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| Title: A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae .
| | Author: Chen J Shi Y Liu W Chai R Fu Y Zhuang J Wu J | | Journal: J Genet Genomics Citation: V : 38 P : 209-16 Year: 2011 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub21621742 Accession (PMID): 21621742 | | Abstract: Rice blast , caused by Magnaporthe oryzae , is one of the most devastating diseases .
Using map-based strategy and in silico approach we isolated a new rice ( Oryza sativa L ) blast resistance allele of Pid3 , designated Pi25 , from a stable blast resistance cultivar Gumei2 .
Over-expression analysis and complementation test showed that Pi25 conferred blast resistance to M oryzae isolate js001-20 .
Sequence analysis showed that Pi25 was an intronless gene of 2 , 772 nucleotides with single nucleotide substitution in comparison to Pid3 at the nucleotide position 459 and predicatively encoded a typical coiled coil--nucleotide binding site--leucine rich repeat ( CC--NBS--LRR ) protein of 924 amino acid residuals with 100% identity to Pid3 putative protein .
The susceptible allele pi25 in Nipponbare contained a nonsense mutation at the nucleotide position 2 , 209 resulting in a truncated protein with 736 amino acid residuals .
In addition , 14 nucleotide substitutions resulting in 10 amino acid substitutions were identified between Pi25 and pi25 upstream the premature stop codon in the susceptible allele .
Although the mechanism of Pi25/Pid3-mediated resistance needs to be further investigated , the isolation of the allele would facilitate the utilization of Pi25/Pid3 in rice blast resistance breeding program via transgenic approach and marker assisted selection .
| Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: In addition , 14 nucleotide substitutions resulting in 10 amino acid substitutions were identified between Pi25 and pi25 upstream the premature stop codon in the susceptible allele .
[ Sen. 2, subscore: 1.00 ]: Using map-based strategy and in silico approach we isolated a new rice ( Oryza sativa L ) blast resistance allele of Pid3 , designated Pi25 , from a stable blast resistance cultivar Gumei2 .
[ Sen. 3, subscore: 1.00 ]: Over-expression analysis and complementation test showed that Pi25 conferred blast resistance to M oryzae isolate js001-20 .
[ Sen. 4, subscore: 1.00 ]: Sequence analysis showed that Pi25 was an intronless gene of 2 , 772 nucleotides with single nucleotide substitution in comparison to Pid3 at the nucleotide position 459 and predicatively encoded a typical coiled coil--nucleotide binding site--leucine rich repeat ( CC--NBS--LRR ) protein of 924 amino acid residuals with 100% identity to Pid3 putative protein .
[ Sen. 5, subscore: 1.00 ]: The susceptible allele pi25 in Nipponbare contained a nonsense mutation at the nucleotide position 2 , 209 resulting in a truncated protein with 736 amino acid residuals .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Genetic control of rice blast resistance in the durably resistant cultivar Gumei 2 against multiple isolates .
| | Author: Wu JL Fan YY Li DB Zheng KL Leung H Zhuang JY .
| | Journal: Theor . Appl . Genet . Citation: V : 111 ( 1 ) P : 50-6 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15856160 Accession (PMID): 15856160 | | Abstract: To further our understanding of the genetic control of blast resistance in rice cultivar Gumei 2 and , consequently , to facilitate the utilization of this durably blast-resistant cultivar , we studied 304 recombinant inbred lines of indica rice cross Zhong 156/Gumei 2 and a linkage map comprising 181 markers .
An analysis of segregation for resistance against five isolates of rice blast suggested that one gene cluster and three additional major genes that are independently inherited are responsible for the complete resistance of Gumei 2 .
The gene cluster was located to chromosome 6 and includes two genes mapped previously , Pi25 ( t ) , against Chinese rice blast isolate 92-183 ( race ZC15 ) and Pi26 ( t ) against Philippine rice blast isolate Ca89 ( lineage 4 ) , and a gene for resistance against Philippine rice blast isolate 92330-5 ( lineage 17 ) .
Of the two genes conferring resistance against the Philippine isolates V86013 ( lineage 15 ) and C923-39 ( lineage 46 ) , we identified one as Pi26 ( t ) and mapped the other onto the distal end of chromosome 2 where Pib is located .
We used three components of partial blast resistance , percentage diseased leaf area ( DLA ) , lesion number and lesion size , all measured in the greenhouse , to measure the degree of susceptibility to isolates Ca89 and C923-39 and subsequently identified nine and eight quantitative trait loci ( QTLs ) , respectively .
Epistasis was determined to play an important role in partial resistance against Ca89 .
Using DLA measured on lines susceptible in a blast nursery , we detected six QTLs .
While different QTLs were detected for partial resistance to Ca89 and C923-39 , respectively , most were involved in the partial resistance in the field .
Our results suggest that the blast resistance in Gumei 2 is controlled by multiple major genes and minor genes with epistatic effects . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The gene cluster was located to chromosome 6 and includes two genes mapped previously , Pi25 ( t ) , against Chinese rice blast isolate 92-183 ( race ZC15 ) and Pi26 ( t ) against Philippine rice blast isolate Ca89 ( lineage 4 ) , and a gene for resistance against Philippine rice blast isolate 92330-5 ( lineage 17 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
