| 7 matches found in 4 documents. Search time: 0.094 seconds. |
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Score: 6.00 |
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| Title: Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging .
| | Author: Takahashi A Hayashi N Miyao A Hirochika H | | Journal: BMC Plant Biol Citation: V : 10 P : 175 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20707904 Accession (PMID): 20707904 | | Abstract: BACKGROUND : R gene-mediated resistance is one of the most effective mechanisms of immunity against pathogens in plants .
To date some components that regulate the primary steps of plant immunity have been isolated , however , the molecular dissection of defense signaling downstream of the R proteins remains to be completed .
In addition , R genes are known to be highly variable , however , the molecular mechanisms responsible for this variability remain obscure .
RESULTS : To identify novel factors required for R gene-mediated resistance in rice , we used rice insertional mutant lines , induced by the endogenous retrotransposon Tos17 , in a genetic screening involving the rice blast fungus Magnaporthe oryzae .
We inoculated 41 , 119 mutant lines with the fungus using a high throughput procedure , and identified 86 mutant lines with diminished resistance .
A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site ( NBS ) and leucine rich repeat ( LRR ) domain-containing ( NBS-LRR ) protein .
A genetic complementation analysis and a pathogenesis assay demonstrated that this NBS-LRR gene encodes Pish , which confers resistance against races of M oryzae containing avrPish .
The other 14 lines have intact copies of the Pish gene , suggesting that they may contain mutations in the signaling components downstream of Pish .
The genome analysis indicated that Pish and its neighboring three NBS-LRR genes are high similar to one another and are tandemly located .
An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion .
Intriguingly , the insertion sites are not distributed evenly among these four NBS-LRR genes , despite their similarity at the sequence and expression levels .
CONCLUSIONS : In this work we isolated the R gene Pish , and identified several other mutants involved in the signal transduction required for Pish-mediated resistance .
These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice .
Furthermore , our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes .
Based on this finding , a possible mechanism underlying the high variability of R genes is discussed .
| Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: The other 14 lines have intact copies of the Pish gene , suggesting that they may contain mutations in the signaling components downstream of Pish .
[ Sen. 12, subscore: 2.00 ]: CONCLUSIONS : In this work we isolated the R gene Pish , and identified several other mutants involved in the signal transduction required for Pish-mediated resistance .
[ Sen. 7, subscore: 1.00 ]: A genetic complementation analysis and a pathogenesis assay demonstrated that this NBS-LRR gene encodes Pish , which confers resistance against races of M oryzae containing avrPish .
[ Sen. 9, subscore: 1.00 ]: The genome analysis indicated that Pish and its neighboring three NBS-LRR genes are high similar to one another and are tandemly located .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Rice Pti1a negatively regulates RAR1-dependent defense responses .
| | Author: Takahashi A Agrawal GK Yamazaki M Onosato K Miyao A Kawasaki T Shimamoto K Hirochika H | | Journal: Plant Cell Citation: V : 19 P : 2940-51 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17890377 Accession (PMID): 17890377 | | Abstract: Tomato ( Solanum lycopersicum ) Pto encodes a protein kinase that confers resistance to bacterial speck disease .
A second protein kinase , Pti1 , physically interacts with Pto and is involved in Pto-mediated defense signaling .
Pti1-related sequences are highly conserved among diverse plant species , including rice ( Oryza sativa ) , but their functions are largely unknown .
Here , we report the identification of a null mutant for the Pti1 homolog in rice and the functional characterization of Os Pti1a .
The rice pti1a mutant was characterized by spontaneous necrotic lesions on leaves , which was accompanied by a series of defense responses and resistance against a compatible race of Magnaporthe grisea .
Overexpression of Pti1a in rice reduced resistance against an incompatible race of the fungus recognized by a resistance ( R ) protein , Pish .
Plants overexpressing Pti1a were also more susceptible to a compatible race of the bacterial pathogen Xanthomonas oryzae pv oryzae .
These results suggest that Os Pti1a negatively regulates defense signaling for both R gene-mediated and basal resistance .
We also demonstrated that repression of the rice RAR1 gene suppressed defense responses induced in the pti1a mutant , indicating that Pti1a negatively regulates RAR1-dependent defense responses .
Expression of a tomato Pti1 cDNA in the rice pti1a mutant suppressed the mutant phenotypes .
This contrasts strikingly with the previous finding that Sl Pti1 enhances Pto-mediated hypersensitive response ( HR ) induction when expressed in tobacco ( Nicotiana tabacum ) , suggesting that the molecular switch controlling HR downstream of pathogen recognition has evolved differently in rice and tomato .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Overexpression of Pti1a in rice reduced resistance against an incompatible race of the fungus recognized by a resistance ( R ) protein , Pish .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The identification of Pi50 ( t ) , a new member of the rice blast resistance Pi2/Pi9 multigene family .
| | Author: Zhu X Chen S Yang J Zhou S Zeng L Han J Su J Wang L Pan Q | | Journal: Theor Appl Genet Citation: V : 124 P : 1295-304 Year: 2012 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub22270148 Accession (PMID): 22270148 | | Abstract: The deployment of broad-spectrum resistance genes is the most effective and economic means of controlling blast in rice .
The cultivar Er-Ba-Zhan ( EBZ ) is a widely used donor of blast resistance in South China , with many cultivars derived from it displaying broad-spectrum resistance against blast Mapping in a set of recombinant inbred lines bred from the cross between EBZ and the highly blast-susceptible cultivar Liangjiangxintuanheigu ( LTH ) identified in EBZ a blast resistance gene on each of chromosomes 1 ( Pish ) , 6 ( Pi2/Pi9 ) and 12 ( Pita/Pita-2 ) .
The resistance spectrum and race specificity of the allele at Pi2/Pi9 were both different from those present in other known Pi2/Pi9 carriers .
Fine-scale mapping based on a large number of susceptible EBZ x LTH F ( 2 ) and EBZ x LTH BC ( 1 ) F ( 2 ) segregants placed the gene within a 53-kb segment , which includes Pi2/Pi9 .
Sequence comparisons of the LRR motifs of the four functional NBS-LRR genes within Pi2/Pi9 revealed that the EBZ allele is distinct from other known Pi2/Pi9 alleles .
As a result , the gene has been given the designation Pi50 ( t ) .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The cultivar Er-Ba-Zhan ( EBZ ) is a widely used donor of blast resistance in South China , with many cultivars derived from it displaying broad-spectrum resistance against blast Mapping in a set of recombinant inbred lines bred from the cross between EBZ and the highly blast-susceptible cultivar Liangjiangxintuanheigu ( LTH ) identified in EBZ a blast resistance gene on each of chromosomes 1 ( Pish ) , 6 ( Pi2/Pi9 ) and 12 ( Pita/Pita-2 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging .
| | Author: Takahashi A Hayashi N Miyao A Hirochika H | | Journal: BMC Plant Biol Citation: V : 10 P : 175 Year: 2010 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub20707904 Accession (PMID): 20707904 | | Abstract: BACKGROUND : R gene-mediated resistance is one of the most effective mechanisms of immunity against pathogens in plants .
To date some components that regulate the primary steps of plant immunity have been isolated , however , the molecular dissection of defense signaling downstream of the R proteins remains to be completed .
In addition , R genes are known to be highly variable , however , the molecular mechanisms responsible for this variability remain obscure .
RESULTS : To identify novel factors required for R gene-mediated resistance in rice , we used rice insertional mutant lines , induced by the endogenous retrotransposon Tos17 , in a genetic screening involving the rice blast fungus Magnaporthe oryzae .
We inoculated 41 , 119 mutant lines with the fungus using a high throughput procedure , and identified 86 mutant lines with diminished resistance .
A genome analysis revealed that 72 of the 86 lines contained mutations in a gene encoding a nucleotide binding site ( NBS ) and leucine rich repeat ( LRR ) domain-containing ( NBS-LRR ) protein .
A genetic complementation analysis and a pathogenesis assay demonstrated that this NBS-LRR gene encodes Pish , which confers resistance against races of M oryzae containing avrPish .
The other 14 lines have intact copies of the Pish gene , suggesting that they may contain mutations in the signaling components downstream of Pish .
The genome analysis indicated that Pish and its neighboring three NBS-LRR genes are high similar to one another and are tandemly located .
An in silico analysis of a Tos17 flanking sequence database revealed that this region is a "hot spot" for insertion .
Intriguingly , the insertion sites are not distributed evenly among these four NBS-LRR genes , despite their similarity at the sequence and expression levels .
CONCLUSIONS : In this work we isolated the R gene Pish , and identified several other mutants involved in the signal transduction required for Pish-mediated resistance .
These results indicate that our genetic approach is efficient and useful for unveiling novel aspects of defense signaling in rice .
Furthermore , our data provide experimental evidence that R gene clusters have the potential to be highly preferred targets for transposable element insertions in plant genomes .
Based on this finding , a possible mechanism underlying the high variability of R genes is discussed .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
