Score: 1.00 |
|---|
| Title: Loss of a member of the aquaporin gene family , aqpA affects spore dormancy in Dictyostelium .
| | Author: Mitra BN Yoshino R Morio T Yokoyama M Maeda M Urushihara H Tanaka Y | | Journal: Gene Citation: V : 251 ( 2 ) P : 131-9 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10876090 Accession (PMID): 10876090 | | Abstract: We isolated and characterized a gene from Dictyostelium discoideum , which encodes a protein of 279 amino acids ( 30 . 6kDa ) containing six transmembrane domains with two highly conserved motifs of asparagine-proline-alanine ( NPA ) found in the aquaporin family of water-channel proteins , although the second motif of the protein has been modified into NPV ( asparagine-proline-valine ) .
The deduced amino acid sequence of the gene , which we have named aqpA , is 39% identical to D discoideum WacA , 26% identical to human Aqp5 , 26% identical to Oryza sativa PIP2a , 25% identical to yeast Aqy1 and 24% identical to Ecoli AqpZ .
Southern analyses indicated that aqpA is present as a single copy in the genome .
Northern blot analysis showed that the developmentally regulated 1kb mRNA transcript first appears at the tight mound stage ( 12h ) , and is abundant in fingers ( 16h ) and late culminants ( 20h ) .
In-situ hybridization of slugs revealed that aqpA mRNA accumulated in cells of the prespore region but not in those of the prestalk region .
Disruption of aqpA by homologous recombination did not significantly affect growth or developmental morphogenesis .
Although mutant spores were viable , when assayed soon after encapsulation , they became permeable to propidium iodide and lost viability after a week on the top of a fruiting body .
Thus , AqpA is essential to maintain spore dormancy perhaps through the regulation of water flow . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The deduced amino acid sequence of the gene , which we have named aqpA , is 39% identical to D discoideum WacA , 26% identical to human Aqp5 , 26% identical to Oryza sativa PIP2a , 25% identical to yeast Aqy1 and 24% identical to Ecoli AqpZ .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: cDNA-AFLP analysis of inducible gene expression in rice seminal root tips under a water deficit .
| | Author: Yang L Zheng B Mao C Yi K Liu F Wu Y Tao Q Wu P | | Journal: Gene Citation: V : 314 ( ) P : 141-8 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14527726 Accession (PMID): 14527726 | | Abstract: The seminal roots of an upland rice variety , Azucena , showed accelerated elongation in response to a water deficit .
The elongation of cortical cells in the elongation zone is rapidly stimulated within 16 h by the water deficit . cDNA-AFLP analysis was used to examine gene expression in seminal root tips at four time points ( 4 , 16 , 48 and 72 h ) during the water deficit .
One hundred and six unique genes induced by the water deficit were obtained .
The expression patterns of these genes were confirmed by Northern blot analysis based on 21 selected genes representing different patterns .
The 106 upregulated genes were composed of 60 genes of known function , 28 genes of unknown function and 18 novel genes .
Sixty genes of known functions were involved in transport facilitation , metabolism and energy , stress and defense-related proteins , cellular organization and cell-wall biogenesis , signal transduction , expression regulator and transposable element , suggesting that seminal root tips undergo a complex adaptive process in response to the water deficit .
Expression of 22 genes reached a maximum within 16 h of water deficit treatment .
These included aquaporin ( PIP2a ) , 9-cis-epoxycarotenoid dioxygenase ( NCED1 ) and a negative regulator of gibberellin signal transduction ( SPY ) ; eight other genes participated in cell wall loosening or vesicle traffic . | Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: These included aquaporin ( PIP2a ) , 9-cis-epoxycarotenoid dioxygenase ( NCED1 ) and a negative regulator of gibberellin signal transduction ( SPY ) ; eight other genes participated in cell wall loosening or vesicle traffic .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
