| 25 matches found in 12 documents. Search time: 0.218 seconds. |
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Score: 13.00 |
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| Title: Characterization and fine mapping of the rice blast resistance gene Pia .
| | Author: Zeng X Yang X Zhao Z Lin F Wang L Pan Q | | Journal: Sci China Life Sci Citation: V : 54 P : 372-8 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21373980 Accession (PMID): 21373980 | | Abstract: Blast , caused by Magnaporthe oryzae , is one of the most widespread and destructive diseases of rice .
Breeding durable resistant cultivars ( cvs ) can be achieved by pyramiding of various resistance ( R ) genes .
Pia , carried by cv .
Aichi Asahi , was evaluated against 612 isolates of M oryzae collected from 10 Chinese provinces .
The Pia gene expresses weak resistance in all the provinces except for Jiangsu .
Genomic position-ready marker-based linkage analysis was carried out in a mapping population consisting of 800 F ( 2 ) plants derived from a cross of Aichi AsahixKasalath .
The locus was defined in an interval of approximately 90 kb , flanked by markers A16 and A21 .
Four candidate genes ( Pia-1 , Pia-2 , Pia-3 , and Pia-4 ) , all having the R gene conserved structure , were predicted in the interval using the cv .
Nipponbare genomic sequence .
Four candidate resistance gene ( CRG ) markers ( A17 , A25 , A26 , and A27 ) , derived from the four candidates , were subjected to genotyping with the recombinants detected at the flanking markers .
The first three markers completely co-segregated with the Pia locus , and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers , indicating that the fourth candidate gene , Pia-4 , could be excluded .
Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 ( Pia-3 ) perfectly matched the R gene genotype of Pia , indicating that Pia-3 is the strongest candidate gene for Pia .
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[ Sen. 8, subscore: 4.00 ]: Four candidate genes ( Pia-1 , Pia-2 , Pia-3 , and Pia-4 ) , all having the R gene conserved structure , were predicted in the interval using the cv .
[ Sen. 12, subscore: 4.00 ]: Co-segregation marker-based genotyping of the three sets of differentials with known R gene genotypes revealed that the genotype of A26 ( Pia-3 ) perfectly matched the R gene genotype of Pia , indicating that Pia-3 is the strongest candidate gene for Pia .
[ Sen. 11, subscore: 3.00 ]: The first three markers completely co-segregated with the Pia locus , and the fourth was absent in the Aichi Asahi genome and disordered with the Pia locus and its flanking markers , indicating that the fourth candidate gene , Pia-4 , could be excluded .
[ Sen. 3, subscore: 1.00 ]: Pia , carried by cv .
[ Sen. 5, subscore: 1.00 ]: The Pia gene expresses weak resistance in all the provinces except for Jiangsu .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 12.00 |
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| Title: A multifaceted genomics approach allows the isolation of the rice Pia-blast resistance gene consisting of two adjacent NBS-LRR protein genes .
| | Author: Okuyama Y Kanzaki H Abe A Yoshida K Tamiru M Saitoh H Fujibe T Matsumura H Shenton M Galam DC Undan J Ito A Sone T Terauchi R | | Journal: Plant J Citation: V : 66 P : 467-79 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21251109 Accession (PMID): 21251109 | | Abstract: The Oryza sativa ( rice ) resistance gene Pia confers resistance to the blast fungus Magnaporthe oryzae carrying the AVR-Pia avirulence gene .
To clone Pia , we employed a multifaceted genomics approach .
First , we selected 12 R-gene analog ( RGA ) genes encoding nucleotide binding site-leucine rich repeats ( NBS-LRRs ) proteins from a region on chromosome 11 that shows linkage to Pia .
By using seven rice accessions , we examined the association between Pia phenotypes and DNA polymorphisms in the 10 genes , which revealed three genes ( Os11gRGA3-Os11gRGA5 ) exhibiting a perfect association with the Pia phenotypes .
We also screened ethyl methane sulfonate ( EMS ) -treated mutant lines of the rice cultivar Sasanishiki harboring Pia , and isolated two mutants that lost the Pia phenotype .
DNA sequencing of Os11gRGA3-Os11gRGA5 from the two mutant lines identified independent mutations of major effects in Os11gRGA4 .
The wild-type Sasanishiki allele of Os11gRGA4 ( SasRGA4 ) complemented Pia function in both mutants , suggesting that SasRGA4 is necessary for Pia function .
However , when the rice cultivar Himenomochi lacking Pia was transfected with SasRGA4 , the Pia phenotype was not recovered .
An additional complementation study revealed that the two NBS-LRR-type R genes , SasRGA4 and SasRGA5 , that are located next to each other and oriented in the opposite direction are necessary for Pia function .
A population genetics analysis of SasRGA4 and SasRGA5 suggests that the two genes are under long-term balancing selection .
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[ Sen. 4, subscore: 2.00 ]: By using seven rice accessions , we examined the association between Pia phenotypes and DNA polymorphisms in the 10 genes , which revealed three genes ( Os11gRGA3-Os11gRGA5 ) exhibiting a perfect association with the Pia phenotypes .
[ Sen. 5, subscore: 2.00 ]: We also screened ethyl methane sulfonate ( EMS ) -treated mutant lines of the rice cultivar Sasanishiki harboring Pia , and isolated two mutants that lost the Pia phenotype .
[ Sen. 7, subscore: 2.00 ]: The wild-type Sasanishiki allele of Os11gRGA4 ( SasRGA4 ) complemented Pia function in both mutants , suggesting that SasRGA4 is necessary for Pia function .
[ Sen. 8, subscore: 2.00 ]: However , when the rice cultivar Himenomochi lacking Pia was transfected with SasRGA4 , the Pia phenotype was not recovered .
[ Sen. 1, subscore: 1.00 ]: The Oryza sativa ( rice ) resistance gene Pia confers resistance to the blast fungus Magnaporthe oryzae carrying the AVR-Pia avirulence gene .
[ Sen. 2, subscore: 1.00 ]: To clone Pia , we employed a multifaceted genomics approach .
[ Sen. 3, subscore: 1.00 ]: First , we selected 12 R-gene analog ( RGA ) genes encoding nucleotide binding site-leucine rich repeats ( NBS-LRRs ) proteins from a region on chromosome 11 that shows linkage to Pia .
[ Sen. 9, subscore: 1.00 ]: An additional complementation study revealed that the two NBS-LRR-type R genes , SasRGA4 and SasRGA5 , that are located next to each other and oriented in the opposite direction are necessary for Pia function .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Rice ESTs with disease-resistance gene or defense-response gene-like sequences mapped to regions containing major resistance genes or QTLs .
| | Author: Wang Z Taramino G Yang D Liu G Tingey SV Miao GH Wang GL .
| | Journal: Mol . Genet . Genomics Citation: V : 265 ( 2 ) P : 302-10 Year: 2001 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11361341 Accession (PMID): 11361341 | | Abstract: The chromosomal locations of 109 rice expressed sequence tags ( ESTs ) in the rice genome were determined using a doubled haploid mapping population .
These ESTs show high similarity to disease resistance genes or to defense response genes .
Nine of the ESTs were mapped to three regions that contain genetically defined resistance genes on chromosomes 6 and 11 .
Clustering of the ESTs in the rice genome was observed at several chromosomal regions .
Some of the clusters were located in regions where quantitative trait loci ( QTL ) associated with partial resistance to rice blast , bacterial blight and sheath blight are known to lie .
Three ESTs that were mapped to the regions containing blast resistance genes Pi2 and Pia were chosen for Northern analysis after inoculation of plants with the blast fungus .
Two of them , which code for a receptor-like kinase and a putative membrane channel protein , respectively , and were mapped to the Pi2 locus , were induced by rice blast infection as early as 4 h after inoculation .
Transcription of another EST , which codes for a homolog of a putative human tumor suppressor and was mapped to the region containing Pia , was repressed after blast infection .
These findings demonstrate that the candidate-gene approach is an efficient way of mapping resistance genes or resistance QTLs in rice . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Three ESTs that were mapped to the regions containing blast resistance genes Pi2 and Pia were chosen for Northern analysis after inoculation of plants with the blast fungus .
[ Sen. 8, subscore: 1.00 ]: Transcription of another EST , which codes for a homolog of a putative human tumor suppressor and was mapped to the region containing Pia , was repressed after blast infection .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Biochemical effects of clomazone herbicide on piava ( Leporinus obtusidens ) .
| | Author: Miron Ddos S Pretto A Crestani M Glusczak L Schetinger MR Loro VL Morsch VM | | Journal: Chemosphere Citation: V : 74 P : 1-5 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18990427 Accession (PMID): 18990427 | | Abstract: This study aims to verify the effects of the clomazone concentration used in rice fields on acetylcholinesterase ( AChE ) , thiobarbituric acid reactive substances ( TBARS ) , protein carbonyl and catalase activity in it issues of piava ( Leporinus obtusidens ) .
LC ( 50 ) -96h was 5 . 0 mg L ( -1 ) and the fish were exposed to 1/10 of LC ( 50 ) -96 h : 0 . 5 mg L ( -1 ) of clomazone for 96 and 192h .
The same parameters were also assayed after a recovery period of 192 h in clean water .
AChE activity was reduced only in the brain and heart of fish exposed for 96 h .
AChE activity was decreased in the brain , muscle and heart it issues after 192 h of exposure .
After 192 h of recovery period , AChE activity remained diminished in brain and muscle and showed a decrease in eye .
However , after 192 h of recovery , AChE activity in heart was recovered .
Fish showed increased TBARS levels in brain at all experimental periods .
TBARS levels decreased in liver and muscle it issues after 192 h of exposure .
The increase in muscle TBARS persisted in fish transferred to clean water .
Protein carbonyl in the liver was increased in all periods studied including the recovery period .
Catalase activity was reduced during all periods .
The present study demonstrates the occurrence of disorders in AChE , TBARS , protein carbonyl and catalase activity in piava .
The results also show changes in fish after exposure to an environmentally relevant concentration of clomazone .
Most effects observed persisted after the recovery period .
Thus , these parameters may be used to monitor clomazone toxicity in fish .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: This study aims to verify the effects of the clomazone concentration used in rice fields on acetylcholinesterase ( AChE ) , thiobarbituric acid reactive substances ( TBARS ) , protein carbonyl and catalase activity in it issues of piava ( Leporinus obtusidens ) .
[ Sen. 13, subscore: 1.00 ]: The present study demonstrates the occurrence of disorders in AChE , TBARS , protein carbonyl and catalase activity in piava .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: [ Rapid establishment of suspension cell lines in japonica rice ] | | Author: Zhang H Yang F Gao M Xu P Zhang Q Xu J Wu X | | Journal: Sheng Wu Gong Cheng Xue Bao Citation: V : 25 P : 424-7 Year: 2009 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub19621585 Accession (PMID): 19621585 | | Abstract: With Jingkang No5 ( PiA ) , calli of the PiA induced for 10-15 days were transferred into amino acid liquid culture medium , to establish excellent rice suspension cell lines successfully in a relative short time .
The growth characteristics and differentiation conditions of suspension cells were measured at different phases .
Results revealed that the optimal subculture time was 7-10 days , and the cells cultured for 30-120 days had the best differentiation ability ( 57 . 1% ) and regeneration ability ( 20% ) .
This study is promising in further using the suspension cell for genetic transformation and protoplasm isolation .
| Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: With Jingkang No5 ( PiA ) , calli of the PiA induced for 10-15 days were transferred into amino acid liquid culture medium , to establish excellent rice suspension cell lines successfully in a relative short time .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
