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| Title: Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice .
| | Author: Tani T Sobajima H Okada K Chujo T Arimura S Tsutsumi N Nishimura M Seto H Nojiri H Yamane H | | Journal: Planta Citation: V : 227 P : 517-26 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17938955 Accession (PMID): 17938955 | | Abstract: Enzyme 12-oxophytodienoate ( OPDA ) reductase ( EC1 . 3 . 1 . 42 ) , which is involved in the biosynthesis of jasmonic acid ( JA ) , catalyses the reduction of 10 , 11-double bonds of OPDA to yield 3-oxo-2- ( 2-pentenyl ) -cyclopentane-1-octanoic acid ( OPC-8 : 0 ) .
The rice OsOPR1 gene encodes OPDA reductase ( OPR ) converting ( - ) -cis-OPDA preferentially , rather than ( + ) -cis-OPDA , a natural precursor of JA .
Here , we provide evidence that an OPR family gene in rice chromosome 8 , designated OsOPR7 , encodes the enzyme involved in the JA biosynthesis .
Recombinant OsOPR7-His protein efficiently catalysed the reduction of both enantiomers of cis-OPDA , similar to the OPR3 protein in Arabidopsis thaliana ( L ) Heynh .
The expression of OsOPR7 mRNA was induced and reached maximum levels within 0 . 5 h of mechanical wounding and drought stress , and the endogenous JA level started to increase in accordance with the increase in OsOPR7 expression .
The GFP-OsOPR7 fusion protein was detected exclusively in peroxisomes in onion epidermal cells .
Furthermore , complementation analysis using an Arabidopsis opr3 mutant indicated that the OsOPR7 gene , but not OsOPR1 , was able to complement the phenotypes of male sterility in the mutant caused by JA deficiency , and that JA production in the opr3 mutant was also restored by the expression of the OsOPR7 gene .
We conclude that the OsOPR7 gene encodes the enzyme catalysing the reduction of natural ( + ) -cis-OPDA for the JA biosynthesis in rice .
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[ Sen. 7, subscore: 2.00 ]: Furthermore , complementation analysis using an Arabidopsis opr3 mutant indicated that the OsOPR7 gene , but not OsOPR1 , was able to complement the phenotypes of male sterility in the mutant caused by JA deficiency , and that JA production in the opr3 mutant was also restored by the expression of the OsOPR7 gene .
[ Sen. 4, subscore: 1.00 ]: Recombinant OsOPR7-His protein efficiently catalysed the reduction of both enantiomers of cis-OPDA , similar to the OPR3 protein in Arabidopsis thaliana ( L ) Heynh .
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