| 69 matches found in 33 documents. Search time: 0.156 seconds. |
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Score: 15.00 |
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| Title: Isolation and characterization of PHYC gene from Stellaria longipes : differential expression regulated by different red/far-red light ratios and photoperiods .
| | Author: Li WZ Chinnappa CC .
| | Journal: Planta Citation: V : 220 ( 2 ) P : 318-30 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15290294 Accession (PMID): 15290294 | | Abstract: We have cloned and characterized the phytochrome C ( PHYC ) gene from Stellaria longipes .
The PHYC gene is composed of a 110-bp 5-untranslated leader sequence , a 3 , 342-bp coding region , and a 351-bp 3-untranslated sequence .
The Stellaria PHYC contains three long introns within the coding region at conserved locations as in most angiosperm PHY genes .
DNA blot analysis indicates that the Stellaria genome contains a single copy of PHYC .
Stellaria PHYC shares 60% , 58% , and 57% deduced amino acid identities with rice , Sorghum , and Arabidopsis PHYC , respectively .
Phylogenetic analysis indicates that Stellaria PHYC is located in the dicot branch , but is divergent from Arabidopsis PHYC .
The Stellaria PHYC is constitutively expressed in different plant organs , though the level of PHYC gene transcript in roots is slightly higher than in flowers , leaves , and stems .
When 2-week old seedlings grown in the dark were exposed to constant white light , PHYC mRNA quickly accumulates within 1-12 h .
When plants grown in darkness for 7 days were exposed to different red/far-red light ( R/FR ) ratios , the levels of PHYC mRNA at R/FR = 0 . 7 are much lower than under R/FR = 3 . 5 .
The levels of PHYC mRNA under short-day ( SD ) photoperiod are higher than under long-day ( LD ) photoperiod .
Plants under SD conditions do not elongate , and are only about 1 . 7 cm tall at 19 days .
In contrast , plants under LD conditions elongate with an average height of 21 . 2 cm at 19 days .
The plants do not flower under SD conditions , but do so at 18-19 days under LD conditions .
These results indicate that under SD conditions the high level of PHYC mRNA may inhibit stem elongation and flower initiation .
In contrast , under LD conditions the high level of PHYC mRNA may promote stem elongation and flowering .
| Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Stellaria PHYC shares 60% , 58% , and 57% deduced amino acid identities with rice , Sorghum , and Arabidopsis PHYC , respectively .
[ Sen. 6, subscore: 2.00 ]: Phylogenetic analysis indicates that Stellaria PHYC is located in the dicot branch , but is divergent from Arabidopsis PHYC .
[ Sen. 7, subscore: 2.00 ]: The Stellaria PHYC is constitutively expressed in different plant organs , though the level of PHYC gene transcript in roots is slightly higher than in flowers , leaves , and stems .
[ Sen. 1, subscore: 1.00 ]: We have cloned and characterized the phytochrome C ( PHYC ) gene from Stellaria longipes .
[ Sen. 2, subscore: 1.00 ]: The PHYC gene is composed of a 110-bp 5-untranslated leader sequence , a 3 , 342-bp coding region , and a 351-bp 3-untranslated sequence .
[ Sen. 3, subscore: 1.00 ]: The Stellaria PHYC contains three long introns within the coding region at conserved locations as in most angiosperm PHY genes .
[ Sen. 4, subscore: 1.00 ]: DNA blot analysis indicates that the Stellaria genome contains a single copy of PHYC .
[ Sen. 8, subscore: 1.00 ]: When 2-week old seedlings grown in the dark were exposed to constant white light , PHYC mRNA quickly accumulates within 1-12 h .
[ Sen. 9, subscore: 1.00 ]: When plants grown in darkness for 7 days were exposed to different red/far-red light ( R/FR ) ratios , the levels of PHYC mRNA at R/FR = 0 . 7 are much lower than under R/FR = 3 . 5 .
[ Sen. 10, subscore: 1.00 ]: The levels of PHYC mRNA under short-day ( SD ) photoperiod are higher than under long-day ( LD ) photoperiod .
[ Sen. 14, subscore: 1.00 ]: These results indicate that under SD conditions the high level of PHYC mRNA may inhibit stem elongation and flower initiation .
[ Sen. 15, subscore: 1.00 ]: In contrast , under LD conditions the high level of PHYC mRNA may promote stem elongation and flowering .
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Score: 11.00 |
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| Title: Rice PHYC gene : structure , expression , map position and evolution .
| | Author: Basu D Dehesh K Schneider-Poetsch HJ Harrington SE McCouch SR Quail PH .
| | Journal: Plant Mol . Biol . Citation: V : 44 ( 1 ) P : 27-42 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11094977 Accession (PMID): 11094977 | | Abstract: Although sequences representing members of the phytochrome ( phy ) family of photoreceptors have been reported in numerous species across the phylogenetic spectrum , relatively few phytochrome genes ( PHY ) have been fully characterized .
Using rice , we have cloned and characterized the first PHYC gene from a monocot .
Comparison of genomic and cDNA PHYC sequences shows that the rice PHYC gene contains three introns in the protein-coding region typical of most angiosperm PHY genes , in contrast to Arabidopsis PHYC , which lacks the third intron .
Mapping of the transcription start site and 5-untranslated region of the rice PHYC transcript indicates that it contains an unusually long , intronless , 5-untranslated leader sequence of 715 bp .
PHYC mRNA levels are relatively low compared to PHYA and PHYB mRNAs in rice seedlings , and are similar in dark and light-treated seedlings , suggesting relatively low constitutive expression .
Genomic mapping shows that the PHYA , PHYB , and PHYC genes are all located on chromosome 3 of rice , in synteny with these genes in linkage group C ( sometimes referred to as linkage group A ) of sorghum .
Phylogenetic analysis indicates that rice phyC is closely related to sorghum phyC , but relatively strongly divergent from Arabidopsis phyC , the only full-length dicot phyC sequence available . | Matching Sentences:
[ Sen. 7, subscore: 4.00 ]: Phylogenetic analysis indicates that rice phyC is closely related to sorghum phyC , but relatively strongly divergent from Arabidopsis phyC , the only full-length dicot phyC sequence available .
[ Sen. 3, subscore: 3.00 ]: Comparison of genomic and cDNA PHYC sequences shows that the rice PHYC gene contains three introns in the protein-coding region typical of most angiosperm PHY genes , in contrast to Arabidopsis PHYC , which lacks the third intron .
[ Sen. 2, subscore: 1.00 ]: Using rice , we have cloned and characterized the first PHYC gene from a monocot .
[ Sen. 4, subscore: 1.00 ]: Mapping of the transcription start site and 5-untranslated region of the rice PHYC transcript indicates that it contains an unusually long , intronless , 5-untranslated leader sequence of 715 bp .
[ Sen. 5, subscore: 1.00 ]: PHYC mRNA levels are relatively low compared to PHYA and PHYB mRNAs in rice seedlings , and are similar in dark and light-treated seedlings , suggesting relatively low constitutive expression .
[ Sen. 6, subscore: 1.00 ]: Genomic mapping shows that the PHYA , PHYB , and PHYC genes are all located on chromosome 3 of rice , in synteny with these genes in linkage group C ( sometimes referred to as linkage group A ) of sorghum .
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Score: 7.00 |
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| Title: Structural characterization , expression analysis and evolution of the red/far-red sensing photoreceptor gene , phytochrome C ( PHYC ) , localized on the B genome of hexaploid wheat ( Triticum aestivum L ) .
| | Author: Kulshreshtha R Kumar N Balyan HS Gupta PK Khurana P Tyagi AK Khurana JP .
| | Journal: Planta Citation: V : 221 ( 5 ) P : 675-89 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15891901 Accession (PMID): 15891901 | | Abstract: Phytochromes are a family of red/far-red light perceiving photoreceptors . The monocot phytochrome family is represented by three members , PHYA , PHYB and PHYC .
We have isolated and characterized the first PHY gene member ( TaPHYC ) from common wheat , Triticum aestivum var .
CPAN1676 .
It codes for a species of the photoreceptor , phyC , which is known to be light-stable in all plants analyzed so far .
A sequence of 7 . 2 kb has been determined , which includes 3 . 42 kb of coding region . This is the second full-length PHYC gene sequenced from a monocot ( first was from rice ) .
TaPHYC gene shares structural similarities with the rice PHYC containing four exons and three introns in the coding region .
The 5 UTR is 1 . 0-kb-long and harbors an upstream open reading frame ( URF ) encoding 28 aa .
Southern blot analysis of TaPHYC indicates that it represents single locus in the wheat genome , although the possibility of additional loci can not be completely ruled out .
Chromosomal localization using nullisomic-tetrasomic lines of Triticum aestivum var .
Chinese Spring places TaPHYC on chromosome 4B .
PHYC represents a constitutively expressed gene in all the organs tested and under light/dark conditions .
However , PHYC was found to be developmentally regulated showing maximal expression in 3-day-old dark-grown seedlings , which declined thereafter .
In silico analysis has also been done to compare TaPHYC gene with the partial sequences known from other wheat species and cultivars .
The presence of a topoisomerase gene immediately downstream of the PHYC gene , both in rice and wheat genomes , presents yet another example of synteny in cereals and its possible significance has been discussed .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Phytochromes are a family of red/far-red light perceiving photoreceptors . The monocot phytochrome family is represented by three members , PHYA , PHYB and PHYC .
[ Sen. 4, subscore: 1.00 ]: It codes for a species of the photoreceptor , phyC , which is known to be light-stable in all plants analyzed so far .
[ Sen. 5, subscore: 1.00 ]: A sequence of 7 . 2 kb has been determined , which includes 3 . 42 kb of coding region . This is the second full-length PHYC gene sequenced from a monocot ( first was from rice ) .
[ Sen. 6, subscore: 1.00 ]: TaPHYC gene shares structural similarities with the rice PHYC containing four exons and three introns in the coding region .
[ Sen. 11, subscore: 1.00 ]: PHYC represents a constitutively expressed gene in all the organs tested and under light/dark conditions .
[ Sen. 12, subscore: 1.00 ]: However , PHYC was found to be developmentally regulated showing maximal expression in 3-day-old dark-grown seedlings , which declined thereafter .
[ Sen. 14, subscore: 1.00 ]: The presence of a topoisomerase gene immediately downstream of the PHYC gene , both in rice and wheat genomes , presents yet another example of synteny in cereals and its possible significance has been discussed .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 7.00 |
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| Title: Distinct and cooperative functions of phytochromes A , B , and C in the control of deetiolation and flowering in rice .
| | Author: Takano M Inagaki N Xie X Yuzurihara N Hihara F Ishizuka T Yano M Nishimura M Miyao A Hirochika H Shinomura T | | Journal: Plant Cell Citation: V : 17 ( 12 ) P : 3311-25 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16278346 Accession (PMID): 16278346 | | Abstract: We have isolated phytochrome B ( phyB ) and phyC mutants from rice ( Oryza sativa ) and have produced all combinations of double mutants .
Seedlings of phyB and phyB phyC mutants exhibited a partial loss of sensitivity to continuous red light ( Rc ) but still showed significant deetiolation responses .
The responses to Rc were completely canceled in phyA phyB double mutants .
These results indicate that phyA and phyB act in a highly redundant manner to control deetiolation under Rc .
Under continuous far-red light ( FRc ) , phyA mutants showed partially impaired deetiolation , and phyA phyC double mutants showed no significant residual phytochrome responses , indicating that not only phyA but also phyC is involved in the photoperception of FRc in rice .
Interestingly , the phyB phyC double mutant displayed clear R/FR reversibility in the pulse irradiation experiments , indicating that both phyA and phyB can mediate the low-fluence response for gene expression .
Rice is a short-day plant , and we found that mutation in either phyB or phyC caused moderate early flowering under the long-day photoperiod , while monogenic phyA mutation had little effect on the flowering time .
The phyA mutation , however , in combination with phyB or phyC mutation caused dramatic early flowering . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Under continuous far-red light ( FRc ) , phyA mutants showed partially impaired deetiolation , and phyA phyC double mutants showed no significant residual phytochrome responses , indicating that not only phyA but also phyC is involved in the photoperception of FRc in rice .
[ Sen. 1, subscore: 1.00 ]: We have isolated phytochrome B ( phyB ) and phyC mutants from rice ( Oryza sativa ) and have produced all combinations of double mutants .
[ Sen. 2, subscore: 1.00 ]: Seedlings of phyB and phyB phyC mutants exhibited a partial loss of sensitivity to continuous red light ( Rc ) but still showed significant deetiolation responses .
[ Sen. 6, subscore: 1.00 ]: Interestingly , the phyB phyC double mutant displayed clear R/FR reversibility in the pulse irradiation experiments , indicating that both phyA and phyB can mediate the low-fluence response for gene expression .
[ Sen. 7, subscore: 1.00 ]: Rice is a short-day plant , and we found that mutation in either phyB or phyC caused moderate early flowering under the long-day photoperiod , while monogenic phyA mutation had little effect on the flowering time .
[ Sen. 8, subscore: 1.00 ]: The phyA mutation , however , in combination with phyB or phyC mutation caused dramatic early flowering .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: Comprehensive metabolite profiling of phyA phyB phyC triple mutants to reveal their associated metabolic phenotype in rice leaves .
| | Author: Jumtee K Okazawa A Harada K Fukusaki E Takano M Kobayashi A | | Journal: J Biosci Bioeng Citation: V : 108 P : 151-9 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19619864 Accession (PMID): 19619864 | | Abstract: The phytochrome photoreceptors regulate plant growth and development throughout their life cycle .
Rice ( Oryza sativa ) possesses three phytochromes , phyA , phyB , and phyC .
Physiological , genetic , and biochemical analyses of null mutants of each phytochrome have revealed the function of each in rice .
However , few studies have investigated the relationship between phytochrome signaling and metabolism .
In the present study , non-targeted metabolite analysis by gas chromatography time-of-flight mass spectrometry ( GC/TOF-MS ) and targeted metabolite analysis by capillary electrophoresis electrospray ionization mass spectrometry ( CE/ESI-MS ) were employed to investigate metabolic changes in rice phyA phyB phyC triple mutants .
Distinct metabolic profiles between phyA phyB phyC triple mutants and the wild type ( WT ) , as well as those between young and mature leaf blades , could be clearly observed by principal component analysis ( PCA ) .
The metabolite profiles indicated high accumulation of amino acids , organic acids , sugars , sugar phosphates , and nucleotides in the leaf blades of phyA phyB phyC triple mutants , especially in the young leaves , compared with those in the WT .
Remarkable overaccumulation of monosaccharide , such as glucose ( 53 . 4-fold ) , fructose ( 42 . 5-fold ) , and galactose ( 24 . 5-fold ) , was observed in young leaves of phyA phyB phyC triple mutants .
These metabolic phenotypes suggest that sugar metabolism , carbon partitioning , sugar transport , or some combination of these is impaired in the phyA phyB phyC triple mutants , and conversely , that phytochromes have crucial roles in sugar metabolism .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Rice ( Oryza sativa ) possesses three phytochromes , phyA , phyB , and phyC .
[ Sen. 5, subscore: 1.00 ]: In the present study , non-targeted metabolite analysis by gas chromatography time-of-flight mass spectrometry ( GC/TOF-MS ) and targeted metabolite analysis by capillary electrophoresis electrospray ionization mass spectrometry ( CE/ESI-MS ) were employed to investigate metabolic changes in rice phyA phyB phyC triple mutants .
[ Sen. 6, subscore: 1.00 ]: Distinct metabolic profiles between phyA phyB phyC triple mutants and the wild type ( WT ) , as well as those between young and mature leaf blades , could be clearly observed by principal component analysis ( PCA ) .
[ Sen. 7, subscore: 1.00 ]: The metabolite profiles indicated high accumulation of amino acids , organic acids , sugars , sugar phosphates , and nucleotides in the leaf blades of phyA phyB phyC triple mutants , especially in the young leaves , compared with those in the WT .
[ Sen. 8, subscore: 1.00 ]: Remarkable overaccumulation of monosaccharide , such as glucose ( 53 . 4-fold ) , fructose ( 42 . 5-fold ) , and galactose ( 24 . 5-fold ) , was observed in young leaves of phyA phyB phyC triple mutants .
[ Sen. 9, subscore: 1.00 ]: These metabolic phenotypes suggest that sugar metabolism , carbon partitioning , sugar transport , or some combination of these is impaired in the phyA phyB phyC triple mutants , and conversely , that phytochromes have crucial roles in sugar metabolism .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
