Score: 6.00 | Title: The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes .
| Author: Yuan B Zhai C Wang W Zeng X Xu X Hu H Lin F Wang L Pan Q | Journal: Theor Appl Genet Citation: V : 122 P : 1017-28 Year: 2011 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub21153625 Accession (PMID): 21153625 | Abstract: The blast resistance gene Pik-p , mapping to the Pik locus on the long arm of rice chromosome 11 , was isolated by map-based in silico cloning .
Four NBS-LRR genes are present in the target region of cv .
Nipponbare , and a presence/absence analysis in the Pik-p carrier cv .
K60 excluded two of these as candidates for Pik-p .
The other two candidates ( KP3 and KP4 ) were expressed in cv .
K60 .
A loss-of-function experiment by RNAi showed that both KP3 and KP4 are required for Pik-p function , while a gain-of-function experiment by complementation test revealed that neither KP3 nor KP4 on their own can impart resistance , but that resistance was expressed when both were introduced simultaneously .
Both Pikp-1 ( KP3 ) and Pikp-2 ( KP4 ) encode coiled-coil NBS-LRR proteins and share , respectively , 95 and 99% peptide identity with the two alleles , Pikm1-TS and Pikm2-TS .
The Pikp-1 and Pikp-2 sequences share only limited homology .
Their sequence allowed Pik-p to be distinguished from Pik , Pik-s , Pik-m and Pik-h .
Both Pikp-1 and Pikp-2 were constitutively expressed in cv .
K60 and only marginally induced by blast infection .
| Matching Sentences: [ Sen. 8, subscore: 2.00 ]: Both Pikp-1 ( KP3 ) and Pikp-2 ( KP4 ) encode coiled-coil NBS-LRR proteins and share , respectively , 95 and 99% peptide identity with the two alleles , Pikm1-TS and Pikm2-TS . [ Sen. 9, subscore: 2.00 ]: The Pikp-1 and Pikp-2 sequences share only limited homology . [ Sen. 11, subscore: 2.00 ]: Both Pikp-1 and Pikp-2 were constitutively expressed in cv .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |