Score: 8.00 |
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| Title: Molecular characterization of ONAC300 , a novel NAC gene specifically expressed at early stages in various developing it issues of rice .
| | Author: Kusano H Asano T Shimada H Kadowaki K | | Journal: Mol . Genet . Genomics Citation: V : 272 ( 6 ) P : 616-26 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15654622 Accession (PMID): 15654622 | | Abstract: Members of the plant-specific gene family referred to as the NAC family ( for NAM-ATAF-CUC-related ) are involved in various functions including the regulation of plant development .
However , no detailed molecular characterization of any member of the NAC family has yet been reported from monocots .
Here , we report such a characterization of ONAC300 , a novel NAC-family gene identified using a cDNA cloned from microdissected phloem cells of rice .
The predicted ONAC300 protein sequence falls into the NAM subgroup , which also contains the proteins CUC1 and CUC2 from Arabidopsis , CUP from snapdragon , CmNACP from pumpkin and NAM from petunia .
High levels of ONAC300 mRNA were detected by in situ hybridization in developing shoot apical meristem ( SAM ) and in the associated young leaves .
The use of an ONAC300 : : GUS reporter gene revealed that the ONAC300 promoter was expressed predominantly in developing vascular it issues of the leaves and roots .
The construct was also expressed in anther filaments , rachis and carpel styles .
RT-PCR analysis further revealed that the levels of ONAC300 transcripts were higher in leaves , roots and culms than in panicles .
The observed expression pattern of ONAC300 is quite different from those of the dicot NAC genes previously reported .
Thus , ONAC300 is a novel member of the NAC family which is expressed at very early developmental stages in the shoot , root and flower , as well as in the mature phloem of vascular it issues in rice . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: The use of an ONAC300 : : GUS reporter gene revealed that the ONAC300 promoter was expressed predominantly in developing vascular it issues of the leaves and roots .
[ Sen. 3, subscore: 1.00 ]: Here , we report such a characterization of ONAC300 , a novel NAC-family gene identified using a cDNA cloned from microdissected phloem cells of rice .
[ Sen. 4, subscore: 1.00 ]: The predicted ONAC300 protein sequence falls into the NAM subgroup , which also contains the proteins CUC1 and CUC2 from Arabidopsis , CUP from snapdragon , CmNACP from pumpkin and NAM from petunia .
[ Sen. 5, subscore: 1.00 ]: High levels of ONAC300 mRNA were detected by in situ hybridization in developing shoot apical meristem ( SAM ) and in the associated young leaves .
[ Sen. 8, subscore: 1.00 ]: RT-PCR analysis further revealed that the levels of ONAC300 transcripts were higher in leaves , roots and culms than in panicles .
[ Sen. 9, subscore: 1.00 ]: The observed expression pattern of ONAC300 is quite different from those of the dicot NAC genes previously reported .
[ Sen. 10, subscore: 1.00 ]: Thus , ONAC300 is a novel member of the NAC family which is expressed at very early developmental stages in the shoot , root and flower , as well as in the mature phloem of vascular it issues in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Molecular characterization of ONAC300 , a novel NAC gene specifically expressed at early stages in various developing it issues of rice .
| | Author: Kusano H Asano T Shimada H Kadowaki K | | Journal: Mol . Genet . Genomics Citation: V : 272 ( 6 ) P : 616-26 Year: 2005 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub15654622 Accession (PMID): 15654622 | | Abstract: Members of the plant-specific gene family referred to as the NAC family ( for NAM-ATAF-CUC-related ) are involved in various functions including the regulation of plant development .
However , no detailed molecular characterization of any member of the NAC family has yet been reported from monocots .
Here , we report such a characterization of ONAC300 , a novel NAC-family gene identified using a cDNA cloned from microdissected phloem cells of rice .
The predicted ONAC300 protein sequence falls into the NAM subgroup , which also contains the proteins CUC1 and CUC2 from Arabidopsis , CUP from snapdragon , CmNACP from pumpkin and NAM from petunia .
High levels of ONAC300 mRNA were detected by in situ hybridization in developing shoot apical meristem ( SAM ) and in the associated young leaves .
The use of an ONAC300 : : GUS reporter gene revealed that the ONAC300 promoter was expressed predominantly in developing vascular it issues of the leaves and roots .
The construct was also expressed in anther filaments , rachis and carpel styles .
RT-PCR analysis further revealed that the levels of ONAC300 transcripts were higher in leaves , roots and culms than in panicles .
The observed expression pattern of ONAC300 is quite different from those of the dicot NAC genes previously reported .
Thus , ONAC300 is a novel member of the NAC family which is expressed at very early developmental stages in the shoot , root and flower , as well as in the mature phloem of vascular it issues in rice . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Molecular characterization of ONAC300 , a novel NAC gene specifically expressed at early stages in various developing it issues of rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
