| 35 matches found in 13 documents. Search time: 0.17 seconds. |
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Score: 13.00 |
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| Title: A rice transient assay system identifies a novel domain in NRR required for interaction with NH1/OsNPR1 and inhibition of NH1-mediated transcriptional activation .
| | Author: Chern M Bai W Sze-To WH Canlas PE Bartley LE Ronald PC | | Journal: Plant Methods Citation: V : 8 P : 6 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22353606 Accession (PMID): 22353606 | | Abstract: ABSTRACT : BACKGROUND : Arabidopsis NPR1 is a master regulator of systemic acquired resistance .
NPR1 binds to TGA transcription factors and functions as a transcriptional co-activator .
In rice , NH1/OsNPR1 functions to enhance innate immunity .
NRR disrupts NH1 function , when over-expressed .
RESULTS : We have established a rice transient protoplast assay to demonstrate that NH1 is a transcriptional co-activator and that NRR represses NH1-mediated activation .
We identified three NRR homologues ( RH1 , RH2 , and RH3 ) .
RH1 and RH3 , but not RH2 , also effectively repress NH1-mediated transcriptional activation .
NRR , RH1 , RH2 , and RH3 share sequence similarity in a region beyond the previously identified NPR1-interacting domain .
This region is required for strong interaction with NH1 .
A double point mutation , W66A/F70A , in this novel NH1-interacting domain severely reduces interaction with NH1 .
Mutation W66A/F70A also greatly reduces the ability of NRR to repress NH1-mediated activation .
RH2 carries a deviation ( amino acids AV ) in this region as compared to consensus sequences ( amino acids ED ) among NRR , RH1 , and RH3 .
A substitution ( AV to ED ) in RH2 results in strong binding of mutant RH2ED to NH1 and effective repression of NH1-mediated activation .
CONCLUSIONS : The protoplast-based transient system can be used to dissect protein domains associated with their functions .
Our results demonstrate that the ability of NRR and its homologues to repress NH1-mediated transcriptional activation is tightly correlated with their ability to bind to NH1 .
Furthermore , a sequence is identified as a novel NH1-interacting domain .
Importantly , this novel sequence is widely present in plant species , from cereals to castor bean plants , to poplar trees , to Arabidopsis , indicating its significance in plants .
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[ Sen. 5, subscore: 2.00 ]: RESULTS : We have established a rice transient protoplast assay to demonstrate that NH1 is a transcriptional co-activator and that NRR represses NH1-mediated activation .
[ Sen. 10, subscore: 2.00 ]: A double point mutation , W66A/F70A , in this novel NH1-interacting domain severely reduces interaction with NH1 .
[ Sen. 13, subscore: 2.00 ]: A substitution ( AV to ED ) in RH2 results in strong binding of mutant RH2ED to NH1 and effective repression of NH1-mediated activation .
[ Sen. 15, subscore: 2.00 ]: Our results demonstrate that the ability of NRR and its homologues to repress NH1-mediated transcriptional activation is tightly correlated with their ability to bind to NH1 .
[ Sen. 4, subscore: 1.00 ]: NRR disrupts NH1 function , when over-expressed .
[ Sen. 7, subscore: 1.00 ]: RH1 and RH3 , but not RH2 , also effectively repress NH1-mediated transcriptional activation .
[ Sen. 9, subscore: 1.00 ]: This region is required for strong interaction with NH1 .
[ Sen. 11, subscore: 1.00 ]: Mutation W66A/F70A also greatly reduces the ability of NRR to repress NH1-mediated activation .
[ Sen. 16, subscore: 1.00 ]: Furthermore , a sequence is identified as a novel NH1-interacting domain .
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Score: 10.00 |
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| Title: Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light .
| | Author: Chern M Fitzgerald HA Canlas PE Navarre DA Ronald PC .
| | Journal: Mol .
Plant Microbe Interact .
Citation: V : 18 ( 6 ) P : 511-20 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15986920 Accession (PMID): 15986920 | | Abstract: Arabidopsis NPR1/NIM1 is a key regulator of systemic acquired resistance ( SAR ) , which confers lasting broad-spectrum resistance .
Previous reports indicate that rice has a disease-resistance pathway similar to the Arabidopsis SAR pathway .
Here we report the isolation and characterization of a rice NPR1 homologue ( NH1 ) .
Transgenic rice plants overexpressing NH1 ( NH1ox ) acquire high levels of resistance to Xanthomonas oryzae pv . oryzae .
The resistance phenotype is heritable and correlates with the presence of the transgene and reduced bacterial growth .
Northern analysis shows that NH1ox rice spontaneously activates defense genes , contrasting with NPR1-overexpressing Arabidopsis , where defense genes are not activated until induction .
Wild-type NH1 , but not a point mutant corresponding to npr1-1 , interacts strongly with the rice transcription factor rTGA2 . 2 in yeast two-hybrid .
Greenhouse-grown NH1ox plants develop lesion-mimic spots on leaves at preflowering stage although no other developmental effects are observed .
However , when grown in growth chambers ( GCs ) under low light , NH1ox plants are dwarfed , indicating elevated sensitivity to light .
The GC-grown NH1ox plants show much higher salicylic acid ( SA ) levels than the wild type , whereas greenhouse-grown NH1ox plants contain lower SA .
These results indicate that NH1 may be involved in the regulation of SA in response to environmental changes . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Transgenic rice plants overexpressing NH1 ( NH1ox ) acquire high levels of resistance to Xanthomonas oryzae pv . oryzae .
[ Sen. 10, subscore: 2.00 ]: The GC-grown NH1ox plants show much higher salicylic acid ( SA ) levels than the wild type , whereas greenhouse-grown NH1ox plants contain lower SA .
[ Sen. 3, subscore: 1.00 ]: Here we report the isolation and characterization of a rice NPR1 homologue ( NH1 ) .
[ Sen. 6, subscore: 1.00 ]: Northern analysis shows that NH1ox rice spontaneously activates defense genes , contrasting with NPR1-overexpressing Arabidopsis , where defense genes are not activated until induction .
[ Sen. 7, subscore: 1.00 ]: Wild-type NH1 , but not a point mutant corresponding to npr1-1 , interacts strongly with the rice transcription factor rTGA2 . 2 in yeast two-hybrid .
[ Sen. 8, subscore: 1.00 ]: Greenhouse-grown NH1ox plants develop lesion-mimic spots on leaves at preflowering stage although no other developmental effects are observed .
[ Sen. 9, subscore: 1.00 ]: However , when grown in growth chambers ( GCs ) under low light , NH1ox plants are dwarfed , indicating elevated sensitivity to light .
[ Sen. 11, subscore: 1.00 ]: These results indicate that NH1 may be involved in the regulation of SA in response to environmental changes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 4.00 |
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| Title: Rice NRR , a negative regulator of disease resistance , interacts with Arabidopsis NPR1 and rice NH1 .
| | Author: Chern M Canlas PE Fitzgerald HA Ronald PC .
| | Journal: Plant J Citation: V : 43 ( 5 ) P : 623-35 Year: 2005 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16115061 Accession (PMID): 16115061 | | Abstract: Arabidopsis NPR1/NIM1 is a key regulator of systemic acquired resistance ( SAR ) , which confers lasting broad-spectrum resistance .
Over-expression of Arabidopsis NPR1 or the NPR1 homolog 1 ( NH1 ) in rice results in enhanced resistance to the pathogen Xanthomonasoryzae pv . oryzae ( Xoo ) , suggesting the presence of a related defense pathway in rice .
We investigated this pathway in rice by identifying proteins that interact with NH1 .
Here we report the isolation and characterization of a rice cDNA encoding a novel protein , named NRR ( for negative regulator of resistance ) .
NRR interacts with NPR1 in the NPR1-interacting domain ( NI25 ) consisting of 25 amino acids .
NRR also interacts with NH1 ; however , NI25 was not sufficient for a strong interaction , indicating a difference between the rice and the Arabidopsis proteins .
Silencing of NRR in rice had little effect on resistance to Xoo .
When constitutively over-expressed in rice , NRR affected basal resistance , age-related resistance and Xa21-mediated resistance , causing enhanced susceptibility to Xoo .
This phenotype was correlated with elevated NRR mRNA and protein levels and increased Xoo growth .
Over-expression of NRR suppressed the induction of defense-related genes .
NRR : GFP ( green fluorescent protein ) protein was localized to the nucleus , indicating that NRR may act directly to suppress the activation of defense genes .
The fact that NRR compromises Xa21-mediated resistance indicates cross-talk or overlap between NH1 and Xa21-mediated pathways . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Over-expression of Arabidopsis NPR1 or the NPR1 homolog 1 ( NH1 ) in rice results in enhanced resistance to the pathogen Xanthomonasoryzae pv . oryzae ( Xoo ) , suggesting the presence of a related defense pathway in rice .
[ Sen. 3, subscore: 1.00 ]: We investigated this pathway in rice by identifying proteins that interact with NH1 .
[ Sen. 6, subscore: 1.00 ]: NRR also interacts with NH1 ; however , NI25 was not sufficient for a strong interaction , indicating a difference between the rice and the Arabidopsis proteins .
[ Sen. 12, subscore: 1.00 ]: The fact that NRR compromises Xa21-mediated resistance indicates cross-talk or overlap between NH1 and Xa21-mediated pathways .
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Score: 4.00 |
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| Title: Rice Snl6 , a cinnamoyl-CoA reductase-like gene family member , is required for NH1-mediated immunity to Xanthomonas oryzae pv . oryzae .
| | Author: Bart RS Chern M Vega-Sanchez ME Canlas P Ronald PC | | Journal: PLoS Genet Citation: V : 6 P : Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20862311 Accession (PMID): 20862311 | | Abstract: Rice NH1 ( NPR1 homolog 1 ) is a key mediator of innate immunity .
In both plants and animals , the innate immune response is often accompanied by rapid cell death at the site of pathogen infection .
Over-expression of NH1 in rice results in resistance to the bacterial pathogen , Xanthomonas oryzae pv . oryzae ( Xoo ) , constitutive expression of defense related genes and enhanced benzothiadiazole ( BTH ) - mediated cell death .
Here we describe a forward genetic screen that identified a suppressor of NH1-mediated lesion formation and resistance , snl6 .
Comparative genome hybridization and fine mapping rapidly identified the genomic location of the Snl6 gene .
Snl6 is a member of the cinnamoyl-CoA reductase ( CCR ) -like gene family .
We show that Snl6 is required for NH1-mediated resistance to Xoo .
Further , we show that Snl6 is required for pathogenesis-related gene expression .
In contrast to previously described CCR family members , disruption of Snl6 does not result in an obvious morphologic phenotype .
Snl6 mutants have reduced lignin content and increased sugar extractability , an important trait for the production of cellulosic biofuels .
These results suggest the existence of a conserved group of CCR-like genes involved in the defense response , and with the potential to alter lignin content without affecting development .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Rice NH1 ( NPR1 homolog 1 ) is a key mediator of innate immunity .
[ Sen. 3, subscore: 1.00 ]: Over-expression of NH1 in rice results in resistance to the bacterial pathogen , Xanthomonas oryzae pv . oryzae ( Xoo ) , constitutive expression of defense related genes and enhanced benzothiadiazole ( BTH ) - mediated cell death .
[ Sen. 4, subscore: 1.00 ]: Here we describe a forward genetic screen that identified a suppressor of NH1-mediated lesion formation and resistance , snl6 .
[ Sen. 7, subscore: 1.00 ]: We show that Snl6 is required for NH1-mediated resistance to Xoo .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Rice WRKY45 plays a crucial role in benzothiadiazole-inducible blast resistance .
| | Author: Shimono M Sugano S Nakayama A Jiang CJ Ono K Toki S Takatsuji H | | Journal: Plant Cell Citation: V : 19 P : 2064-76 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17601827 Accession (PMID): 17601827 | | Abstract: Benzothiadiazole ( BTH ) is a so-called plant activator and protects plants from diseases by activating the salicylic acid ( SA ) signaling pathway .
By microarray screening , we identified BTH and SA-inducible WRKY transcription factor ( TF ) genes that were upregulated within 3 h after BTH treatment .
Overexpression of one of them , WRKY45 , in rice ( Oryza sativa ) markedly enhanced resistance to rice blast fungus .
RNA interference-mediated knockdown of WRKY45 compromised BTH-inducible resistance to blast disease , indicating that it is essential for BTH-induced defense responses .
In a transient expression system , WRKY45 activated reporter gene transcription through W-boxes .
Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1 , a rice ortholog of Arabidopsis thaliana NPR1 , which distinguishes WRKY45 from known Arabidopsis WRKY TFs .
Two defense-related genes , encoding a glutathione S-transferase and a cytochrome P450 , were found to be regulated downstream of WRKY45 but were not regulated by NH1 , consistent with the apparent independence of the WRKY45 and NH1-dependent pathways .
Defense gene expression in WRKY45-overexpressed rice plants varied with growth conditions , suggesting that some environmental factor ( s ) acts downstream of WRKY45 transcription .
We propose a role for WRKY45 in BTH-induced and SA-mediated defense signaling in rice and its potential utility in improving disease resistance of rice , an importance food resource worldwide .
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[ Sen. 7, subscore: 2.00 ]: Two defense-related genes , encoding a glutathione S-transferase and a cytochrome P450 , were found to be regulated downstream of WRKY45 but were not regulated by NH1 , consistent with the apparent independence of the WRKY45 and NH1-dependent pathways .
[ Sen. 6, subscore: 1.00 ]: Epistasis analysis suggested that WRKY45 acts in the SA signaling pathway independently of NH1 , a rice ortholog of Arabidopsis thaliana NPR1 , which distinguishes WRKY45 from known Arabidopsis WRKY TFs .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
