Score: 5.00 |
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| Title: Genetic analysis and molecular mapping of a nuclear recessive male sterility gene , ms91 ( t ) , in rice .
| | Author: Liu X Wang S Wang Y Wei S | | Journal: Genome Citation: V : 50 P : 796-801 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17893719 Accession (PMID): 17893719 | | Abstract: Mutations that result in plant male sterility provide means not only to probe reproductive development but also to facilitate commercial heterosis application and hybrid seed production .
In this study , we report a novel male sterility gene , ms91 ( t ) , in a spontaneous mutant line ( SH38 ) from a Chinese rice cultivar ( Oryza sativa subsp . japonica Jijing14 ) .
The sterility of SH38 was studied by examining its progenies derived from crosses with 6 japonica cultivars .
Corresponding F2 populations were obtained by selfing each of the 6 F1s and a backcross population was produced by crossing SH38 to the F1 of SH38 x C18 .
Our results revealed that SH38 has normal agronomic traits but produces no pollen grains .
Segregations of male-sterile and male-fertile progenies in the F2 and backcross populations fit well with ratios of 3 : 1 and 1 : 1 , respectively , indicating that ms91 ( t ) is a single recessive gene .
Amplified fragment length polymorphism ( AFLP ) analysis of SH38 and Jijing14 plants showed the presence of a unique band in SH38 .
Simple sequence repeat ( SSR ) analysis of the bulked and individual progenies of the F2 population of SH38 x C18 showed linkage of ms91 ( t ) with the SSR marker RM5853 on chromosome 1 .
Subsequently , ms91 ( t ) was fine-mapped to the interval between markers RM7075 ( 3 . 75 cM ) and RM5638 ( 3 . 57 cM ) .
Our results would facilitate the isolation of ms91 ( t ) and male sterility in heterosis application .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In this study , we report a novel male sterility gene , ms91 ( t ) , in a spontaneous mutant line ( SH38 ) from a Chinese rice cultivar ( Oryza sativa subsp . japonica Jijing14 ) .
[ Sen. 6, subscore: 1.00 ]: Segregations of male-sterile and male-fertile progenies in the F2 and backcross populations fit well with ratios of 3 : 1 and 1 : 1 , respectively , indicating that ms91 ( t ) is a single recessive gene .
[ Sen. 8, subscore: 1.00 ]: Simple sequence repeat ( SSR ) analysis of the bulked and individual progenies of the F2 population of SH38 x C18 showed linkage of ms91 ( t ) with the SSR marker RM5853 on chromosome 1 .
[ Sen. 9, subscore: 1.00 ]: Subsequently , ms91 ( t ) was fine-mapped to the interval between markers RM7075 ( 3 . 75 cM ) and RM5638 ( 3 . 57 cM ) .
[ Sen. 10, subscore: 1.00 ]: Our results would facilitate the isolation of ms91 ( t ) and male sterility in heterosis application .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
|---|
| Title: Genetic analysis and molecular mapping of a nuclear recessive male sterility gene , ms91 ( t ) , in rice .
| | Author: Liu X Wang S Wang Y Wei S | | Journal: Genome Citation: V : 50 P : 796-801 Year: 2007 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub17893719 Accession (PMID): 17893719 | | Abstract: Mutations that result in plant male sterility provide means not only to probe reproductive development but also to facilitate commercial heterosis application and hybrid seed production .
In this study , we report a novel male sterility gene , ms91 ( t ) , in a spontaneous mutant line ( SH38 ) from a Chinese rice cultivar ( Oryza sativa subsp . japonica Jijing14 ) .
The sterility of SH38 was studied by examining its progenies derived from crosses with 6 japonica cultivars .
Corresponding F2 populations were obtained by selfing each of the 6 F1s and a backcross population was produced by crossing SH38 to the F1 of SH38 x C18 .
Our results revealed that SH38 has normal agronomic traits but produces no pollen grains .
Segregations of male-sterile and male-fertile progenies in the F2 and backcross populations fit well with ratios of 3 : 1 and 1 : 1 , respectively , indicating that ms91 ( t ) is a single recessive gene .
Amplified fragment length polymorphism ( AFLP ) analysis of SH38 and Jijing14 plants showed the presence of a unique band in SH38 .
Simple sequence repeat ( SSR ) analysis of the bulked and individual progenies of the F2 population of SH38 x C18 showed linkage of ms91 ( t ) with the SSR marker RM5853 on chromosome 1 .
Subsequently , ms91 ( t ) was fine-mapped to the interval between markers RM7075 ( 3 . 75 cM ) and RM5638 ( 3 . 57 cM ) .
Our results would facilitate the isolation of ms91 ( t ) and male sterility in heterosis application .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Genetic analysis and molecular mapping of a nuclear recessive male sterility gene , ms91 ( t ) , in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
