22 matches found in 10 documents. Search time: 0.242 seconds. |
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Score: 10.00 | Title: Improving the reproductive performance of Egyptian buffalo cows by changing the management system .
| Author: Abdalla EB .
| Journal: Anim . Reprod . Sci . Citation: V : 75 ( 1-2 ) P : 1-8 Year: 2003 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12535580 Accession (PMID): 12535580 | Abstract: The objective of the present study was to determine the possibility to improve the reproductive performance of buffalo cows through the continuous exposure to bull with grazing and free-stall housing management .
Sixty-four Egyptian multiparous buffalo cows raised under two different management systems in two farms were used in this study .
The cows in the first farm ( management system 1 , MS1 ) were loose--housed in a free-stall yard , grazed for 4 h per day , suckled their calves for 2-3 months and were continuously exposed to a fertile bull .
The cows in the second farm ( management system 2 , MS2 ) were confined in an open-fronted tie-stall shed , not grazed , suckled their calves for only 7 days and were exposed to a fertile bull twice per day ( 30 min per session ) .
All the cows were fed a diet of green berseem ( Trifolium alexandrinum ) , rice straw and concentrates to meet their maintenance and production requirements .
The cows during both the treatments were milked twice per day after weaning .
The cows in both groups were between the second and the sixth parity , weighed 450-480 kg and had average daily milk yields of 5 . 0-6 . 0 kg .
In each farm , cows were visually checked twice daily at 07 : 00 and 17 : 00 h for the signs of oestrus and animals proved standing heat were naturally mated .
Rectal palpation was used to monitor uterine involution and for pregnancy diagnosis .
Blood was sampled twice per week from 7 to 150 days post-partum for serum progesterone assay .
The results revealed that post-partum intervals to each of first ovulation , first oestrus , conception and next parturition were significantly ( P < 0 . 05 ) shorter in MS1 group than in MS2 group .
In the meantime , MS1 increased ( P < 0 . 01 ) the conception and calving rates by 21 and 25% , respectively compared to MS2 .
Percentages of post-partum cyclic animals and animals exhibiting ovulatory oestrus were greater ( P < 0 . 01 ) in MS1 group than in MS2 group .
However , the percentage of animals cycling before day 60 post-partum was significantly ( P < 0 . 01 ) lower in MS1 group than in MS2 group ( 13% versus 28% ) .
By day 120 post-partum , only 63% of the buffaloes were cycling in MS2 group versus 94% in MS1 group .
Percentage of silent ovulation was insignificantly higher in MS2 group ( 34% ) than in MS1 group ( 25% ) .
However , the percentage of false oestrus was higher ( P < 0 . 01 ) in MS1 group than in MS2 group ( 16% versus 3% ) .
In addition , percentage of short ovulatory cycles ( 15-17 days ) was greater ( P < 0 . 01 ) in MS1 group than in MS2 group , whereas percentage of long ovulatory cycle ( 25-28 days ) was higher ( P < 0 . 01 ) in MS2 group than in MS1 group .
It was concluded that continuous exposure of buffalo cows to a fertile bull with grazing management under free-stall housing system enhances resumption of post-partum ovarian activity and improves conception and calving rates .
| Matching Sentences: [ Sen. 18, subscore: 2.00 ]: In addition , percentage of short ovulatory cycles ( 15-17 days ) was greater ( P < 0 . 01 ) in MS1 group than in MS2 group , whereas percentage of long ovulatory cycle ( 25-28 days ) was higher ( P < 0 . 01 ) in MS2 group than in MS1 group . [ Sen. 4, subscore: 1.00 ]: The cows in the second farm ( management system 2 , MS2 ) were confined in an open-fronted tie-stall shed , not grazed , suckled their calves for only 7 days and were exposed to a fertile bull twice per day ( 30 min per session ) . [ Sen. 11, subscore: 1.00 ]: The results revealed that post-partum intervals to each of first ovulation , first oestrus , conception and next parturition were significantly ( P < 0 . 05 ) shorter in MS1 group than in MS2 group . [ Sen. 12, subscore: 1.00 ]: In the meantime , MS1 increased ( P < 0 . 01 ) the conception and calving rates by 21 and 25% , respectively compared to MS2 . [ Sen. 13, subscore: 1.00 ]: Percentages of post-partum cyclic animals and animals exhibiting ovulatory oestrus were greater ( P < 0 . 01 ) in MS1 group than in MS2 group . [ Sen. 14, subscore: 1.00 ]: However , the percentage of animals cycling before day 60 post-partum was significantly ( P < 0 . 01 ) lower in MS1 group than in MS2 group ( 13% versus 28% ) . [ Sen. 15, subscore: 1.00 ]: By day 120 post-partum , only 63% of the buffaloes were cycling in MS2 group versus 94% in MS1 group . [ Sen. 16, subscore: 1.00 ]: Percentage of silent ovulation was insignificantly higher in MS2 group ( 34% ) than in MS1 group ( 25% ) . [ Sen. 17, subscore: 1.00 ]: However , the percentage of false oestrus was higher ( P < 0 . 01 ) in MS1 group than in MS2 group ( 16% versus 3% ) .
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Score: 5.00 | Title: Suppression subtractive hybridization analysis of Ms2 near-isogenic lines of wheat reveals genes differentially expressed in spikelets and anthers .
| Author: Zhang ZZ Ma ZQ Liu DJ .
| Journal: Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao Citation: V : 31 ( 2 ) P : 175-82 Year: 2005 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub15840936 Accession (PMID): 15840936 | Abstract: The dominant male sterility gene Ms2 in wheat has been widely used in recurrent selection and variety improvement .
Identification of genes associated with the male sterility in Ms2-carrying wheat will help us understand how Ms2 functions .
Using a pair of isogenic lines of Ms2 , subtractive hybridization was conducted with cDNA from bulked spikelets at meiophase of sterile plants as the tester and cDNA from the same it issues of fertile plants as the driver .
Two major bands at 270 bp and 450 bp were obtained by suppression PCR ( polymerase chain reaction ) of the subtractive cDNA .
A total of 882 recombinants from PCR product cloning were isolated for reverse Northern analysis .
The results demonstrated that up to 90% of the inserts in the library were up-regulated in the sterile spikelets .
Twenty-one unique inserts from this library were sequenced .
Similarity search showed that eighteen of them were homologous to ESTs ( expression sequence tags ) derived from spike or anther it issues at meiophase .
The chromosome locations of nine of the ESTs were determined using CS ( Chinese spring ) nulli-tetrasomic lines , one of which was assigned to chromosome group 4 that includes chromosome 4D where Ms2 is located .
In addition , four additional ESTs could also be assigned to this group according to their homology to BACs ( bacterial artificial chromosomes ) or PAC ( P1 artificial chromosomes ) of rice chromosome 3 .
The expression patterns of eight of the inserts examined displayed increased expression in spikelets and anthers of the sterile plants .
| Matching Sentences: [ Sen. 2, subscore: 2.00 ]: Identification of genes associated with the male sterility in Ms2-carrying wheat will help us understand how Ms2 functions . [ Sen. 1, subscore: 1.00 ]: The dominant male sterility gene Ms2 in wheat has been widely used in recurrent selection and variety improvement . [ Sen. 3, subscore: 1.00 ]: Using a pair of isogenic lines of Ms2 , subtractive hybridization was conducted with cDNA from bulked spikelets at meiophase of sterile plants as the tester and cDNA from the same it issues of fertile plants as the driver . [ Sen. 9, subscore: 1.00 ]: The chromosome locations of nine of the ESTs were determined using CS ( Chinese spring ) nulli-tetrasomic lines , one of which was assigned to chromosome group 4 that includes chromosome 4D where Ms2 is located .
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Score: 2.00 | Title: The transport of prolamine RNAs to prolamine protein bodies in living rice endosperm cells .
| Author: Hamada S Ishiyama K Choi SB Wang C Singh S Kawai N Franceschi VR Okita TW .
| Journal: Plant Cell Citation: V : 15 ( 10 ) P : 2253-64 Year: 2003 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub14508010 Accession (PMID): 14508010 | Abstract: RNAs that code for the major rice storage proteins are localized to specific subdomains of the cortical endoplasmic reticulum ( ER ) in developing endosperm .
Prolamine RNAs are localized to the ER and delimit the prolamine intracisternal inclusion granules ( PB-ER ) , whereas glutelin RNAs are targeted to the cisternal ER .
To study the transport of prolamine RNAs to the surface of the prolamine protein bodies in living endosperm cells , we adapted a two-gene system consisting of green fluorescent protein ( GFP ) fused to the viral RNA binding protein MS2 and a hybrid prolamine RNA containing tandem MS2 RNA binding sites .
Using laser scanning confocal microscopy , we show that the GFP-labeled prolamine RNAs are transported as particles that move at an average speed of 0 . 3 to 0 . 4 microm/s .
These prolamine RNA transport particles generally move unidirectionally in a stop-and-go manner , although nonlinear bidirectional , restricted , and nearly random movement patterns also were observed .
Transport is dependent on intact microfilaments , because particle movement is inhibited rapidly by the actin filament-disrupting drugs cytochalasin D and latrunculin B Direct evidence was obtained that these prolamine RNA-containing particles are transported to the prolamine protein bodies .
The significance of these results with regard to protein synthesis in plants is discussed . | Matching Sentences: [ Sen. 3, subscore: 2.00 ]: To study the transport of prolamine RNAs to the surface of the prolamine protein bodies in living endosperm cells , we adapted a two-gene system consisting of green fluorescent protein ( GFP ) fused to the viral RNA binding protein MS2 and a hybrid prolamine RNA containing tandem MS2 RNA binding sites .
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Score: 2.00 | Title: Defective pollen wall is required for anther and microspore development in rice and encodes a Fatty acyl carrier protein reductase .
| Author: Shi J Tan H Yu XH Liu Y Liang W Ranathunge K Franke RB Schreiber L Wang Y Kai G Shanklin J Ma H Zhang D | Journal: Plant Cell Citation: V : 23 P : 2225-46 Year: 2011 Type: In-Process | Literature: oryza Field: abstract Doc ID: pub21705642 Accession (PMID): 21705642 | Abstract: Aliphatic alcohols naturally exist in many organisms as important cellular components ; however , their roles in extracellular polymer biosynthesis are poorly defined .
We report here the isolation and characterization of a rice ( Oryza sativa ) male-sterile mutant , defective pollen wall ( dpw ) , which displays defective anther development and degenerated pollen grains with an irregular exine .
Chemical analysis revealed that dpw anthers had a dramatic reduction in cutin monomers and an altered composition of cuticular wax , as well as soluble fatty acids and alcohols .
Using map-based cloning , we identified the DPW gene , which is expressed in both tapetal cells and microspores during anther development .
Biochemical analysis of the recombinant DPW enzyme shows that it is a novel fatty acid reductase that produces 1-hexadecanol and exhibits >270-fold higher specificity for palmiltoyl-acyl carrier protein than for C16 : 0 CoA substrates .
DPW was predominantly targeted to plastids mediated by its N-terminal transit peptide .
Moreover , we demonstrate that the monocot DPW from rice complements the dicot Arabidopsis thaliana male sterile2 ( ms2 ) mutant and is the probable ortholog of MS2 .
These data suggest that DPWs participate in a conserved step in primary fatty alcohol synthesis for anther cuticle and pollen sporopollenin biosynthesis in monocots and dicots .
| Matching Sentences: [ Sen. 7, subscore: 2.00 ]: Moreover , we demonstrate that the monocot DPW from rice complements the dicot Arabidopsis thaliana male sterile2 ( ms2 ) mutant and is the probable ortholog of MS2 .
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Score: 2.00 | Title: Alumoxane/ferroxane nanoparticles for the removal of viral pathogens : the importance of surface functionality to nanoparticle activity .
| Author: Maguire-Boyle SJ Liga MV Li Q Barron AR | Journal: Nanoscale Citation: V : P : Year: 2012 Type: Publisher | Literature: oryza Field: abstract Doc ID: pub22864822 Accession (PMID): 22864822 | Abstract: A bi-functional nano-composite coating has been created on a porous Nomex ( R ) fabric support as a trap for aspirated virus contaminated water .
Nomex ( R ) fabric was successively dip-coated in solutions containing cysteic acid functionalized alumina ( alumoxane ) nanoparticles and cysteic acid functionalized iron oxide ( ferroxane ) nanoparticles to form a nanoparticle coated Nomex ( R ) ( NPN ) fabric .
From SEM and EDX the nanoparticle coating of the Nomex ( R ) fibers is uniform , continuous , and conformal The NPN was used as a filter for aspirated bacteriophage MS2 viruses using end-on filtration .
All measurements were repeated to give statistical reliability .
The NPN fabrics show a large decrease as compared to Nomex ( R ) alone or alumoxane coated Nomex ( R ) .
An increase in the ferroxane content results in an equivalent increase in virus retention .
This suggests that it is the ferroxane that has an active role in deactivating and/or binding the virus .
Heating the NPN to 160 degrees C results in the loss of cysteic acid functional groups ( without loss of the iron nanoparticles core structure ) and the resulting fabric behaves similar to that of untreated Nomex ( R ) , showing that the surface functionalization of the nanoparticles is vital for the surface collapse of aspirated water droplets and the absorption and immobilization of the MS2 viruses .
Thus , for virus immobilization , it is not sufficient to have iron oxide nanoparticles per se , but the surface functionality of a nanoparticle is vitally important in ensuring efficacy .
| Matching Sentences: [ Sen. 3, subscore: 1.00 ]: From SEM and EDX the nanoparticle coating of the Nomex ( R ) fibers is uniform , continuous , and conformal The NPN was used as a filter for aspirated bacteriophage MS2 viruses using end-on filtration . [ Sen. 8, subscore: 1.00 ]: Heating the NPN to 160 degrees C results in the loss of cysteic acid functional groups ( without loss of the iron nanoparticles core structure ) and the resulting fabric behaves similar to that of untreated Nomex ( R ) , showing that the surface functionalization of the nanoparticles is vital for the surface collapse of aspirated water droplets and the absorption and immobilization of the MS2 viruses .
| Supplemental links/files: reference in endnote online text related articles pubmed citation | |