| 14 matches found in 4 documents. Search time: 0.1 seconds. |
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Score: 8.00 |
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| Title: A Ds-insertion mutant of OSH6 ( Oryza sativa Homeobox 6 ) exhibits outgrowth of vestigial leaf-like structures , bracts , in rice .
| | Author: Park SH Kim CM Je BI Park SH Park SJ Piao HL Xuan YH Choe MS Satoh K Kikuchi S Lee KH Cha YS Ahn BO Ji HS Yun DW Lee MC Suh SC Eun MY Han CD | | Journal: Planta Citation: V : 227 P : 112 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17624547 Accession (PMID): 17624547 | | Abstract: OSH6 ( Oryza sativa Homeobox6 ) is an ortholog of lg3 ( Liguleless3 ) in maize .
We generated a novel allele , termed OSH6-Ds , by inserting a defective Ds element into the third exon of OSH6 , which resulted in a truncated OSH6 mRNA .
The truncated mRNA was expressed ectopically in leaf it issues and encoded the N-terminal region of OSH6 , which includes the KNOX1 and partial KNOX2 subdomains .
This recessive mutant showed outgrowth of bracts or produced leaves at the basal node of the panicle .
These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a "blade to sheath transformation" phenotype at the midrib region of leaves , similar to that seen in dominant Lg3 mutants .
Expression of a similar truncated OSH6 cDNA from the 35S promoter ( 35S : : DeltaOSH6 ) confirmed that the ectopic expression of this product was responsible for the aberrant bract development .
These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation , especially at the basal nodes of panicles .
| Matching Sentences:
[ Sen. 2, subscore: 3.00 ]: We generated a novel allele , termed OSH6-Ds , by inserting a defective Ds element into the third exon of OSH6 , which resulted in a truncated OSH6 mRNA .
[ Sen. 1, subscore: 1.00 ]: OSH6 ( Oryza sativa Homeobox6 ) is an ortholog of lg3 ( Liguleless3 ) in maize .
[ Sen. 3, subscore: 1.00 ]: The truncated mRNA was expressed ectopically in leaf it issues and encoded the N-terminal region of OSH6 , which includes the KNOX1 and partial KNOX2 subdomains .
[ Sen. 5, subscore: 1.00 ]: These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a "blade to sheath transformation" phenotype at the midrib region of leaves , similar to that seen in dominant Lg3 mutants .
[ Sen. 6, subscore: 1.00 ]: Expression of a similar truncated OSH6 cDNA from the 35S promoter ( 35S : : DeltaOSH6 ) confirmed that the ectopic expression of this product was responsible for the aberrant bract development .
[ Sen. 7, subscore: 1.00 ]: These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation , especially at the basal nodes of panicles .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 6.00 |
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| Title: A novel alkaline alpha-galactosidase gene is involved in rice leaf senescence .
| | Author: Lee RH Lin MC Chen SC .
| | Journal: Plant Mol . Biol . Citation: V : 55 ( 2 ) P : 281-95 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15604681 Accession (PMID): 15604681 | | Abstract: We previously isolated and identified numerous senescence-associated genes ( SAGs ) in rice leaves .
Here we characterized the structure and function of an SAG Osh69 encoding alkaline alpha-galactosidase that belongs to a novel family of glycosyl hydrolases .
Osh69 is a single-copy gene composed of 13 exons located on rice chromosome 8 .
The expression level of Osh69 is not only up-regulated during natural leaf senescence but also induced rapidly by darkness , hormones ( methyl jasmonic acid , salicylic acid ) , and stresses ( H2O2 and wounding ) .
The recombinant Osh69 protein over-expressed in Escherichia coli has displayed optimal alpha-galactosidase activity at pH 8 . 0 .
The enzyme showed good hydrolytic activities towards alpha-1 , 6-galactosyl oligosaccharides and galactolipid digalactosyl diacylglycerol .
Immunoelectron microscopic analysis demonstrates that Osh69 is specifically localized in the chloroplasts of senescing leaves .
These findings strongly suggest an important role for Osh69 in the degradation of chloroplast galactolipids during leaf senescence . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Here we characterized the structure and function of an SAG Osh69 encoding alkaline alpha-galactosidase that belongs to a novel family of glycosyl hydrolases .
[ Sen. 3, subscore: 1.00 ]: Osh69 is a single-copy gene composed of 13 exons located on rice chromosome 8 .
[ Sen. 4, subscore: 1.00 ]: The expression level of Osh69 is not only up-regulated during natural leaf senescence but also induced rapidly by darkness , hormones ( methyl jasmonic acid , salicylic acid ) , and stresses ( H2O2 and wounding ) .
[ Sen. 5, subscore: 1.00 ]: The recombinant Osh69 protein over-expressed in Escherichia coli has displayed optimal alpha-galactosidase activity at pH 8 . 0 .
[ Sen. 7, subscore: 1.00 ]: Immunoelectron microscopic analysis demonstrates that Osh69 is specifically localized in the chloroplasts of senescing leaves .
[ Sen. 8, subscore: 1.00 ]: These findings strongly suggest an important role for Osh69 in the degradation of chloroplast galactolipids during leaf senescence .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Regulation and a conserved intron sequence of liguleless3/4 knox class-I homeobox genes in grasses .
| | Author: Bauer P Lubkowitz M Tyers R Nemoto K Meeley RB Goff SA Freeling M | | Journal: Planta Citation: V : 219 ( 2 ) P : 359-68 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15034715 Accession (PMID): 15034715 | | Abstract: The nine class-I maize ( Zea mays L ) knox genes are putative transcription factors normally expressed in shoot apices , but not in leaves . knotted1 ( kn1 ) seems to function in shoot apical meristem maintenance , and rough sheath1 ( rs1 ) -like genes may act in internode elongation .
The function of liguleless3 ( lg3 ) -type genes is still unknown .
Here , we characterized lg3 as well as the two most closely related genes liguleless4a ( lg4a , formerly knox11 ) and liguleless4b ( lg4b , formerly knox5 ) .
We termed this subclass of knox genes lg3/4 genes .
We studied the expression patterns of lg3/4 genes and compared their sequences .
We obtained knockout mutants of lg3 by finding Mu transposon insertions into exons .
Our results show that lg3 was not essential for plant development , and that lg4a and lg4b were likely to encode the redundant function .
In addition , lg4a but not lg4b was ectopically expressed in the Lg4-O mutant , suggesting that this mutant was affected at the lg4a locus .
We found that the lg3 gene was unique among knox genes as it was co-induced in the leaves of leaf mutants that ectopically expressed knox genes in the leaves .
The leaf phenotype expressed in the dominant Rs1-O mutant was not altered when lg3 function was removed using the knockout .
Genomic sequence comparisons of lg3 , lg4a and lg4b from maize and the two homologous genes , osh6 and osh71 , from rice revealed a 14-bp phylogenetic footprint in intron II .
This sequence was conserved in nucleotide composition , position and polarity in the lg3/4 genes of divergent grasses representing six Gramineae subfamilies .
In an independent experiment , this same conserved sequence was found in a yeast reverse one-hybrid screen for putative binding sites of the LG3 homeodomain protein .
Distribution of this 14-bp sequence was examined within the public rice database .
The possible function of this sequence in regulation of lg3/4 genes is discussed .
| Matching Sentences:
[ Sen. 11, subscore: 1.00 ]: Genomic sequence comparisons of lg3 , lg4a and lg4b from maize and the two homologous genes , osh6 and osh71 , from rice revealed a 14-bp phylogenetic footprint in intron II .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: A Ds-insertion mutant of OSH6 ( Oryza sativa Homeobox 6 ) exhibits outgrowth of vestigial leaf-like structures , bracts , in rice .
| | Author: Park SH Kim CM Je BI Park SH Park SJ Piao HL Xuan YH Choe MS Satoh K Kikuchi S Lee KH Cha YS Ahn BO Ji HS Yun DW Lee MC Suh SC Eun MY Han CD | | Journal: Planta Citation: V : 227 P : 112 Year: 2007 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub17624547 Accession (PMID): 17624547 | | Abstract: OSH6 ( Oryza sativa Homeobox6 ) is an ortholog of lg3 ( Liguleless3 ) in maize .
We generated a novel allele , termed OSH6-Ds , by inserting a defective Ds element into the third exon of OSH6 , which resulted in a truncated OSH6 mRNA .
The truncated mRNA was expressed ectopically in leaf it issues and encoded the N-terminal region of OSH6 , which includes the KNOX1 and partial KNOX2 subdomains .
This recessive mutant showed outgrowth of bracts or produced leaves at the basal node of the panicle .
These phenotypes distinguished it from the OSH6 transgene whose ectopic expression led to a "blade to sheath transformation" phenotype at the midrib region of leaves , similar to that seen in dominant Lg3 mutants .
Expression of a similar truncated OSH6 cDNA from the 35S promoter ( 35S : : DeltaOSH6 ) confirmed that the ectopic expression of this product was responsible for the aberrant bract development .
These data suggest that OSH6-Ds interferes with a developmental mechanism involved in bract differentiation , especially at the basal nodes of panicles .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: A Ds-insertion mutant of OSH6 ( Oryza sativa Homeobox 6 ) exhibits outgrowth of vestigial leaf-like structures , bracts , in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
