| 18 matches found in 7 documents. Search time: 0.12 seconds. |
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Score: 6.00 |
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| Title: The in silico map-based cloning of Pi36 , a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus .
| | Author: Liu X Lin F Wang L Pan Q | | Journal: Genetics Citation: V : 176 P : Sep-41 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17507669 Accession (PMID): 17507669 | | Abstract: The indica rice variety Kasalath carries Pi36 , a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8 .
The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance ( R ) gene content of the interval and hence for the identification of candidate gene ( s ) for Pi36 .
Three such sequences , which all had both a nucleotide-binding site and a leucine-rich repeat motif , were present .
The three candidate genes were amplified from the genomic DNA of a number of varieties by long-range PCR , and the resulting amplicons were inserted into pCAMBIA1300 and/or pYLTAC27 vectors to determine sequence polymorphisms correlated to the resistance phenotype and to perform transgenic complementation tests .
Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063 , which allowed the identification of Pi36-3 as the functional gene , with the other two candidates being probable pseudogenes .
The Pi36-encoded protein is composed of 1056 amino acids , with a single substitution event ( Asp to Ser ) at residue 590 associated with the resistant phenotype .
Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
An RT-PCR analysis showed that Pi36 is constitutively expressed in Kasalath .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The indica rice variety Kasalath carries Pi36 , a gene that determines resistance to Chinese isolates of rice blast and that has been located to a 17-kb interval on chromosome 8 .
[ Sen. 2, subscore: 1.00 ]: The genomic sequence of the reference japonica variety Nipponbare was used for an in silico prediction of the resistance ( R ) gene content of the interval and hence for the identification of candidate gene ( s ) for Pi36 .
[ Sen. 5, subscore: 1.00 ]: Constructs containing each candidate gene were transformed into the blast-susceptible variety Q1063 , which allowed the identification of Pi36-3 as the functional gene , with the other two candidates being probable pseudogenes .
[ Sen. 6, subscore: 1.00 ]: The Pi36-encoded protein is composed of 1056 amino acids , with a single substitution event ( Asp to Ser ) at residue 590 associated with the resistant phenotype .
[ Sen. 7, subscore: 1.00 ]: Pi36 is a single-copy gene in rice and is more closely related to the barley powdery mildew resistance genes Mla1 and Mla6 than to the rice blast R genes Pita , Pib , Pi9 , and Piz-t .
[ Sen. 8, subscore: 1.00 ]: An RT-PCR analysis showed that Pi36 is constitutively expressed in Kasalath .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 5.00 |
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| Title: Genetic and physical mapping of Pi36 ( t ) , a novel rice blast resistance gene located on rice chromosome 8 .
| | Author: Liu XQ Wang L Chen S Lin F Pan QH .
| | Journal: Mol . Genet . Genomics Citation: V : 274 ( 4 ) P : 394-401 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16151856 Accession (PMID): 16151856 | | Abstract: Blast resistance in the indica cultivar ( cv . ) Q61 was inherited as a single dominant gene in two F2 populations , F2-1 and F2-2 , derived from crosses between the donor cv . and two susceptible japonica cvs Aichi Asahi and Lijiangxintuanheigu ( LTH ) , respectively .
To rapidly determine the chromosomal location of the resistance ( R ) gene detected in Q61 , random amplified polymorphic DNA ( RAPD ) analysis was performed in the F2-1 population using bulked-segregant analysis ( BSA ) in combination with recessive-class analysis ( RCA ) .
One of the three linked markers identified , BA1126 ( 550 ) , was cloned and sequenced .
The R gene locus was roughly mapped on rice chromosome 8 by comparison of the BA1126 ( 550 ) sequence with rice sequences in the databases ( chromosome landing ) .
To confirm this finding , seven known markers , including four sequence-tagged-site ( STS ) markers and three simple-sequence repeat ( SSR ) markers flanking BA1126 ( 550 ) on chromosome 8 , were subjected to linkage analysis in the two F2 populations .
The locus was mapped to a 5 . 8 cM interval bounded by RM5647 and RM8018 on the short arm of chromosome 8 .
This novel R gene is therefore tentatively designated as Pi36 ( t ) .
For fine mapping of the Pi36 ( t ) locus , five additional markers including one STS marker and four candidate resistance gene ( CRG ) markers were developed in the target region , based on the genomic sequence of the corresponding region of the reference japonica cv .
Nipponbare .
The Pi36 ( t ) locus was finally localized to an interval of about 0 . 6 cM flanked by the markers RM5647 and CRG2 , and co-segregated with the markers CRG3 and CRG4 .
To physically map this locus , the Pi36 ( t ) -linked markers were mapped by electronic hybridization to bacterial artificial chromosome ( BAC ) or P1 artificial chromosome ( PAC ) clones of Nipponbare , and a contig map was constructed in silico through Pairwise BLAST analysis .
The Pi36 ( t ) locus was physically delimited to an interval of about 17 . 0 kb , based on the genomic sequence of Nipponbare . | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: This novel R gene is therefore tentatively designated as Pi36 ( t ) .
[ Sen. 8, subscore: 1.00 ]: For fine mapping of the Pi36 ( t ) locus , five additional markers including one STS marker and four candidate resistance gene ( CRG ) markers were developed in the target region , based on the genomic sequence of the corresponding region of the reference japonica cv .
[ Sen. 10, subscore: 1.00 ]: The Pi36 ( t ) locus was finally localized to an interval of about 0 . 6 cM flanked by the markers RM5647 and CRG2 , and co-segregated with the markers CRG3 and CRG4 .
[ Sen. 11, subscore: 1.00 ]: To physically map this locus , the Pi36 ( t ) -linked markers were mapped by electronic hybridization to bacterial artificial chromosome ( BAC ) or P1 artificial chromosome ( PAC ) clones of Nipponbare , and a contig map was constructed in silico through Pairwise BLAST analysis .
[ Sen. 12, subscore: 1.00 ]: The Pi36 ( t ) locus was physically delimited to an interval of about 17 . 0 kb , based on the genomic sequence of Nipponbare .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Study on the interaction between methyl jasmonate and the coiled-coil domain of rice blast resistance protein Pi36 by spectroscopic methods .
| | Author: Liu XQ Zhang D Zhang XM Wang CT Liu XQ Tan YP Wu YH | | Journal: Spectrochim Acta A Mol Biomol Spectrosc Citation: V : 88 P : 72-6 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22196797 Accession (PMID): 22196797 | | Abstract: Interaction between the coiled-coil domain of rice blast resistance protein Pi36 and methyl-jasmonate ( MeJA ) was studied by fluorescence and UV-vis spectroscopic techniques .
The quenching mechanism of fluorescence of MeJA by this domain was discussed to be a static quenching procedure .
Fluorescence quenching was explored to measure the number of binding sites n and apparent binding constants K The thermodynamics parameters DeltaH , DeltaG , DeltaS were also calculated .
The results indicate the binding reaction was not entropy-driven but enthalpy-driven , and hydrophobic binding played major role in the interaction .
The binding sites of MeJA with the coiled-coil structural domain of rice blast resistance protein Pi36 were found to approach the microenvironment of both Tyr and Trp by the synchronous fluorescence spectrometry .
The distance r between donor ( the coiled-coil domain of rice blast resistance protein Pi36 ) and acceptor ( MeJA ) was obtained according to Forster theory of non-radioactive energy transfer .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Interaction between the coiled-coil domain of rice blast resistance protein Pi36 and methyl-jasmonate ( MeJA ) was studied by fluorescence and UV-vis spectroscopic techniques .
[ Sen. 5, subscore: 1.00 ]: The binding sites of MeJA with the coiled-coil structural domain of rice blast resistance protein Pi36 were found to approach the microenvironment of both Tyr and Trp by the synchronous fluorescence spectrometry .
[ Sen. 6, subscore: 1.00 ]: The distance r between donor ( the coiled-coil domain of rice blast resistance protein Pi36 ) and acceptor ( MeJA ) was obtained according to Forster theory of non-radioactive energy transfer .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1 .
| | Author: Lin F Chen S Que Z Wang L Liu X Pan Q | | Journal: Genetics Citation: V : 177 P : 1871-80 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17947408 Accession (PMID): 17947408 | | Abstract: The resistance ( R ) gene Pi37 , present in the rice cultivar St No 1 , was isolated by an in silico map-based cloning procedure .
The equivalent genetic region in Nipponbare contains four nucleotide binding site-leucine-rich repeat ( NBS-LRR ) type loci .
These four candidates for Pi37 ( Pi37-1 , -2 , -3 , and -4 ) were amplified separately from St No 1 via long-range PCR , and cloned into a binary vector .
Each construct was individually transformed into the highly blast susceptible cultivar Q1063 .
The subsequent complementation analysis revealed Pi37-3 to be the functional gene , while -1 , -2 , and -4 are probably pseudogenes .
Pi37 encodes a 1290 peptide NBS-LRR product , and the presence of substitutions at two sites in the NBS region ( V239A and I247M ) is associated with the resistance phenotype .
Semiquantitative expression analysis showed that in St No 1 , Pi37 was constitutively expressed and only slightly induced by blast infection .
Transient expression experiments indicated that the Pi37 product is restricted to the cytoplasm .
Pi37-3 is thought to have evolved recently from -2 , which in turn was derived from an ancestral -1 sequence .
Pi37-4 is likely the most recently evolved member of the cluster and probably represents a duplication of -3 .
The four Pi37 paralogs are more closely related to maize rp1 than to any of the currently isolated rice blast R genes Pita , Pib , Pi9 , Pi2 , Piz-t , and Pi36 .
| Matching Sentences:
[ Sen. 11, subscore: 1.00 ]: The four Pi37 paralogs are more closely related to maize rp1 than to any of the currently isolated rice blast R genes Pita , Pib , Pi9 , Pi2 , Piz-t , and Pi36 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Genetic and physical mapping of Pi36 ( t ) , a novel rice blast resistance gene located on rice chromosome 8 .
| | Author: Liu XQ Wang L Chen S Lin F Pan QH .
| | Journal: Mol . Genet . Genomics Citation: V : 274 ( 4 ) P : 394-401 Year: 2005 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub16151856 Accession (PMID): 16151856 | | Abstract: Blast resistance in the indica cultivar ( cv . ) Q61 was inherited as a single dominant gene in two F2 populations , F2-1 and F2-2 , derived from crosses between the donor cv . and two susceptible japonica cvs Aichi Asahi and Lijiangxintuanheigu ( LTH ) , respectively .
To rapidly determine the chromosomal location of the resistance ( R ) gene detected in Q61 , random amplified polymorphic DNA ( RAPD ) analysis was performed in the F2-1 population using bulked-segregant analysis ( BSA ) in combination with recessive-class analysis ( RCA ) .
One of the three linked markers identified , BA1126 ( 550 ) , was cloned and sequenced .
The R gene locus was roughly mapped on rice chromosome 8 by comparison of the BA1126 ( 550 ) sequence with rice sequences in the databases ( chromosome landing ) .
To confirm this finding , seven known markers , including four sequence-tagged-site ( STS ) markers and three simple-sequence repeat ( SSR ) markers flanking BA1126 ( 550 ) on chromosome 8 , were subjected to linkage analysis in the two F2 populations .
The locus was mapped to a 5 . 8 cM interval bounded by RM5647 and RM8018 on the short arm of chromosome 8 .
This novel R gene is therefore tentatively designated as Pi36 ( t ) .
For fine mapping of the Pi36 ( t ) locus , five additional markers including one STS marker and four candidate resistance gene ( CRG ) markers were developed in the target region , based on the genomic sequence of the corresponding region of the reference japonica cv .
Nipponbare .
The Pi36 ( t ) locus was finally localized to an interval of about 0 . 6 cM flanked by the markers RM5647 and CRG2 , and co-segregated with the markers CRG3 and CRG4 .
To physically map this locus , the Pi36 ( t ) -linked markers were mapped by electronic hybridization to bacterial artificial chromosome ( BAC ) or P1 artificial chromosome ( PAC ) clones of Nipponbare , and a contig map was constructed in silico through Pairwise BLAST analysis .
The Pi36 ( t ) locus was physically delimited to an interval of about 17 . 0 kb , based on the genomic sequence of Nipponbare . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Genetic and physical mapping of Pi36 ( t ) , a novel rice blast resistance gene located on rice chromosome 8 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
