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3 matches found in 2 documents. Search time: 0.285 seconds.
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Score: 2.00
Title: Homologous recombination-mediated knock-in targeting of the MET1a gene for a maintenance DNA methyltransferase reproducibly reveals dosage-dependent spatiotemporal gene expression in rice .
Author: Yamauchi T Johzuka-Hisatomi Y Fukada-Tanaka S Terada R Nakamura I Iida S
Journal: Plant J Citation: V : 60 P : 386-96 Year: 2009 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub19519802 Accession (PMID): 19519802
Abstract: Although homologous recombination-promoted knock-in targeting to monitor the expression of a gene by fusing a reporter gene with its promoter is routine practice in mice , gene targeting to modify endogenous genes in flowering plants remains in its infancy . In the knock-in targeting , the junction sequence between a reporter gene and an endogenous target promoter can be designed properly , and transgenic plants carrying an identical and desired knock-in allele can be repeatedly obtained . By employing a reproducible gene-targeting procedure with positive-negative selection in rice , we were able to obtain fertile transgenic knock-in plants with the promoterless GUS reporter gene encoding beta-glucuronidase fused with the endogenous promoter of MET1a , one of two rice MET1 genes encoding a maintenance DNA methyltransferase . All of the primary ( T ( 0 ) ) transgenic knock-in plants obtained were found to carry only one copy of GUS , with the anticipated structure in the heterozygous condition , and no ectopic events associated with gene targeting could be detected . We showed the reproducible , dosage-dependent and spatiotemporal expression of GUS in the selfed progenies of independently isolated knock-in targeted plants . The results in knock-in targeted plants contrast sharply with the results in transgenic plants with the MET1a promoter-fused GUS reporter gene integrated randomly in the genome : clear interindividual variation of GUS expression was observed among independently obtained plants bearing the randomly integrated transgenes . As our homologous recombination-mediated gene-targeting strategy with positive-negative selection is , in principle , applicable to modify any endogenous gene , knock-in targeting would facilitate basic and applied plant research .
Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: By employing a reproducible gene-targeting procedure with positive-negative selection in rice , we were able to obtain fertile transgenic knock-in plants with the promoterless GUS reporter gene encoding beta-glucuronidase fused with the endogenous promoter of MET1a , one of two rice MET1 genes encoding a maintenance DNA methyltransferase .
[ Sen. 6, subscore: 1.00 ]: The results in knock-in targeted plants contrast sharply with the results in transgenic plants with the MET1a promoter-fused GUS reporter gene integrated randomly in the genome : clear interindividual variation of GUS expression was observed among independently obtained plants bearing the randomly integrated transgenes .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Homologous recombination-mediated knock-in targeting of the MET1a gene for a maintenance DNA methyltransferase reproducibly reveals dosage-dependent spatiotemporal gene expression in rice .
Author: Yamauchi T Johzuka-Hisatomi Y Fukada-Tanaka S Terada R Nakamura I Iida S
Journal: Plant J Citation: V : 60 P : 386-96 Year: 2009 Type: MEDLINE
Literature: oryza Field: title Doc ID: pub19519802 Accession (PMID): 19519802
Abstract: Although homologous recombination-promoted knock-in targeting to monitor the expression of a gene by fusing a reporter gene with its promoter is routine practice in mice , gene targeting to modify endogenous genes in flowering plants remains in its infancy . In the knock-in targeting , the junction sequence between a reporter gene and an endogenous target promoter can be designed properly , and transgenic plants carrying an identical and desired knock-in allele can be repeatedly obtained . By employing a reproducible gene-targeting procedure with positive-negative selection in rice , we were able to obtain fertile transgenic knock-in plants with the promoterless GUS reporter gene encoding beta-glucuronidase fused with the endogenous promoter of MET1a , one of two rice MET1 genes encoding a maintenance DNA methyltransferase . All of the primary ( T ( 0 ) ) transgenic knock-in plants obtained were found to carry only one copy of GUS , with the anticipated structure in the heterozygous condition , and no ectopic events associated with gene targeting could be detected . We showed the reproducible , dosage-dependent and spatiotemporal expression of GUS in the selfed progenies of independently isolated knock-in targeted plants . The results in knock-in targeted plants contrast sharply with the results in transgenic plants with the MET1a promoter-fused GUS reporter gene integrated randomly in the genome : clear interindividual variation of GUS expression was observed among independently obtained plants bearing the randomly integrated transgenes . As our homologous recombination-mediated gene-targeting strategy with positive-negative selection is , in principle , applicable to modify any endogenous gene , knock-in targeting would facilitate basic and applied plant research .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Homologous recombination-mediated knock-in targeting of the MET1a gene for a maintenance DNA methyltransferase reproducibly reveals dosage-dependent spatiotemporal gene expression in rice .
Supplemental links/files: reference in endnote online text related articles pubmed citation
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