| 12 matches found in 4 documents. Search time: 0.115 seconds. |
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Score: 5.00 |
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| Title: Functional properties of a phenylalanine ammonia-lyase promoter from Arabidopsis .
| | Author: Ohl S Hedrick SA Chory J Lamb CJ .
| | Journal: Plant Cell Citation: V : 2 ( 9 ) P : 837-48 Year: 1990 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub2152131 Accession (PMID): 2152131 | | Abstract: Phenylalanine ammonia-lyase ( PAL ) is encoded by a small family of genes in Arabidopsis .
We cloned and partially characterized one of these genes , PAL1 .
The deduced amino acid sequence is highly similar to PAL from bean , parsley , and rice .
The promoter contains sequence elements homologous to two putative regulatory elements conserved among several phenylpropanoid genes .
The regulation of the PAL1 gene was examined by analysis of beta-glucuronidase ( GUS ) activity in transgenic Arabidopsis containing PAL1-GUS gene fusions .
The PAL1 promoter was activated early in seedling development and in adult plants was strongly expressed in the vascular it issues of roots and leaves , but was not active in the root tip or the shoot apical meristem .
In flowers , expression was observed in sepals , anthers , and carpels , but not in petals .
Transcripts encoded by the endogenous PAL genes and GUS transcripts from the PAL1-GUS gene fusion were induced by wounding , HgCl2-stress , and light .
Analysis of the regulatory properties of 5 deleted promoters showed that the proximal region of the promoter to -290 was sufficient to establish the full it issue-specific pattern of expression and that the proximal region to -540 was responsive to environmental stimuli .
Negative and positive elements were located between -1816 and -823 and between -823 and -290 , respectively .
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[ Sen. 5, subscore: 2.00 ]: The regulation of the PAL1 gene was examined by analysis of beta-glucuronidase ( GUS ) activity in transgenic Arabidopsis containing PAL1-GUS gene fusions .
[ Sen. 2, subscore: 1.00 ]: We cloned and partially characterized one of these genes , PAL1 .
[ Sen. 6, subscore: 1.00 ]: The PAL1 promoter was activated early in seedling development and in adult plants was strongly expressed in the vascular it issues of roots and leaves , but was not active in the root tip or the shoot apical meristem .
[ Sen. 8, subscore: 1.00 ]: Transcripts encoded by the endogenous PAL genes and GUS transcripts from the PAL1-GUS gene fusion were induced by wounding , HgCl2-stress , and light .
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Score: 4.00 |
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| Title: The bHLH Rac Immunity1 ( RAI1 ) Is Activated by OsRac1 via OsMAPK3 and OsMAPK6 in Rice Immunity .
| | Author: Kim SH Oikawa T Kyozuka J Wong HL Umemura K Kishi-Kaboshi M Takahashi A Kawano Y Kawasaki T Shimamoto K | | Journal: Plant Cell Physiol Citation: V : 53 P : 740-54 Year: 2012 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22437844 Accession (PMID): 22437844 | | Abstract: The Rac/Rop GTPase OsRac1 plays an essential role in rice immunity .
However , the regulatory genes acting downstream of OsRac1 are largely unknown .
We focused on the RAI1 gene , which is up-regulated in suspension cells expressing a constitutively active form of OsRac1 .
RAI1 encodes a putative basic helix-loop-helix transcription factor .
A microarray analysis of cells transformed with an inducible RAI1 construct showed increased expression of PAL1 and OsWRKY19 genes after induction , suggesting that these genes are regulated by RAI1 .
This was confirmed using RAI1 T-DNA activation-tagged and RNA interference lines .
The PAL1 and OsWRKY19 genes were also up-regulated by sphingolipid and chitin elicitors , and the RAI1 activation-tagged plants had increased resistance to a rice blast fungus .
These results indicated that RAI1 is involved in defense responses in rice .
RAI1 interacted with OsMAPK3 and OsMAPK6 proteins in vivo and in vitro .
Also , RAI1 was phosphorylated by OsMAPK3/6 and OsMKK4-dd in vitro .
Overexpression of OsMAPK6 and/or OsMAPK3 together with OsMKK4-dd increased PAL1 and OsWRKY19 expression in rice protoplasts .
Therefore , the regulation of PAL1 and OsWRKY19 expression by RAI1 could be controlled via an OsMKK4-OsMAPK3/6 cascade .
Co-immunoprecipitation assays indicated that OsMAPK3 and OsRac1 occur in the same complex as OsMAPK6 .
Taken together , our results indicate that RAI1 could be regulated by OsRac1 through an OsMAPK3/6 cascade .
In this study , we have identified RAI1 as the first transcription factor acting downstream of OsRac1 .
This work will help us to understand the immune system regulated by OsRac1 in rice and its orthologs in other plant species .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: A microarray analysis of cells transformed with an inducible RAI1 construct showed increased expression of PAL1 and OsWRKY19 genes after induction , suggesting that these genes are regulated by RAI1 .
[ Sen. 7, subscore: 1.00 ]: The PAL1 and OsWRKY19 genes were also up-regulated by sphingolipid and chitin elicitors , and the RAI1 activation-tagged plants had increased resistance to a rice blast fungus .
[ Sen. 11, subscore: 1.00 ]: Overexpression of OsMAPK6 and/or OsMAPK3 together with OsMKK4-dd increased PAL1 and OsWRKY19 expression in rice protoplasts .
[ Sen. 12, subscore: 1.00 ]: Therefore , the regulation of PAL1 and OsWRKY19 expression by RAI1 could be controlled via an OsMKK4-OsMAPK3/6 cascade .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Identification and fine mapping of a mutant gene for palealess spikelet in rice .
| | Author: Luo Q Zhou K Zhao X Zeng Q Xia H Zhai W Xu J Wu X Yang H Zhu L | | Journal: Planta Citation: V : 221 ( 2 ) P : 222-30 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15605239 Accession (PMID): 15605239 | | Abstract: In grass , the evolutionary relationship between lemma and palea , and their relationship to the flower organs in dicots have been variously interpreted and wildely debated .
In the present study , we carried out morphological and genetic analysis of a palealess mutant ( pal ) from rice ( Oryza sativa L ) , and fine mapping the gene responsible for the mutated trait .
Together , our findings indicate that the palea is replaced by two leaf-like structures in the pal flowers , and this trait is controlled by one recessive gene , termed palealess1 ( pal1 ) .
With a large F2 segregating population , the pal1 gene was finally mapped into a physical region of 35 kb .
Our results also suggest that the lemma and palea of rice are not homologous organs , palea is likely evolutionarily equivalent to the eudicot sepal , and the pal1 should be an A function gene for rice floral organ identity . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: Together , our findings indicate that the palea is replaced by two leaf-like structures in the pal flowers , and this trait is controlled by one recessive gene , termed palealess1 ( pal1 ) .
[ Sen. 4, subscore: 1.00 ]: With a large F2 segregating population , the pal1 gene was finally mapped into a physical region of 35 kb .
[ Sen. 5, subscore: 1.00 ]: Our results also suggest that the lemma and palea of rice are not homologous organs , palea is likely evolutionarily equivalent to the eudicot sepal , and the pal1 should be an A function gene for rice floral organ identity .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Molecular cloning and expression analysis of ultraspiracle ( USP ) from the rice stem borer Chilo suppressalis .
| | Author: Minakuchi C Nakagawa Y Kiuchi M Seino A Tomita S Kamimura M | | Journal: Insect Biochem . Mol . Biol . Citation: V : 33 ( 1 ) P : 41-9 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12459199 Accession (PMID): 12459199 | | Abstract: cDNA for ultraspiracle ( USP ) from the lepidopteran rice stem borer Chilo suppressalis was cloned using PCR techniques .
The deduced amino acid sequence of C suppressalis USP ( CsUSP ) was very similar to those of other lepidopteran USPs , especially to the Manduca sexta USP-2 isoform .
Northern hybridization analysis detected a 6 . 5-kb message in the epidermis , fat body , and midgut of wandering larvae .
CsUSP mRNA expression in the epidermis varied little during the last larval instar .
Gel mobility shift assays showed that in vitro translated C suppressalis ecdysone receptor ( CsEcR ) and CsUSP proteins bound to the Pal1 or Drosophila melanogaster hsp27 ecdysone response element as a heterodimer .
In a ligand-receptor binding assay , [ ( 3 ) H ] ponasterone A ( [ ( 3 ) H ] PoA ) did not bind to individual CsEcR or CsUSP protein , but bound strongly to the CsEcR/CsUSP complex [ ( 3 ) H ] PoA binding to CsEcR/CsUSP complex was competed by 20-hydroxyecdysone and a non-steroidal ecdysteroid agonist , RH-5992 , but not by cholesterol , indicating that compounds with molting hormone activity against C suppressalis can bind specifically to the CsEcR/CsUSP complex | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: Gel mobility shift assays showed that in vitro translated C suppressalis ecdysone receptor ( CsEcR ) and CsUSP proteins bound to the Pal1 or Drosophila melanogaster hsp27 ecdysone response element as a heterodimer .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
