| 10 matches found in 7 documents. Search time: 0.104 seconds. |
|---|
|
Score: 3.00 |
|---|
| Title: OsMTN encodes a 5-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice ( Oryza sativa L ) .
| | Author: Rzewuski G Cornell KA Rooney L BEstenbinder K Wirtz M Hell R Sauter M | | Journal: Citation: V : ( ) P : Year: 2007 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17339651 Accession (PMID): 17339651 | | Abstract: Methylthioadenosine ( MTA ) is released as a by-product of S-adenosylmethionine ( AdoMet ) -dependent reactions central to ethylene , polyamine , or phytosiderophore biosynthesis .
MTA is hydrolysed by methylthioadenosine nucleosidase ( MTN ; EC 3 . 2 . 2 . 16 ) into adenine and methylthioribose which is processed through the methionine ( Met ) cycle to produce a new molecule of AdoMet .
In deepwater rice , submergence enhances ethylene biosynthesis , and ethylene in turn influences the methionine cycle through positive feedback regulation of the acireductone dioxygenase gene OsARD1 .
In rice , MTN is encoded by a single gene designated OsMTN .
Recombinant OsMTN enzyme had a K ( M ) for MTA of 2 . 1 mM and accepted a wide array of 5 substitutions of the substrate .
OsMTN also metabolized S-adenosylhomocysteine ( AdoHcy ) with 15 . 9% the rate of MTA .
OsMTN transcripts and OsMTN-specific activity increased slowly and in parallel upon submergence , indicating that regulation occurred mainly at the transcriptional level .
Neither ethylene , MTA , nor Met regulated OsMTN expression .
Analysis of steady-state metabolite levels showed that MTN activity was sufficiently high to prevent Met and AdoMet depletion during long-term ethylene biosynthesis .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: MTA is hydrolysed by methylthioadenosine nucleosidase ( MTN ; EC 3 . 2 . 2 . 16 ) into adenine and methylthioribose which is processed through the methionine ( Met ) cycle to produce a new molecule of AdoMet .
[ Sen. 4, subscore: 1.00 ]: In rice , MTN is encoded by a single gene designated OsMTN .
[ Sen. 9, subscore: 1.00 ]: Analysis of steady-state metabolite levels showed that MTN activity was sufficiently high to prevent Met and AdoMet depletion during long-term ethylene biosynthesis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: Unique properties of NADP-thioredoxin reductase C in legumes .
| | Author: Alkhalfioui F Renard M Montrichard F | | Journal: Citation: V : ( ) P : Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17185738 Accession (PMID): 17185738 | | Abstract: NADP-thioredoxin reductases ( NTRs ) reduce thioredoxins ( Trxs ) , using NADPH as a reductant , together constituting complete redox systems ( NTS ) .
Beside NTRA and NTRB targeted to both cytosol and mitochondria of plant cells , there is in chloroplasts an unusual NTR ( NTRC ) harbouring a Trx domain in a C-terminal extension , as recently reported in Oryza sativa .
Although NTRC may constitute a complete NTS , it was described as a bifunctional enzyme .
Because the gene is only present in photosynthetic organisms and the protein in green it issues , NTRC was thought to have a role restricted to photosynthetic cells .
To determine whether NTRC from dicot plants is a bifunctional enzyme or a complete NTS , as well as to identify its putative target , NTRC from Medicago truncatula was cloned and NTRA was cloned for comparison .
Here evidence is presented that MtNTRC ( i ) acts as an NTS and reduces dithiobisnitrobenzoate ( DTNB ) with a turnover ( 0 . 62 s ( -1 ) ) similar to that measured with MtNTRA in the presence of a Trxh ( 0 . 81 s ( -1 ) ) ; ( ii ) is able to use both NADPH ( k ( M ) =2 . 4 muM ) and NADH ( k ( M ) =11 muM ) as cofactors ; ( iii ) efficiently reduces BAS1 , a plastidial peroxiredoxin ; and ( iv ) is expressed in both leaves and stems but unexpectedly is even more abundant in cotyledons from dry and germinating seeds .
Because BAS1 is also present in both green it issues and seeds , NTRC/BAS1 may be involved in the scavenging of peroxides produced in green it issues during the day or the night and in seeds during germination .
These results suggest different roles for NTRC in monocot and dicot plants . | Matching Sentences:
[ Sen. 6, subscore: 2.00 ]: Here evidence is presented that MtNTRC ( i ) acts as an NTS and reduces dithiobisnitrobenzoate ( DTNB ) with a turnover ( 0 . 62 s ( -1 ) ) similar to that measured with MtNTRA in the presence of a Trxh ( 0 . 81 s ( -1 ) ) ; ( ii ) is able to use both NADPH ( k ( M ) =2 . 4 muM ) and NADH ( k ( M ) =11 muM ) as cofactors ; ( iii ) efficiently reduces BAS1 , a plastidial peroxiredoxin ; and ( iv ) is expressed in both leaves and stems but unexpectedly is even more abundant in cotyledons from dry and germinating seeds .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
|---|
| Title: The ColRS system of Xanthomonas oryzae pv . oryzae is required for virulence and growth in iron-limiting conditions .
| | Author: Subramoni S Pandey A Vishnu Priya MR Patel HK Sonti RV | | Journal: Mol Plant Pathol Citation: V : 13 P : 690-703 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub22257308 Accession (PMID): 22257308 | | Abstract: Xanthomonas oryzae pv . oryzae , the causal agent of bacterial blight of rice , produces siderophores only under iron-limiting conditions .
We screened 15 400 mTn5-induced mutants of X oryzae pv . oryzae and isolated 27 mutants that produced siderophores even under iron-replete conditions .
We found that the mTn5 insertions in 25 of these mutants were in or close to six genes .
Mutants with insertions in five of these genes [ colS , XOO1806 ( a conserved hypothetical protein ) , acnB , prpR and prpB ] exhibited a deficiency for growth on iron-limiting medium and a decrease in virulence .
Insertions in a sixth gene , XOO0007 ( a conserved hypothetical protein ) , were found to affect the ability to grow on iron-limiting medium , but did not affect the virulence .
Targeted gene disruptants for colR ( encoding the predicted cognate regulatory protein for ColS ) also exhibited a deficiency for growth on iron-limiting medium and a decrease in virulence . colR and colS mutants were defective in the elicitation of hypersensitive response symptoms on the nonhost tomato .
In addition , colR and colS mutants induced a rice basal defence response , suggesting that they are compromised in the suppression of host innate immunity .
Quantitative reverse transcription-polymerase chain reaction ( RT-PCR ) analysis demonstrated that a functional ColRS system is required for the optimal expression of several genes encoding components of the type 3 secretion system ( T3SS ) of X oryzae pv . oryzae .
Our results demonstrate the role of several novel genes , including colR/colS , in the promotion of growth on iron-limiting medium and the virulence of X oryzae pv . oryzae .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: We screened 15 400 mTn5-induced mutants of X oryzae pv . oryzae and isolated 27 mutants that produced siderophores even under iron-replete conditions .
[ Sen. 3, subscore: 1.00 ]: We found that the mTn5 insertions in 25 of these mutants were in or close to six genes .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: An auxin-inducible gene from loblolly pine ( Pinus taeda L ) is differentially expressed in mature and juvenile-phase shoots and encodes a putative transmembrane protein .
| | Author: Busov VB Johannes E Whetten RW Sederoff RR Spiker SL Lanz-Garcia C Goldfarb B | | Journal: Planta Citation: V : 218 ( 6 ) P : 916-27 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub14722770 Accession (PMID): 14722770 | | Abstract: We have isolated a gene from loblolly pine , 5NG4 , that is highly and specifically induced by auxin in juvenile loblolly pine shoots prior to adventitious root formation , but substantially down-regulated in physiologically mature shoots that are adventitious rooting incompetent .
5NG4 was highly auxin-induced in roots , stems and hypocotyls , organs that can form either lateral or adventitious roots following an auxin treatment , but was not induced to the same level in needles and cotyledons , organs that do not form roots .
The deduced amino acid sequence shows homology to the MtN21 nodulin gene from Medicago truncatula .
The expression pattern of 5NG4 and its homology to a protein from Medicago involved in a root-related process suggest a possible role for this gene in adventitious root formation .
Homology searches also identified similar proteins in Arabidopsis thaliana and Oryza sativa .
High conservation across these evolutionarily distant species suggests essential functions in plant growth and development .
A 38-member family of genes homologous to 5NG4 was identified in the A thaliana genome .
The physiological significance of this redundancy is most likely associated with functional divergence and/or expression specificity of the different family members .
The exact biochemical function of the gene is still unknown , but sequence and structure predictions and 5NG4 : : GFP fusion protein localizations indicate it is a transmembrane protein with a possible transport function . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: The deduced amino acid sequence shows homology to the MtN21 nodulin gene from Medicago truncatula .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Targeting xa13 , a recessive gene for bacterial blight resistance in rice .
| | Author: Chu Z Fu B Yang H Xu C Li Z Sanchez A Park YJ Bennetzen JL Zhang Q Wang S | | Journal: Theor . Appl . Genet . Citation: V : 112 ( 3 ) P : 455-61 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16328230 Accession (PMID): 16328230 | | Abstract: Bacterial blight , caused by Xanthomonas oryzae pv . oryzae ( Xoo ) , is one of the most serious diseases of rice worldwide .
Thirty bacterial blight resistance ( R ) genes ( 21 dominant genes and 9 recessive genes ) in rice have been identified .
They are the main sources for the genetic improvement of rice for resistance to Xoo .
However , little is known about the recessive R genes .
To clone and characterize the recessive R genes , we fine-mapped xa13 , a fully recessive gene for Xoo resistance , to a DNA fragment of 14 . 8 kb using the map-based cloning strategy and a series of sequence-based molecular markers .
Sequence analysis of this fragment indicated that this region contains only two apparently intact candidate genes ( an extensin-like gene and a homologue of nodulin MtN3 ) and the 5 end of a predicted hypothetical gene .
These results will greatly facilitate the isolation and characterization of xa13 .
Four PCR-based markers , E6a , SR6 , ST9 and SR11 that were tightly linked to the xa13 locus , were also developed .
These markers will be useful tools for the marker-assisted selection of xa13 in breeding programs . | Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: Sequence analysis of this fragment indicated that this region contains only two apparently intact candidate genes ( an extensin-like gene and a homologue of nodulin MtN3 ) and the 5 end of a predicted hypothetical gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
