| 10 matches found in 4 documents. Search time: 0.201 seconds. |
|---|
|
Score: 5.00 |
|---|
| Title: A novel gene , Pi40 ( t ) , linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice .
| | Author: Jeung JU Kim BR Cho YC Han SS Moon HP Lee YT Jena KK | | Journal: Theor Appl Genet Citation: V : 115 P : 1163-77 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17909744 Accession (PMID): 17909744 | | Abstract: Rice blast disease caused by Magnaporthe grisea is a continuous threat to stable rice production worldwide .
In a modernized agricultural system , the development of varieties with broad-spectrum and durable resistance to blast disease is essential for increased rice production and sustainability .
In this study , a new gene is identified in the introgression line IR65482-4-136-2-2 that has inherited the resistance gene from an EE genome wild Oryza species , O australiensis ( Acc .
100882 ) .
Genetic and molecular analysis localized a major resistance gene , Pi40 ( t ) , on the short arm of chromosome 6 , where four blast resistance genes ( Piz , Piz-5 , Piz-t , and Pi9 ) were also identified , flanked by the markers S2539 and RM3330 .
Through e-Landing , 14 BAC/PAC clones within the 1 . 81-Mb equivalent virtual contig were identified on Rice Pseudomolecule3 .
Highly stringent primer sets designed for 6 NBS-LRR motifs located within PAC clone P0649C11 facilitated high-resolution mapping of the new resistance gene , Pi40 ( t ) .
Following association analysis and detailed haplotyping approaches , a DNA marker , 9871 . T7E2b , was identified to be linked to the Pi40 ( t ) gene at the 70 Kb chromosomal region , and differentiated the Pi40 ( t ) gene from the LTH monogenic differential lines possessing genes Piz , Piz-5 , Piz-t , and Pi-9 .
Pi40 ( t ) was validated using the most virulent isolates of Korea as well as the Philippines , suggesting a broad spectrum for the resistance gene .
Marker-assisted selection ( MAS ) and pathotyping of BC progenies having two japonica cultivar genetic backgrounds further supported the potential of the resistance gene in rice breeding .
Our study based on new gene identification strategies provides insight into novel genetic resources for blast resistance as well as future studies on cloning and functional analysis of a blast resistance gene useful for rice improvement .
| Matching Sentences:
[ Sen. 8, subscore: 2.00 ]: Following association analysis and detailed haplotyping approaches , a DNA marker , 9871 . T7E2b , was identified to be linked to the Pi40 ( t ) gene at the 70 Kb chromosomal region , and differentiated the Pi40 ( t ) gene from the LTH monogenic differential lines possessing genes Piz , Piz-5 , Piz-t , and Pi-9 .
[ Sen. 5, subscore: 1.00 ]: Genetic and molecular analysis localized a major resistance gene , Pi40 ( t ) , on the short arm of chromosome 6 , where four blast resistance genes ( Piz , Piz-5 , Piz-t , and Pi9 ) were also identified , flanked by the markers S2539 and RM3330 .
[ Sen. 7, subscore: 1.00 ]: Highly stringent primer sets designed for 6 NBS-LRR motifs located within PAC clone P0649C11 facilitated high-resolution mapping of the new resistance gene , Pi40 ( t ) .
[ Sen. 9, subscore: 1.00 ]: Pi40 ( t ) was validated using the most virulent isolates of Korea as well as the Philippines , suggesting a broad spectrum for the resistance gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
|---|
| Title: The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines .
| | Author: Suh JP Roh JH Cho YC Han SS Kim YG Jena KK | | Journal: Phytopathology Citation: V : 99 P : 243-50 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19203276 Accession (PMID): 19203276 | | Abstract: Rice blast severely reduces production in both irrigated and water-stressed upland ecosystems of tropical and temperate countries .
Nearly 50 blast resistance genes have been identified and some of those are incorporated into several rice cultivars .
However , most of the resistance genes break down in a few years because of their race specificity and the rapid change in pathogenicity of the blast fungus ( Magnaporthe grisea ) .
The objective of this study was to analyze advanced backcross breeding lines ( ABL ) possessing the gene Pi40 for durable rice blast resistance .
In all , 4 resistant genotypes , 4 japonica cultivars , and 10 monogenic differential rice genotypes with some known resistance genes were bioassayed in the greenhouse using seven sequential plantings and 29 virulent M grisea isolates of Korea .
The genotypes with the Pi40 gene had <3% diseased leaf area , which was significantly below the disease threshold level of 40% considered for durable blast resistance .
Moreover , the genotypes with the Pi40 gene expressed compatibility with only two to three virulent M grisea isolates supporting durability of resistance , in contrast to susceptible cultivars with >50% diseased leaf area and 10 compatible isolates .
Of the 10 known resistance genes tested , Piz-t , Piz-5 , and Pi9 showed differential reactions to the pathogen isolates in seven plantings .
Genotyping of the ABL with 260 simple sequence repeat ( SSR ) markers revealed rapid conversion toward recurrent parent genotypes with fewer donor chromosomal segments ( 5 . 3 to 14 . 5% ) .
Our study based on a sequential testing and background selection of breeding lines with the resistance gene Pi40 provided valuable information for durable blast resistance breeding in rice .
| Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The objective of this study was to analyze advanced backcross breeding lines ( ABL ) possessing the gene Pi40 for durable rice blast resistance .
[ Sen. 6, subscore: 1.00 ]: The genotypes with the Pi40 gene had <3% diseased leaf area , which was significantly below the disease threshold level of 40% considered for durable blast resistance .
[ Sen. 7, subscore: 1.00 ]: Moreover , the genotypes with the Pi40 gene expressed compatibility with only two to three virulent M grisea isolates supporting durability of resistance , in contrast to susceptible cultivars with >50% diseased leaf area and 10 compatible isolates .
[ Sen. 10, subscore: 1.00 ]: Our study based on a sequential testing and background selection of breeding lines with the resistance gene Pi40 provided valuable information for durable blast resistance breeding in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines .
| | Author: Suh JP Roh JH Cho YC Han SS Kim YG Jena KK | | Journal: Phytopathology Citation: V : 99 P : 243-50 Year: 2009 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub19203276 Accession (PMID): 19203276 | | Abstract: Rice blast severely reduces production in both irrigated and water-stressed upland ecosystems of tropical and temperate countries .
Nearly 50 blast resistance genes have been identified and some of those are incorporated into several rice cultivars .
However , most of the resistance genes break down in a few years because of their race specificity and the rapid change in pathogenicity of the blast fungus ( Magnaporthe grisea ) .
The objective of this study was to analyze advanced backcross breeding lines ( ABL ) possessing the gene Pi40 for durable rice blast resistance .
In all , 4 resistant genotypes , 4 japonica cultivars , and 10 monogenic differential rice genotypes with some known resistance genes were bioassayed in the greenhouse using seven sequential plantings and 29 virulent M grisea isolates of Korea .
The genotypes with the Pi40 gene had <3% diseased leaf area , which was significantly below the disease threshold level of 40% considered for durable blast resistance .
Moreover , the genotypes with the Pi40 gene expressed compatibility with only two to three virulent M grisea isolates supporting durability of resistance , in contrast to susceptible cultivars with >50% diseased leaf area and 10 compatible isolates .
Of the 10 known resistance genes tested , Piz-t , Piz-5 , and Pi9 showed differential reactions to the pathogen isolates in seven plantings .
Genotyping of the ABL with 260 simple sequence repeat ( SSR ) markers revealed rapid conversion toward recurrent parent genotypes with fewer donor chromosomal segments ( 5 . 3 to 14 . 5% ) .
Our study based on a sequential testing and background selection of breeding lines with the resistance gene Pi40 provided valuable information for durable blast resistance breeding in rice .
| Matching Sentences:
[ Sen. 1, subscore: 2.00 ]: The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: A novel gene , Pi40 ( t ) , linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice .
| | Author: Jeung JU Kim BR Cho YC Han SS Moon HP Lee YT Jena KK | | Journal: Theor Appl Genet Citation: V : 115 P : 1163-77 Year: 2007 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub17909744 Accession (PMID): 17909744 | | Abstract: Rice blast disease caused by Magnaporthe grisea is a continuous threat to stable rice production worldwide .
In a modernized agricultural system , the development of varieties with broad-spectrum and durable resistance to blast disease is essential for increased rice production and sustainability .
In this study , a new gene is identified in the introgression line IR65482-4-136-2-2 that has inherited the resistance gene from an EE genome wild Oryza species , O australiensis ( Acc .
100882 ) .
Genetic and molecular analysis localized a major resistance gene , Pi40 ( t ) , on the short arm of chromosome 6 , where four blast resistance genes ( Piz , Piz-5 , Piz-t , and Pi9 ) were also identified , flanked by the markers S2539 and RM3330 .
Through e-Landing , 14 BAC/PAC clones within the 1 . 81-Mb equivalent virtual contig were identified on Rice Pseudomolecule3 .
Highly stringent primer sets designed for 6 NBS-LRR motifs located within PAC clone P0649C11 facilitated high-resolution mapping of the new resistance gene , Pi40 ( t ) .
Following association analysis and detailed haplotyping approaches , a DNA marker , 9871 . T7E2b , was identified to be linked to the Pi40 ( t ) gene at the 70 Kb chromosomal region , and differentiated the Pi40 ( t ) gene from the LTH monogenic differential lines possessing genes Piz , Piz-5 , Piz-t , and Pi-9 .
Pi40 ( t ) was validated using the most virulent isolates of Korea as well as the Philippines , suggesting a broad spectrum for the resistance gene .
Marker-assisted selection ( MAS ) and pathotyping of BC progenies having two japonica cultivar genetic backgrounds further supported the potential of the resistance gene in rice breeding .
Our study based on new gene identification strategies provides insight into novel genetic resources for blast resistance as well as future studies on cloning and functional analysis of a blast resistance gene useful for rice improvement .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A novel gene , Pi40 ( t ) , linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
