| 5 matches found in 3 documents. Search time: 0.218 seconds. |
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Score: 3.00 |
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| Title: Cloning and characterization of the OsNramp family from Oryza sativa , a new family of membrane proteins possibly implicated in the transport of metal ions .
| | Author: Belouchi A Kwan T Gros P | | Journal: Plant Mol . Biol . Citation: V : 33 ( 6 ) P : 1085-92 Year: 1997 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9154989 Accession (PMID): 9154989 | | Abstract: The mammalian Nramp1 protein is an integral membrane protein expressed exclusively in macrophages , where it plays a critical role in the ability of these cells to destroy ingested microbes .
The bactericidal mechanism of action of Nramp1 remains unknown .
We report the identification and characterization of cDNA clones corresponding to three homologues of the mammalian Nramp1 gene from the genome of Oryza sativa , OsNramp1 , OsNramp2 , and OsNramp3 .
These three genes encode a novel group of highly similar hydrophobic polypeptides sharing between 64% and 75% sequence similarity , that show similar hydropathy profiles , and predicted secondary structure , including the same number , position , and sequence characteristics ( including conserved charges ) of transmembrane domains .
Together , these define a highly conserved membrane associated hydrophobic core .
The three plant proteins show a remarkable degree of sequence similarity with their mammalian counterpart ( 60% to 70% similarity ) , including primary and secondary structure elements previously described in ion transporters and channels .
Expression studies in normal plant it issues indicate that while OsNramp1 is expressed primarily in roots , and OsNramp2 is primarily expressed in leaves , OsNramp3 is expressed in both it issues .
The recent discovery that the yeast Nramp homologue SMF1 functions as a manganese transporter raises the exciting possibility that OsNramp encodes a family of metal ion transporters in plants . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The mammalian Nramp1 protein is an integral membrane protein expressed exclusively in macrophages , where it plays a critical role in the ability of these cells to destroy ingested microbes .
[ Sen. 2, subscore: 1.00 ]: The bactericidal mechanism of action of Nramp1 remains unknown .
[ Sen. 3, subscore: 1.00 ]: We report the identification and characterization of cDNA clones corresponding to three homologues of the mammalian Nramp1 gene from the genome of Oryza sativa , OsNramp1 , OsNramp2 , and OsNramp3 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Involvement of NRAMP1 from Arabidopsis thaliana in iron transport .
| | Author: Curie C Alonso JM Le Jean M Ecker JR Briat JF .
| | Journal: Biochem .
J Citation: V : 347 Pt 3 ( ) P : 749-55 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10769179 Accession (PMID): 10769179 | | Abstract: Nramp genes code for a widely distributed class of proteins involved in a variety of processes , ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions , including iron .
In plants , iron transport was shown to require a reduction/Fe ( II ) transport system .
In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes .
Here we report the sequence of five NRAMP proteins from A thaliana .
Sequence comparison suggests that there are two classes of NRAMP proteins in plants : A thaliana ( At ) NRAMP1 and Oriza sativa ( Os ) NRAMP1 and 3 ( two rice isologues ) represent one class , and AtNRAMP2-5 and OsNRAMP2 the other .
AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low and high-affinity iron transports , whereas AtNramp2 and OsNramp2 fail to do so .
In addition , AtNramp1 transcript , but not AtNramp2 transcript , accumulates in response to iron deficiency in roots but not in leaves .
Finally , overexpression of AtNramp1 in transgenic A thaliana plants leads to an increase in plant resistance to toxic iron concentration .
Taken together , these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Sequence comparison suggests that there are two classes of NRAMP proteins in plants : A thaliana ( At ) NRAMP1 and Oriza sativa ( Os ) NRAMP1 and 3 ( two rice isologues ) represent one class , and AtNRAMP2-5 and OsNRAMP2 the other .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Involvement of NRAMP1 from Arabidopsis thaliana in iron transport .
| | Author: Curie C Alonso JM Le Jean M Ecker JR Briat JF .
| | Journal: Biochem .
J Citation: V : 347 Pt 3 ( ) P : 749-55 Year: 2000 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub10769179 Accession (PMID): 10769179 | | Abstract: Nramp genes code for a widely distributed class of proteins involved in a variety of processes , ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions , including iron .
In plants , iron transport was shown to require a reduction/Fe ( II ) transport system .
In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes .
Here we report the sequence of five NRAMP proteins from A thaliana .
Sequence comparison suggests that there are two classes of NRAMP proteins in plants : A thaliana ( At ) NRAMP1 and Oriza sativa ( Os ) NRAMP1 and 3 ( two rice isologues ) represent one class , and AtNRAMP2-5 and OsNRAMP2 the other .
AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low and high-affinity iron transports , whereas AtNramp2 and OsNramp2 fail to do so .
In addition , AtNramp1 transcript , but not AtNramp2 transcript , accumulates in response to iron deficiency in roots but not in leaves .
Finally , overexpression of AtNramp1 in transgenic A thaliana plants leads to an increase in plant resistance to toxic iron concentration .
Taken together , these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Involvement of NRAMP1 from Arabidopsis thaliana in iron transport .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
