| 4 matches found in 4 documents. Search time: 0.078 seconds. |
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| Title: Expression of two PIP genes in rapidly growing internodes of rice is not primarily controlled by meristem activity or cell expansion .
| | Author: Malz S Sauter M | | Journal: Plant Mol . Biol . Citation: V : 40 ( 6 ) P : 985-95 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10527423 Accession (PMID): 10527423 | | Abstract: Membrane intrinsic proteins facilitate movement of small molecules often times functioning as water channels .
We have identified two genes from rice which encode proteins with characteristic features of plasma membrane intrinsic proteins ( PIP ) .
They possess six membrane-spanning domains , an NPA repeat , overall high sequence homologies and characteristic C and N-terminal hallmark motifs which allowed assignment of OsPIP1a to the PIP1 subfamily and of OsPIP2a to the PIP2 subfamily .
OsPIP1a and OsPIP2a showed similar but not identical expression patterns .
The two genes were expressed at higher levels in seedlings than in adult plants and expression in the primary root was regulated by light .
In internodes of deepwater rice plants which were induced to grow rapidly by submergence , transcript levels were slightly induced in the intercalary meristem ( IM ) and slightly reduced in the elongation zone ( EZ ) after 18 h .
In internodes of GA-induced excised stem sections transcript levels transiently declined in the IM and EZ after 1 h and subsequently recovered to elevated levels after 18 h .
GA also induced OsPIP expression in non-growing it issue after 18 h .
In the IM of submergence-induced stem sections transcript levels remained constitutive .
The different growth-promoting treatments showed no direct correlation between growth rate and OsPIP gene expression in dividing or expanding cells .
In fact , treatment of excised stem sections with ABA or drought stress induced similar changes in OsPIP expression in the growing zone during the first 6 h as GA did .
We conclude that regulation of OsPIP1a and OsPIP2a expression is not primarily controlled by growth .
GA-induced growth may however change the water status of cells which in turn results in altered PIP abundance . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: They possess six membrane-spanning domains , an NPA repeat , overall high sequence homologies and characteristic C and N-terminal hallmark motifs which allowed assignment of OsPIP1a to the PIP1 subfamily and of OsPIP2a to the PIP2 subfamily .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Molecular cloning of a novel water channel from rice : its products expression in Xenopus oocytes and involvement in chilling tolerance .
| | Author: Li L Li S Tao Y Kitagawa Y | | Journal: Citation: V : 154 ( 1 ) P : 43-51 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10725557 Accession (PMID): 10725557 | | Abstract: Water channel proteins , aquaporins , play a fundamental role in transmembrane water movements in plants .
We isolated rice cDNA , rwc1 , by screening a rice ( Oryza sativa cv .
Josaeng Tongil ) cDNA library using a conserved motif of aquaporins .
Like other aquaporin genes , rwc1 encodes a 290-residue protein with six putative transmembrane domains .
The derived amino acid sequence of RWC1 shows high homology with PIP1 ( plasma membrane intrinsic protein 1 ) subfamily members , which suggest it is localized in the plasma membrane .
Injection of its cRNA into Xenopus oocytes increased the osmotic water permeability of the oocytes 2-3 times .
Northern analysis showed that rice aquaporin genes are expressed in rice seedling leaves and roots , but that it disappeared from the root 6 h after osmotic stress began and that the transcript level remained low for about 24 h , then recovered .
The time course of rice aquaporin gene-expression under osmotic stress was correlated with time course of turgor transition in plant .
On the other hand , the levels of rice aquaporin gene-transcripts in leaves under chilling and recovery temperature depend on the pretreatment of mannitol for short time .
This variation of the transcripts shown that rice aquaporin genes may play an important role in response to water stress-induced chilling tolerance . | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: The derived amino acid sequence of RWC1 shows high homology with PIP1 ( plasma membrane intrinsic protein 1 ) subfamily members , which suggest it is localized in the plasma membrane .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: Role of the aquaporin PIP1 subfamily in the chilling tolerance of rice .
| | Author: Matsumoto T Lian HL Su WA Tanaka D Liu C Iwasaki I Kitagawa Y | | Journal: Plant Cell Physiol Citation: V : 50 P : 216-29 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19098326 Accession (PMID): 19098326 | | Abstract: Although an association between chilling tolerance and aquaporins has been reported , the exact mechanisms involved in this relationship remain unclear .
We compared the expression profiles of aquaporin genes between a chilling-tolerant and a low temperature-sensitive rice variety using real-time PCR and identified seven genes that closely correlated with chilling tolerance .
Chemical treatment experiments , by which rice plants were induced to lose their chilling tolerance , implicated the PIP1 ( plasma membrane intrinsic protein 1 ) subfamily member genes in chilling tolerance .
Of these members , changes in expression of the OsPIP1 ; 3 gene suggested this to be the most closely related to chilling tolerance .
Although OsPIP1 ; 3 showed a much lower water permeability than members of the OsPIP2 family , OsPIP1 ; 3 enhanced the water permeability of OsPIP2 ; 2 and OsPIP2 ; 4 when co-expressed with either of these proteins in oocytes .
Transgenic rice plants ( OE1 ) overexpressing OsPIP1 ; 3 showed an enhanced level of chilling tolerance and the ability to maintain high OsPIP1 ; 3 expression levels under low temperature treatment , similar to that of chilling-tolerant rice plants .
We assume that OsPIP1 ; 3 , constitutively overexpressed in the leaf and root of transgenic OE1 plants , interacts with members of the OsPIP2 subfamily , thereby improving the plants water balance under low temperatures and resulting in the observed chilling tolerance of the plants .
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[ Sen. 3, subscore: 1.00 ]: Chemical treatment experiments , by which rice plants were induced to lose their chilling tolerance , implicated the PIP1 ( plasma membrane intrinsic protein 1 ) subfamily member genes in chilling tolerance .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Role of the aquaporin PIP1 subfamily in the chilling tolerance of rice .
| | Author: Matsumoto T Lian HL Su WA Tanaka D Liu C Iwasaki I Kitagawa Y | | Journal: Plant Cell Physiol Citation: V : 50 P : 216-29 Year: 2009 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub19098326 Accession (PMID): 19098326 | | Abstract: Although an association between chilling tolerance and aquaporins has been reported , the exact mechanisms involved in this relationship remain unclear .
We compared the expression profiles of aquaporin genes between a chilling-tolerant and a low temperature-sensitive rice variety using real-time PCR and identified seven genes that closely correlated with chilling tolerance .
Chemical treatment experiments , by which rice plants were induced to lose their chilling tolerance , implicated the PIP1 ( plasma membrane intrinsic protein 1 ) subfamily member genes in chilling tolerance .
Of these members , changes in expression of the OsPIP1 ; 3 gene suggested this to be the most closely related to chilling tolerance .
Although OsPIP1 ; 3 showed a much lower water permeability than members of the OsPIP2 family , OsPIP1 ; 3 enhanced the water permeability of OsPIP2 ; 2 and OsPIP2 ; 4 when co-expressed with either of these proteins in oocytes .
Transgenic rice plants ( OE1 ) overexpressing OsPIP1 ; 3 showed an enhanced level of chilling tolerance and the ability to maintain high OsPIP1 ; 3 expression levels under low temperature treatment , similar to that of chilling-tolerant rice plants .
We assume that OsPIP1 ; 3 , constitutively overexpressed in the leaf and root of transgenic OE1 plants , interacts with members of the OsPIP2 subfamily , thereby improving the plants water balance under low temperatures and resulting in the observed chilling tolerance of the plants .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Role of the aquaporin PIP1 subfamily in the chilling tolerance of rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
