| 6 matches found in 4 documents. Search time: 0.509 seconds. |
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| Title: Interspecies compatibility of NAS1 gene promoters .
| | Author: Ito S Inoue H Kobayashi T Yoshiba M Mori S Nishizawa N Higuchi K | | Journal: Plant Physiol Biochem Citation: V : 45 P : 270-6 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17524656 Accession (PMID): 17524656 | | Abstract: Nicotianamine and nicotianamine synthase ( NAS ) play key roles in iron nutrition in all higher plants .
However , the mechanism underlying the regulation of NAS expression differs among plant species .
Sequences homologous to iron deficiency-responsive elements ( IDEs ) , ie , cis-acting elements , are found on the promoters of these genes .
We aimed to verify the interspecies compatibility of the Fe-deficiency response of NAS1 genes and understand the universal mechanisms that regulate their expression patterns in higher plants .
Therefore , we introduced the graminaceous ( Hordeum vulgare L and Oryza sativa L ) NAS1 promoter : : GUS into dicots ( Nicotiana tabacum L and Arabidopsis thaliana L ) .
Fe deficiency induced HvNAS1 expression in the shoots and roots when introduced into rice .
HvNAS1 promoter : : GUS and OsNAS1 promoter : : GUS induced strong expression of GUS under Fe-deficient conditions in transformed tobacco In contrast , these promoters only definitely functioned in Arabidopsis transformants .
These results suggest that some Fe nutrition-related trans-factors are not compatible between graminaceous plants and Arabidopsis .
HvNAS1 promoter : : GUS induced GUS activity only in the roots of transformed tobacco under Fe-deficient conditions .
On the other hand , OsNAS1 promoter : : GUS induced GUS activity in both the roots and shoots of transformed tobacco under conditions of Fe deficiency .
In tobacco transformants , the induction of GUS activity was induced earlier in the shoots than roots .
These results suggest that the HvNAS1 and OsNAS1 promoters are compatible with Fe-acquisition-related trans-factors in the roots of tobacco and that the OsNAS1 promoter is also compatible with some shoot-specific Fe deficiency-related trans-factors in tobacco | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: We aimed to verify the interspecies compatibility of the Fe-deficiency response of NAS1 genes and understand the universal mechanisms that regulate their expression patterns in higher plants .
[ Sen. 5, subscore: 1.00 ]: Therefore , we introduced the graminaceous ( Hordeum vulgare L and Oryza sativa L ) NAS1 promoter : : GUS into dicots ( Nicotiana tabacum L and Arabidopsis thaliana L ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Cloning of nicotianamine synthase genes , novel genes involved in the biosynthesis of phytosiderophores .
| | Author: Higuchi K Suzuki K Nakanishi H Yamaguchi H Nishizawa NK Mori S | | Journal: Plant Physiol .
Citation: V : 119 ( 2 ) P : 471-80 Year: 1999 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9952442 Accession (PMID): 9952442 | | Abstract: Nicotianamine synthase ( NAS ) , the key enzyme in the biosynthetic pathway for the mugineic acid family of phytosiderophores , catalyzes the trimerization of S-adenosylmethionine to form one molecule of nicotianamine .
We purified NAS protein and isolated the genes nas1 , nas2 , nas3 , nas4 , nas5-1 , nas5-2 , and nas6 , which encode NAS and NAS-like proteins from Fe-deficient barley ( Hordeum vulgare L cv Ehimehadaka no 1 ) roots .
Escherichia coli expressing nas1 showed NAS activity , confirming that this gene encodes a functional NAS .
Expression of nas genes as determined by northern-blot analysis was induced by Fe deficiency and was root specific .
The NAS genes form a multigene family in the barley and rice genomes . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: We purified NAS protein and isolated the genes nas1 , nas2 , nas3 , nas4 , nas5-1 , nas5-2 , and nas6 , which encode NAS and NAS-like proteins from Fe-deficient barley ( Hordeum vulgare L cv Ehimehadaka no 1 ) roots .
[ Sen. 3, subscore: 1.00 ]: Escherichia coli expressing nas1 showed NAS activity , confirming that this gene encodes a functional NAS .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa .
| | Author: Wu J Wang C Zheng L Wang L Chen Y Whelan J Shou H | | Journal: J Exp Bot Citation: V : 62 P : 667-74 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21112958 Accession (PMID): 21112958 | | Abstract: Plants employ two distinct strategies to obtain iron ( Fe ) from the soil .
In Strategy I but not Strategy II plants , Fe limitation invokes ethylene production which regulates Fe deficiency responses .
Oryza sativa ( rice ) is the only graminaceous plant described that possesses a Strategy I-like system for iron uptake as well as the classic Strategy II system .
Ethylene production of rice roots was significantly increased when grown under Fe-depleted conditions .
Moreover , 1-aminocyclopropane-1-carboxylic acid ( ACC ) treatment , a precursor of ethylene , conferred tolerance to Fe deficiency in rice by increasing internal Fe availability .
Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2 , nicotianamine synthases 1 and 2 ( NAS1 and NAS2 ) , yellow-stripe like transporter 15 ( YSL15 ) and iron-regulated transporter ( IRT1 ) indicated that ethylene caused an increase in transcript abundance of both Fe ( II ) and Fe ( III ) -phytosiderophore uptake systems .
RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1 , OsNAS2 , OsYSL15 , and OsIRT1 .
By contrast , in Hordeum vulgare L ( barley ) , no ethylene production or ethylene-mediated effects of Fe response could be detected .
In conclusion , Fe-limiting conditions increased ethylene production and signalling in rice , which is novel in Strategy II plant species .
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[ Sen. 6, subscore: 1.00 ]: Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2 , nicotianamine synthases 1 and 2 ( NAS1 and NAS2 ) , yellow-stripe like transporter 15 ( YSL15 ) and iron-regulated transporter ( IRT1 ) indicated that ethylene caused an increase in transcript abundance of both Fe ( II ) and Fe ( III ) -phytosiderophore uptake systems .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Interspecies compatibility of NAS1 gene promoters .
| | Author: Ito S Inoue H Kobayashi T Yoshiba M Mori S Nishizawa N Higuchi K | | Journal: Plant Physiol Biochem Citation: V : 45 P : 270-6 Year: 2007 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub17524656 Accession (PMID): 17524656 | | Abstract: Nicotianamine and nicotianamine synthase ( NAS ) play key roles in iron nutrition in all higher plants .
However , the mechanism underlying the regulation of NAS expression differs among plant species .
Sequences homologous to iron deficiency-responsive elements ( IDEs ) , ie , cis-acting elements , are found on the promoters of these genes .
We aimed to verify the interspecies compatibility of the Fe-deficiency response of NAS1 genes and understand the universal mechanisms that regulate their expression patterns in higher plants .
Therefore , we introduced the graminaceous ( Hordeum vulgare L and Oryza sativa L ) NAS1 promoter : : GUS into dicots ( Nicotiana tabacum L and Arabidopsis thaliana L ) .
Fe deficiency induced HvNAS1 expression in the shoots and roots when introduced into rice .
HvNAS1 promoter : : GUS and OsNAS1 promoter : : GUS induced strong expression of GUS under Fe-deficient conditions in transformed tobacco In contrast , these promoters only definitely functioned in Arabidopsis transformants .
These results suggest that some Fe nutrition-related trans-factors are not compatible between graminaceous plants and Arabidopsis .
HvNAS1 promoter : : GUS induced GUS activity only in the roots of transformed tobacco under Fe-deficient conditions .
On the other hand , OsNAS1 promoter : : GUS induced GUS activity in both the roots and shoots of transformed tobacco under conditions of Fe deficiency .
In tobacco transformants , the induction of GUS activity was induced earlier in the shoots than roots .
These results suggest that the HvNAS1 and OsNAS1 promoters are compatible with Fe-acquisition-related trans-factors in the roots of tobacco and that the OsNAS1 promoter is also compatible with some shoot-specific Fe deficiency-related trans-factors in tobacco | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Interspecies compatibility of NAS1 gene promoters .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
