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| Title: Cloning and characterization of OsORC2 , a new member of rice origin recognition complex | | Author: Li KG Yang JS Attia K Su W He GM Qian XY .
| | Journal: Biotechnol . Lett . Citation: V : 27 ( 18 ) P : 1355-9 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16215849 Accession (PMID): 16215849 | | Abstract: In eukaryotic cells , the origin recognition complex ( ORC ) governs the initiation site of DNA replication and formation of the prereplication complex The isolation , characterization and it issue-specific expression of a putative ORC subunit 2 ( OsORC2 ) in Oryza sativa is described here .
A novel cDNA fragment encoding rice ORC2 was isolated by screening the subtractive library , which had a higher expression level in inflorescence meristem than in shoot apical meristem .
The full-length cDNA of rice ORC2 was obtained by the method of rapid amplification of cDNA ends , which contained an 1140 bp open reading frame encoding a 379 amino acid polypeptide .
Sequence alignment shows that there is a high homology between the deduced amino sequence of OsORC2 and maize ORC2 ( 85% ) .
The it issue-specific expression pattern of OsORC2 reveals that it is abundant in roots , seedling and inflorescence meristem , while its expression level is much lower in mature leaves and shoot . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: A novel cDNA fragment encoding rice ORC2 was isolated by screening the subtractive library , which had a higher expression level in inflorescence meristem than in shoot apical meristem .
[ Sen. 3, subscore: 1.00 ]: The full-length cDNA of rice ORC2 was obtained by the method of rapid amplification of cDNA ends , which contained an 1140 bp open reading frame encoding a 379 amino acid polypeptide .
[ Sen. 4, subscore: 1.00 ]: Sequence alignment shows that there is a high homology between the deduced amino sequence of OsORC2 and maize ORC2 ( 85% ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
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| Title: Putative subunits of the maize origin of replication recognition complex ZmORC1-ZmORC5 .
| | Author: Witmer X Alvarez-Venegas R San-Miguel P Danilevskaya O Avramova Z | | Journal: Nucleic Acids Res .
Citation: V : 31 ( 2 ) P : 619-28 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12527770 Accession (PMID): 12527770 | | Abstract: The finding in animal species of complexes homologous to the products of six Saccharomyces cerevisiae genes , origin of replication recognition complex ( ORC ) , has suggested that ORC-related mechanisms have been conserved in all eukaryotes .
In plants , however , the only cloned putative homologs of ORC subunits are the Arabidopsis ORC2 and the rice ORC1 .
Homologs of other subunits of plant origin have not been cloned and characterized .
A striking observation was the absence from the Arabidopsis genome of an obvious candidate gene-homolog of ORC4 .
This fact raised compelling questions of whether plants , in general , and Arabidopsis , in particular , may have lost the ORC4 gene , whether ORC-homologous subunits function within a complex in plants , whether an ORC complex may form and function without an ORC4 subunit , whether a functional ( but not sequence ) protein homolog may have taken up the role of ORC4 in Arabidopsis , and whether lack of ORC4 is a plant feature , in general Here , we report the first cloned and molecularly characterized five genes coding for the maize putative homologs of ORC subunits ZmORC1 , ZmORC2 , ZmORC3 , ZmORC4 and ZmORC5 .
Their expression profiles in it issues with different cell-dividing activities are compatible with a role in DNA replication .
Based on the potential of ORC-homologous maize proteins to bind each other in yeast , we propose a model for their possible assembly within a maize ORC .
The isolation and molecular characterization of an ORC4-homologous gene from maize argues that , in its evolution , Arabidopsis may have lost the homologous ORC4 gene .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In plants , however , the only cloned putative homologs of ORC subunits are the Arabidopsis ORC2 and the rice ORC1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
