| 17 matches found in 4 documents. Search time: 0.126 seconds. |
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Score: 12.00 |
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| Title: Rice NON-YELLOW COLORING1 Is Involved in Light-Harvesting Complex II and Grana Degradation during Leaf Senescence .
| | Author: Kusaba M Ito H Morita R Iida S Sato Y Fujimoto M Kawasaki S Tanaka R Hirochika H Nishimura M Tanaka A | | Journal: Citation: V : ( ) P : Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17416733 Accession (PMID): 17416733 | | Abstract: Chlorophyll degradation is an aspect of leaf senescence , which is an active process to salvage nutrients from old it issues . non-yellow coloring1 ( nyc1 ) is a rice ( Oryza sativa ) stay-green mutant in which chlorophyll degradation during senescence is impaired .
Pigment analysis revealed that degradation of not only chlorophylls but also light-harvesting complex II ( LHCII ) -bound carotenoids was repressed in nyc1 , in which most LHCII isoforms were selectively retained during senescence .
Ultrastructural analysis of nyc1 chloroplasts revealed that large and thick grana were present even in the late stage of senescence , suggesting that degradation of LHCII is required for the proper degeneration of thylakoid membranes .
Map-based cloning of NYC1 revealed that it encodes a chloroplast-localized short-chain dehydrogenase/reductase ( SDR ) with three transmembrane domains .
The predicted structure of the NYC1 protein and the phenotype of the nyc1 mutant suggest the possibility that NYC1 is a chlorophyll b reductase .
Although we were unable to detect the chlorophyll b reductase activity of NYC1 , NOL ( for NYC1-like ) , a protein closely related to NYC1 in rice , showed chlorophyll b reductase activity in vitro .
We suggest that NYC1 and NOL encode chlorophyll b reductases with divergent functions .
Our data collectively suggest that the identified SDR protein NYC1 plays essential roles in the regulation of LHCII and thylakoid membrane degradation during senescence .
| Matching Sentences:
[ Sen. 5, subscore: 3.00 ]: The predicted structure of the NYC1 protein and the phenotype of the nyc1 mutant suggest the possibility that NYC1 is a chlorophyll b reductase .
[ Sen. 6, subscore: 3.00 ]: Although we were unable to detect the chlorophyll b reductase activity of NYC1 , NOL ( for NYC1-like ) , a protein closely related to NYC1 in rice , showed chlorophyll b reductase activity in vitro .
[ Sen. 1, subscore: 1.00 ]: Chlorophyll degradation is an aspect of leaf senescence , which is an active process to salvage nutrients from old it issues . non-yellow coloring1 ( nyc1 ) is a rice ( Oryza sativa ) stay-green mutant in which chlorophyll degradation during senescence is impaired .
[ Sen. 2, subscore: 1.00 ]: Pigment analysis revealed that degradation of not only chlorophylls but also light-harvesting complex II ( LHCII ) -bound carotenoids was repressed in nyc1 , in which most LHCII isoforms were selectively retained during senescence .
[ Sen. 3, subscore: 1.00 ]: Ultrastructural analysis of nyc1 chloroplasts revealed that large and thick grana were present even in the late stage of senescence , suggesting that degradation of LHCII is required for the proper degeneration of thylakoid membranes .
[ Sen. 4, subscore: 1.00 ]: Map-based cloning of NYC1 revealed that it encodes a chloroplast-localized short-chain dehydrogenase/reductase ( SDR ) with three transmembrane domains .
[ Sen. 7, subscore: 1.00 ]: We suggest that NYC1 and NOL encode chlorophyll b reductases with divergent functions .
[ Sen. 8, subscore: 1.00 ]: Our data collectively suggest that the identified SDR protein NYC1 plays essential roles in the regulation of LHCII and thylakoid membrane degradation during senescence .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 8.00 |
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| Title: Two short-chain dehydrogenase/reductases , NON-YELLOW COLORING 1 and NYC1-LIKE , are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice .
| | Author: Sato Y Morita R Katsuma S Nishimura M Tanaka A Kusaba M | | Journal: Plant J Citation: V : 57 P : 120-31 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18778405 Accession (PMID): 18778405 | | Abstract: Yellowing , which is related to the degradation of chlorophyll and chlorophyll-protein complexes , is a notable phenomenon during leaf senescence .
NON-YELLOW COLORING 1 ( NYC1 ) in rice encodes a membrane-localized short-chain dehydrogenase/reductase ( SDR ) that is thought to represent a chlorophyll b reductase necessary for catalyzing the first step of chlorophyll b degradation .
Analysis of the nyc1 mutant , which shows the stay-green phenotype , revealed that chlorophyll b degradation is required for the degradation of light-harvesting complex II and thylakoid grana in leaf senescence .
Phylogenetic analysis further revealed the existence of NYC1-LIKE ( NOL ) as the most closely related protein to NYC1 .
In the present paper , the nol mutant in rice was also found to show a stay-green phenotype very similar to that of the nyc1 mutant , ie the degradation of chlorophyll b was severely inhibited and light-harvesting complex II was selectively retained during senescence , resulting in the retention of thylakoid grana even at a late stage of senescence .
The nyc1 nol double mutant did not show prominent enhancement of inhibition of chlorophyll degradation .
NOL was localized on the stromal side of the thylakoid membrane despite the lack of a transmembrane domain .
Immunoprecipitation analysis revealed that NOL and NYC1 interact physically in vitro .
These observations suggest that NOL and NYC1 are co-localized in the thylakoid membrane and act in the form of a complex as a chlorophyll b reductase in rice .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Phylogenetic analysis further revealed the existence of NYC1-LIKE ( NOL ) as the most closely related protein to NYC1 .
[ Sen. 2, subscore: 1.00 ]: NON-YELLOW COLORING 1 ( NYC1 ) in rice encodes a membrane-localized short-chain dehydrogenase/reductase ( SDR ) that is thought to represent a chlorophyll b reductase necessary for catalyzing the first step of chlorophyll b degradation .
[ Sen. 3, subscore: 1.00 ]: Analysis of the nyc1 mutant , which shows the stay-green phenotype , revealed that chlorophyll b degradation is required for the degradation of light-harvesting complex II and thylakoid grana in leaf senescence .
[ Sen. 5, subscore: 1.00 ]: In the present paper , the nol mutant in rice was also found to show a stay-green phenotype very similar to that of the nyc1 mutant , ie the degradation of chlorophyll b was severely inhibited and light-harvesting complex II was selectively retained during senescence , resulting in the retention of thylakoid grana even at a late stage of senescence .
[ Sen. 6, subscore: 1.00 ]: The nyc1 nol double mutant did not show prominent enhancement of inhibition of chlorophyll degradation .
[ Sen. 8, subscore: 1.00 ]: Immunoprecipitation analysis revealed that NOL and NYC1 interact physically in vitro .
[ Sen. 9, subscore: 1.00 ]: These observations suggest that NOL and NYC1 are co-localized in the thylakoid membrane and act in the form of a complex as a chlorophyll b reductase in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Mendels green cotyledon gene encodes a positive regulator of the chlorophyll-degrading pathway .
| | Author: Sato Y Morita R Nishimura M Yamaguchi H Kusaba M | | Journal: Proc Natl Acad Sci U S A Citation: V : 104 P : 14169-74 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17709752 Accession (PMID): 17709752 | | Abstract: Mutants that retain greenness of leaves during senescence are known as "stay-green" mutants .
The most famous stay-green mutant is Mendels green cotyledon pea , one of the mutants used in determining the law of genetics .
Pea plants homozygous for this recessive mutation ( known as i at present ) retain greenness of the cotyledon during seed maturation and of leaves during senescence .
We found tight linkage between the I locus and stay-green gene originally found in rice , SGR .
Molecular analysis of three i alleles including one with no SGR expression confirmed that the I gene encodes SGR in pea .
Functional analysis of sgr mutants in pea and rice further revealed that leaf functionality is lowered despite a high chlorophyll a ( Chl a ) and chlorophyll b ( Chl b ) content in the late stage of senescence , suggesting that SGR is primarily involved in Chl degradation .
Consistent with this observation , a wide range of Chl-protein complexes , but not the ribulose-1 , 5-bisphosphate carboxylase/oxygenase ( Rubisco ) large subunit , were shown to be more stable in sgr than wild-type plants .
The expression of OsCHL and NYC1 , which encode the first enzymes in the degrading pathways of Chl a and Chl b , respectively , was not affected by sgr in rice .
The results suggest that SGR might be involved in activation of the Chl-degrading pathway during leaf senescence through translational or posttranslational regulation of Chl-degrading enzymes .
| Matching Sentences:
[ Sen. 8, subscore: 1.00 ]: The expression of OsCHL and NYC1 , which encode the first enzymes in the degrading pathways of Chl a and Chl b , respectively , was not affected by sgr in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Two short-chain dehydrogenase/reductases , NON-YELLOW COLORING 1 and NYC1-LIKE , are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice .
| | Author: Sato Y Morita R Katsuma S Nishimura M Tanaka A Kusaba M | | Journal: Plant J Citation: V : 57 P : 120-31 Year: 2009 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18778405 Accession (PMID): 18778405 | | Abstract: Yellowing , which is related to the degradation of chlorophyll and chlorophyll-protein complexes , is a notable phenomenon during leaf senescence .
NON-YELLOW COLORING 1 ( NYC1 ) in rice encodes a membrane-localized short-chain dehydrogenase/reductase ( SDR ) that is thought to represent a chlorophyll b reductase necessary for catalyzing the first step of chlorophyll b degradation .
Analysis of the nyc1 mutant , which shows the stay-green phenotype , revealed that chlorophyll b degradation is required for the degradation of light-harvesting complex II and thylakoid grana in leaf senescence .
Phylogenetic analysis further revealed the existence of NYC1-LIKE ( NOL ) as the most closely related protein to NYC1 .
In the present paper , the nol mutant in rice was also found to show a stay-green phenotype very similar to that of the nyc1 mutant , ie the degradation of chlorophyll b was severely inhibited and light-harvesting complex II was selectively retained during senescence , resulting in the retention of thylakoid grana even at a late stage of senescence .
The nyc1 nol double mutant did not show prominent enhancement of inhibition of chlorophyll degradation .
NOL was localized on the stromal side of the thylakoid membrane despite the lack of a transmembrane domain .
Immunoprecipitation analysis revealed that NOL and NYC1 interact physically in vitro .
These observations suggest that NOL and NYC1 are co-localized in the thylakoid membrane and act in the form of a complex as a chlorophyll b reductase in rice .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Two short-chain dehydrogenase/reductases , NON-YELLOW COLORING 1 and NYC1-LIKE , are required for chlorophyll b and light-harvesting complex II degradation during senescence in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
