9, subscore: 1.00 ]: Identification of a candidate gene for pla1 will be made by sequence analysis of allele variation and cDNA screening .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
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| Title: Rice PLASTOCHRON genes regulate leaf maturation downstream of the gibberellin signal transduction pathway .
| | Author: Mimura M Nagato Y Itoh J | | Journal: Planta Citation: V : 235 P : 1081-9 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22476293 Accession (PMID): 22476293 | | Abstract: Rice PLASTOCHRON 1 ( PLA1 ) and PLA2 genes regulate leaf maturation and plastochron , and their loss-of-function mutants exhibit small organs and rapid leaf emergence .
They encode a cytochrome P450 protein CYP78A11 and an RNA-binding protein , respectively .
Their homologs in Arabidopsis and maize are also associated with plant development/organ size .
Despite the importance of PLA genes in plant development , their molecular functions remain unknown .
Here , we investigated how PLA1 and PLA2 genes are related to phytohormones .
We found that gibberellin ( GA ) is the major phytohormone that promotes PLA1 and PLA2 expression .
GA induced PLA1 and PLA2 expression , and conversely the GA-inhibitor uniconazole suppressed PLA1 and PLA2 expression .
In pla1-4 and pla2-1 seedlings , expression levels of GA biosynthesis genes and the signal transduction gene were similar to those in wild-type seedlings .
GA treatment slightly down-regulated the GA biosynthesis gene GA20ox2 and up-regulated the GA-catabolizing gene GA2ox4 , whereas the GA biosynthesis inhibitor uniconazole up-regulated GA20ox2 and down-regulated GA2ox4 both in wild-type and pla mutants , suggesting that the GA feedback mechanism is not impaired in pla1 and pla2 .
To reveal how GA signal transduction affects the expression of PLA1 and PLA2 , PLA expression in GA-signaling mutants was examined .
In GA-insensitive mutant , gid1 and less-sensitive mutant , Slr1-d1 , PLA1 and PLA2 expression was down-regulated .
On the other hand , the expression levels of PLA1 and PLA2 were highly enhanced in a GA-constitutive-active mutant , slr1-1 , causing ectopic overexpression .
These results indicate that both PLA1 and PLA2 act downstream of the GA signal transduction pathway to regulate leaf development .
| Matching Sentences:
[ Sen. 7, subscore: 2.00 ]: GA induced PLA1 and PLA2 expression , and conversely the GA-inhibitor uniconazole suppressed PLA1 and PLA2 expression .
[ Sen. 1, subscore: 1.00 ]: Rice PLASTOCHRON 1 ( PLA1 ) and PLA2 genes regulate leaf maturation and plastochron , and their loss-of-function mutants exhibit small organs and rapid leaf emergence .
[ Sen. 5, subscore: 1.00 ]: Here , we investigated how PLA1 and PLA2 genes are related to phytohormones .
[ Sen. 6, subscore: 1.00 ]: We found that gibberellin ( GA ) is the major phytohormone that promotes PLA1 and PLA2 expression .
[ Sen. 8, subscore: 1.00 ]: In pla1-4 and pla2-1 seedlings , expression levels of GA biosynthesis genes and the signal transduction gene were similar to those in wild-type seedlings .
[ Sen. 9, subscore: 1.00 ]: GA treatment slightly down-regulated the GA biosynthesis gene GA20ox2 and up-regulated the GA-catabolizing gene GA2ox4 , whereas the GA biosynthesis inhibitor uniconazole up-regulated GA20ox2 and down-regulated GA2ox4 both in wild-type and pla mutants , suggesting that the GA feedback mechanism is not impaired in pla1 and pla2 .
[ Sen. 10, subscore: 1.00 ]: To reveal how GA signal transduction affects the expression of PLA1 and PLA2 , PLA expression in GA-signaling mutants was examined .
[ Sen. 11, subscore: 1.00 ]: In GA-insensitive mutant , gid1 and less-sensitive mutant , Slr1-d1 , PLA1 and PLA2 expression was down-regulated .
[ Sen. 12, subscore: 1.00 ]: On the other hand , the expression levels of PLA1 and PLA2 were highly enhanced in a GA-constitutive-active mutant , slr1-1 , causing ectopic overexpression .
[ Sen. 13, subscore: 1.00 ]: These results indicate that both PLA1 and PLA2 act downstream of the GA signal transduction pathway to regulate leaf development .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 11.00 |
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| Title: A recessive heterochronic mutation , plastochron1 , shortens the plastochron and elongates the vegetative phase in rice | | Author: Itoh JI Hasegawa A Kitano H Nagato Y | | Journal: Citation: V : 10 ( 9 ) P : 1511-22 Year: 1998 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9724697 Accession (PMID): 9724697 | | Abstract: We describe two recessive alleles of a rice heterochronic gene , plastochron1-1 ( pla1-1 ) and pla1-2 , that reduce the length of the plastochron to approximately half that of the wild type .
Because the onset of the reproductive phase in pla1 was not temporally affected , the number of leaves produced in the vegetative phase was nearly twice that produced in the wild type .
Panicle development was severely disturbed in pla1 mutants .
In pla1-1 , many primordia of primary rachis branches were converted into vegetative shoots .
These ectopic shoots repeated the initiation of panicle development and the conversion of primary rachis branches into shoots .
In the weak allele pla1-2 , however , only the basal one or two primordia developed as vegetative shoots , and the remaining primordia developed to produce a truncated panicle .
These results indicate that both vegetative and reproductive programs are expressed simultaneously during the reproductive phase of pla1 ; however , the degree varied depending on the strength of the allele .
Accordingly , pla1 is a heterochronic mutation that extends the vegetative period .
The shoot apical meristem of pla1 was larger than that of the wild type , although the shape was not modified .
An in situ hybridization experiment using the histone H4 gene as a probe revealed that cell divisions are accelerated in the pla1 meristem .
The PLA1 gene is considered to regulate the duration of the vegetative phase by controlling the rate of leaf production in the meristem .
| Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: We describe two recessive alleles of a rice heterochronic gene , plastochron1-1 ( pla1-1 ) and pla1-2 , that reduce the length of the plastochron to approximately half that of the wild type .
[ Sen. 2, subscore: 1.00 ]: Because the onset of the reproductive phase in pla1 was not temporally affected , the number of leaves produced in the vegetative phase was nearly twice that produced in the wild type .
[ Sen. 3, subscore: 1.00 ]: Panicle development was severely disturbed in pla1 mutants .
[ Sen. 4, subscore: 1.00 ]: In pla1-1 , many primordia of primary rachis branches were converted into vegetative shoots .
[ Sen. 6, subscore: 1.00 ]: In the weak allele pla1-2 , however , only the basal one or two primordia developed as vegetative shoots , and the remaining primordia developed to produce a truncated panicle .
[ Sen. 7, subscore: 1.00 ]: These results indicate that both vegetative and reproductive programs are expressed simultaneously during the reproductive phase of pla1 ; however , the degree varied depending on the strength of the allele .
[ Sen. 8, subscore: 1.00 ]: Accordingly , pla1 is a heterochronic mutation that extends the vegetative period .
[ Sen. 9, subscore: 1.00 ]: The shoot apical meristem of pla1 was larger than that of the wild type , although the shape was not modified .
[ Sen. 10, subscore: 1.00 ]: An in situ hybridization experiment using the histone H4 gene as a probe revealed that cell divisions are accelerated in the pla1 meristem .
[ Sen. 11, subscore: 1.00 ]: The PLA1 gene is considered to regulate the duration of the vegetative phase by controlling the rate of leaf production in the meristem .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: PLASTOCHRON1 , a timekeeper of leaf initiation in rice , encodes cytochrome P450 .
| | Author: Miyoshi K Ahn BO Kawakatsu T Ito Y Itoh J Nagato Y Kurata N | | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 101 ( 3 ) P : 875-80 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14711998 Accession (PMID): 14711998 | | Abstract: During postembryonic development of higher plants , the shoot apical meristem produces lateral organs in a regular spacing ( phyllotaxy ) and a regular timing ( plastochron ) .
Molecular analysis of mutants associated with phyllotaxy and plastochron would greatly increase understanding of the developmental mechanism of plant architecture because phyllotaxy and plastochron are fundamental regulators of plant architecture . pla1 of rice is not only a plastochron mutant showing rapid leaf initiation without affecting phyllotaxy , but also a heterochronic mutant showing ectopic shoot formation in the reproductive phase .
Thus , pla1 provides a tool for analyzing the molecular basis of temporal regulation in leaf development .
In this work , we isolated the PLA1 gene by map-based cloning .
The identified PLA1 gene encodes a cytochrome P450 , CYP78A11 , which potentially catalyzes substances controlling plant development .
PLA1 is expressed in developing leaf primordia , bracts of the panicle , and elongating internodes , but not in the shoot apical meristem .
The expression pattern and mutant phenotype suggest that the PLA1 gene acting in developing leaf primordia affects the timing of successive leaf initiation and the termination of vegetative growth . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Molecular analysis of mutants associated with phyllotaxy and plastochron would greatly increase understanding of the developmental mechanism of plant architecture because phyllotaxy and plastochron are fundamental regulators of plant architecture . pla1 of rice is not only a plastochron mutant showing rapid leaf initiation without affecting phyllotaxy , but also a heterochronic mutant showing ectopic shoot formation in the reproductive phase .
[ Sen. 3, subscore: 1.00 ]: Thus , pla1 provides a tool for analyzing the molecular basis of temporal regulation in leaf development .
[ Sen. 4, subscore: 1.00 ]: In this work , we isolated the PLA1 gene by map-based cloning .
[ Sen. 5, subscore: 1.00 ]: The identified PLA1 gene encodes a cytochrome P450 , CYP78A11 , which potentially catalyzes substances controlling plant development .
[ Sen. 6, subscore: 1.00 ]: PLA1 is expressed in developing leaf primordia , bracts of the panicle , and elongating internodes , but not in the shoot apical meristem .
[ Sen. 7, subscore: 1.00 ]: The expression pattern and mutant phenotype suggest that the PLA1 gene acting in developing leaf primordia affects the timing of successive leaf initiation and the termination of vegetative growth .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: NECK LEAF 1 , a GATA type transcription factor , modulates organogenesis by regulating the expression of multiple regulatory genes during reproductive development in rice .
| | Author: Wang L Yin H Qian Q Yang J Huang C Hu X Luo D | | Journal: Cell Res Citation: V : 19 P : 598-611 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19337211 Accession (PMID): 19337211 | | Abstract: In the monocot rice species Oryza sativa L , one of the most striking morphological processes during reproductive development is the concurrence of panicle development with the sequential elongation of upper internodes ( UPIs ) .
To elucidate the underlying molecular mechanisms , we cloned the rice gene NECK LEAF 1 ( NL1 ) , which when mutated results in delays in flowering time , smaller panicles with overgrown bracts and abnormal UPI elongation patterns .
The NL1 gene encodes a GATA-type transcription factor with a single zinc finger domain , and its transcripts are detected predominantly in the bract primordia , which normally degenerate in the wild-type plants .
Overexpression of NL1 in transgenic plants often gives rise to severe growth retardation , less vegetative phytomers and smaller leaves , suggesting that NL1 plays an important role in organ differentiation .
A novel mutant allele of PLASTOCHRON1 ( PLA1 ) , a gene known to play a key role in regulating leaf initiation , was identified in this study .
Genetic analysis demonstrated an interaction between nl1 and pla1 , with NL1 acting upstream of PLA1 .
The expression level and spatial pattern of PLA1 were found to be altered in the nl1 mutant .
Furthermore , the expression of two regulators of flowering , Hd3a and OsMADS1 , was also affected in the nl1 mutant .
On the basis of these findings , we propose that NL1 is an intrinsic factor that modulates and coordinates organogenesis through regulating the expression of PLA1 and other regulatory genes during reproductive development in rice .
| Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Genetic analysis demonstrated an interaction between nl1 and pla1 , with NL1 acting upstream of PLA1 .
[ Sen. 5, subscore: 1.00 ]: A novel mutant allele of PLASTOCHRON1 ( PLA1 ) , a gene known to play a key role in regulating leaf initiation , was identified in this study .
[ Sen. 7, subscore: 1.00 ]: The expression level and spatial pattern of PLA1 were found to be altered in the nl1 mutant .
[ Sen. 9, subscore: 1.00 ]: On the basis of these findings , we propose that NL1 is an intrinsic factor that modulates and coordinates organogenesis through regulating the expression of PLA1 and other regulatory genes during reproductive development in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
