| 12 matches found in 5 documents. Search time: 0.587 seconds. |
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Score: 4.00 |
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| Title: Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis .
| | Author: Postma-Haarsma AD Verwoert II Stronk OP Koster J Lamers GE Hoge JH Meijer AH .
| | Journal: Plant Mol . Biol . Citation: V : 39 ( 2 ) P : 257-71 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10080693 Accession (PMID): 10080693 | | Abstract: For identification of genes involved in embryogenesis in the model cereal rice , we have constructed a collection of cDNA libraries of well-defined stages of embryo development before , during and after organ differentiation .
Here , we focus on the possible role of KNOX ( maize Knotted1-like ) class homeobox genes in regulation of rice embryogenesis .
Three types of KNOX clones were identified in libraries of early zygotic embryos .
Two of these , Oskn2 and Oskn3 , encode newly described KNOX genes , whereas the third ( Oskn1 ) corresponds to the previously described OSH1 gene .
In situ hybridizations showed that during the early stages of embryo development , all three KNOX genes are expressed in the region where the shoot apical meristem ( SAM ) is organizing , suggesting that these genes are involved in regulating SAM formation .
Whereas OSH1 was previously proposed to function also in SAM maintenance , Oskn3 may be involved in patterning organ positions , as its expression was found to mark the boundaries of different embryonic organs following SAM formation .
The expression pattern of Oskn2 suggested an additional role in scutellum and epiblast development .
Transgenic expression of Oskn2 and Oskn3 in tobacco further supported their involvement in cell fate determination , like previously reported for Knotted1 and OSH1 ectopic expression .
Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development , Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology .
The observation that the KNOX genes produce similar though distinct phenotypic reponses in tobacco , indicates that their gene products act on overlapping but different sets of target genes , or that cell-type specific factors determine their precise action . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Two of these , Oskn2 and Oskn3 , encode newly described KNOX genes , whereas the third ( Oskn1 ) corresponds to the previously described OSH1 gene .
[ Sen. 7, subscore: 1.00 ]: The expression pattern of Oskn2 suggested an additional role in scutellum and epiblast development .
[ Sen. 8, subscore: 1.00 ]: Transgenic expression of Oskn2 and Oskn3 in tobacco further supported their involvement in cell fate determination , like previously reported for Knotted1 and OSH1 ectopic expression .
[ Sen. 9, subscore: 1.00 ]: Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development , Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
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| Title: Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice .
| | Author: Postma-Haarsma AD Rueb S Scarpella E den Besten W Hoge JH Meijer AH .
| | Journal: Plant Mol . Biol . Citation: V : 48 ( 4 ) P : 423-41 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub11908517 Accession (PMID): 11908517 | | Abstract: Plant homeobox genes of the class 1 knox ( knotted1-like ) type are involved in the regulation of shoot apical meristem formation and function .
Their expression generally occurs either throughout the meristem or specifically at the lateral organ boundaries .
Down-regulation in the organ primordia is tightly controlled and misexpression in leaves leads to a perturbed development .
Here , the transcriptional control of two rice knox genes , Oskn2 and Oskn3 , was addressed , showing that the promoter sequences of both genes mediate the initial down-regulation during lateral organ formation , but are insufficient to keep expression in lateral organs stably off .
Therefore , maintenance of the repressed state requires control elements outside the promoter regions .
Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching . internode elongation and leaf patterning .
However , small differences suggested that their target gene specificities are not completely overlapping .
This was supported by the observation that Oskn3 protein but not Oskn2 could interact with two reported recognition sequences of a KNOX protein from barley .
Finally , protein-protein interactions may contribute to the functioning of KNOX proteins , as the ability of Oskn3 and Oskn2 to form heterodimers could be demonstrated . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: Here , the transcriptional control of two rice knox genes , Oskn2 and Oskn3 , was addressed , showing that the promoter sequences of both genes mediate the initial down-regulation during lateral organ formation , but are insufficient to keep expression in lateral organs stably off .
[ Sen. 6, subscore: 1.00 ]: Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching . internode elongation and leaf patterning .
[ Sen. 8, subscore: 1.00 ]: This was supported by the observation that Oskn3 protein but not Oskn2 could interact with two reported recognition sequences of a KNOX protein from barley .
[ Sen. 9, subscore: 1.00 ]: Finally , protein-protein interactions may contribute to the functioning of KNOX proteins , as the ability of Oskn3 and Oskn2 to form heterodimers could be demonstrated .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Different subcellular localization and trafficking properties of KNOX class 1 homeodomain proteins from rice .
| | Author: Kuijt SJ Lamers GE Rueb S Scarpella E Ouwerkerk PB Spaink HP Meijer AH .
| | Journal: Plant Mol . Biol . Citation: V : 55 ( 6 ) P : 781-96 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15604716 Accession (PMID): 15604716 | | Abstract: Genes of the KN1-like homeobox ( KNOX ) class 1 encode transcription factors involved in shoot apical meristem development and maintenance .
We studied the subcellular localization of Green Fluorescent Protein-tagged rice KNOX proteins ( Oskn1-3 ) after particle bombardment of onion and rice cells and after transformation of Arabidopsis and rice with constitutive and inducible expression constructs .
In all test systems , the three rice KNOX proteins showed nuclear and cytoplasmic localization patterns .
However , Oskn1 additionally showed in some cells a distribution over punctae moving randomly in the cytosol .
Use of an inducible expression system indicated a nuclear presence of Oskn1 in cells of the shoot apical meristem and post-transcriptional down-regulation in early leaf primordia .
Arabidopsis and rice test systems were used to study effects of plant hormones and auxin transport inhibition on KNOX protein localization .
Application of GA3 or 1-NAA shifted protein localization completely to the cytoplasm and resulted in loss of the punctae formed by Oskn1 .
Conversely , NPA application induced a complete nuclear localization of the KNOX proteins .
To study intercellular movement of the KNOX proteins we set up a novel co-bombardment assay in which trafficking of untagged KNOX proteins was visualized through the co-trafficking of green fluorescent or blue fluorescent marker proteins .
In multiple independent experiments Oskn1 trafficked more extensively to neighboring cells than Oskn2 and Oskn3 .
Differences in the localization and trafficking properties of Oskn1 , Oskn2 and Oskn3 correlate with differences in mRNA localization patterns and functional differences between the rice KNOX genes and their putative orthologues from other species . | Matching Sentences:
[ Sen. 10, subscore: 1.00 ]: In multiple independent experiments Oskn1 trafficked more extensively to neighboring cells than Oskn2 and Oskn3 .
[ Sen. 11, subscore: 1.00 ]: Differences in the localization and trafficking properties of Oskn1 , Oskn2 and Oskn3 correlate with differences in mRNA localization patterns and functional differences between the rice KNOX genes and their putative orthologues from other species .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis .
| | Author: Postma-Haarsma AD Verwoert II Stronk OP Koster J Lamers GE Hoge JH Meijer AH .
| | Journal: Plant Mol . Biol . Citation: V : 39 ( 2 ) P : 257-71 Year: 1999 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub10080693 Accession (PMID): 10080693 | | Abstract: For identification of genes involved in embryogenesis in the model cereal rice , we have constructed a collection of cDNA libraries of well-defined stages of embryo development before , during and after organ differentiation .
Here , we focus on the possible role of KNOX ( maize Knotted1-like ) class homeobox genes in regulation of rice embryogenesis .
Three types of KNOX clones were identified in libraries of early zygotic embryos .
Two of these , Oskn2 and Oskn3 , encode newly described KNOX genes , whereas the third ( Oskn1 ) corresponds to the previously described OSH1 gene .
In situ hybridizations showed that during the early stages of embryo development , all three KNOX genes are expressed in the region where the shoot apical meristem ( SAM ) is organizing , suggesting that these genes are involved in regulating SAM formation .
Whereas OSH1 was previously proposed to function also in SAM maintenance , Oskn3 may be involved in patterning organ positions , as its expression was found to mark the boundaries of different embryonic organs following SAM formation .
The expression pattern of Oskn2 suggested an additional role in scutellum and epiblast development .
Transgenic expression of Oskn2 and Oskn3 in tobacco further supported their involvement in cell fate determination , like previously reported for Knotted1 and OSH1 ectopic expression .
Whereas Oskn3 transformants showed the most pronounced phenotypic effects during vegetative development , Oskn2 transformants showed relatively mild alterations in the vegetative phase but a more severely affected flower morphology .
The observation that the KNOX genes produce similar though distinct phenotypic reponses in tobacco , indicates that their gene products act on overlapping but different sets of target genes , or that cell-type specific factors determine their precise action . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice .
| | Author: Postma-Haarsma AD Rueb S Scarpella E den Besten W Hoge JH Meijer AH .
| | Journal: Plant Mol . Biol . Citation: V : 48 ( 4 ) P : 423-41 Year: 2002 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub11908517 Accession (PMID): 11908517 | | Abstract: Plant homeobox genes of the class 1 knox ( knotted1-like ) type are involved in the regulation of shoot apical meristem formation and function .
Their expression generally occurs either throughout the meristem or specifically at the lateral organ boundaries .
Down-regulation in the organ primordia is tightly controlled and misexpression in leaves leads to a perturbed development .
Here , the transcriptional control of two rice knox genes , Oskn2 and Oskn3 , was addressed , showing that the promoter sequences of both genes mediate the initial down-regulation during lateral organ formation , but are insufficient to keep expression in lateral organs stably off .
Therefore , maintenance of the repressed state requires control elements outside the promoter regions .
Ectopic expression of Oskn2 or Oskn3 induced similar defects in panicle branching . internode elongation and leaf patterning .
However , small differences suggested that their target gene specificities are not completely overlapping .
This was supported by the observation that Oskn3 protein but not Oskn2 could interact with two reported recognition sequences of a KNOX protein from barley .
Finally , protein-protein interactions may contribute to the functioning of KNOX proteins , as the ability of Oskn3 and Oskn2 to form heterodimers could be demonstrated . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Developmental regulation and downstream effects of the knox class homeobox genes Oskn2 and Oskn3 from rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
