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| Title: Identification and characterization of a rice MCM2 homologue required for DNA replication .
| | Author: Cho JH Kim HB Kim HS Choi SB | | Journal: BMB Rep Citation: V : 41 P : 581-6 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18755073 Accession (PMID): 18755073 | | Abstract: The pre-replication complex ( pre-RC ) , including the core hexameric MCM2-7 complex , ensures that the eukaryotic genome is replicated only once per cell division cycle .
In this study , we identified a rice minichromosome maintenance ( MCM ) homologue ( OsMCM2 ) that functionally complemented fission yeast MCM2 ( CDC19 ) mutants .
We found OsMCM2 transcript expression in roots , leaves , and seeds , although expression levels differed slightly among the organs .
Likewise , the OsMCM2 protein was ubiquitously expressed , but it was downregulated when nutritients were limiting , indicating that MCM2 expression ( and therefore cell cycle progression ) requires adequate nutrition .
Yeast two-hybrid and GST pull-down assays demonstrated that OsMCM2 interacted with the COP9 signalosome 5 ( CSN5 ) .
Taken as a whole , our results indicated that OsMCM2 functions as a subunit of the rice MCM complex and interacts with CSN5 during developmental regulation .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The pre-replication complex ( pre-RC ) , including the core hexameric MCM2-7 complex , ensures that the eukaryotic genome is replicated only once per cell division cycle .
[ Sen. 2, subscore: 1.00 ]: In this study , we identified a rice minichromosome maintenance ( MCM ) homologue ( OsMCM2 ) that functionally complemented fission yeast MCM2 ( CDC19 ) mutants .
[ Sen. 4, subscore: 1.00 ]: Likewise , the OsMCM2 protein was ubiquitously expressed , but it was downregulated when nutritients were limiting , indicating that MCM2 expression ( and therefore cell cycle progression ) requires adequate nutrition .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Plant MCM proteins : role in DNA replication and beyond .
| | Author: Tuteja N Tran NQ Dang HQ Tuteja R | | Journal: Plant Mol Biol Citation: V : 77 P : 537-45 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub22038093 Accession (PMID): 22038093 | | Abstract: Mini-chromosome maintenance ( MCM ) proteins form heterohexameric complex ( MCM2-7 ) to serve as licensing factor for DNA replication to make sure that genomic DNA is replicated completely and accurately once during S phase in a single cell cycle .
MCMs were initially identified in yeast for their role in plasmid replication or cell cycle progression .
Each of six MCM contains highly conserved sequence called "MCM box" , which contains two ATPase consensus Walker A and Walker B motifs .
Studies on MCM proteins showed that ( a ) the replication origins are licensed by stable binding of MCM2-7 to form pre-RC ( pre-replicative complex ) during G1 phase of the cell cycle , ( b ) the activation of MCM proteins by CDKs ( cyclin-dependent kinases ) and DDKs ( Dbf4-dependent kinases ) and their helicase activity are important for pre-RC to initiate the DNA replication , and ( c ) the release of MCMs from chromatin renders the origins "unlicensed" .
DNA replication licensing in plant is , in general , less characterized .
The MCMs have been reported from Arabidopsis , maize , tobacco , pea and rice , where they are found to be highly expressed in dividing it issues such as shoot apex and root tips , localized in nucleus and cytosol and play important role in DNA replication , megagametophyte and embryo development .
The identification of six MCM coding genes from pea and Arabidopsis suggest six distinct classes of MCM protein in higher plant , and the conserved function right across the eukaryotes .
This overview of MCMs contains an emphasis on MCMs from plants and the novel role of MCM6 in abiotic stress tolerance .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Mini-chromosome maintenance ( MCM ) proteins form heterohexameric complex ( MCM2-7 ) to serve as licensing factor for DNA replication to make sure that genomic DNA is replicated completely and accurately once during S phase in a single cell cycle .
[ Sen. 4, subscore: 1.00 ]: Studies on MCM proteins showed that ( a ) the replication origins are licensed by stable binding of MCM2-7 to form pre-RC ( pre-replicative complex ) during G1 phase of the cell cycle , ( b ) the activation of MCM proteins by CDKs ( cyclin-dependent kinases ) and DDKs ( Dbf4-dependent kinases ) and their helicase activity are important for pre-RC to initiate the DNA replication , and ( c ) the release of MCMs from chromatin renders the origins "unlicensed" .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Identification and characterization of a rice MCM2 homologue required for DNA replication .
| | Author: Cho JH Kim HB Kim HS Choi SB | | Journal: BMB Rep Citation: V : 41 P : 581-6 Year: 2008 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub18755073 Accession (PMID): 18755073 | | Abstract: The pre-replication complex ( pre-RC ) , including the core hexameric MCM2-7 complex , ensures that the eukaryotic genome is replicated only once per cell division cycle .
In this study , we identified a rice minichromosome maintenance ( MCM ) homologue ( OsMCM2 ) that functionally complemented fission yeast MCM2 ( CDC19 ) mutants .
We found OsMCM2 transcript expression in roots , leaves , and seeds , although expression levels differed slightly among the organs .
Likewise , the OsMCM2 protein was ubiquitously expressed , but it was downregulated when nutritients were limiting , indicating that MCM2 expression ( and therefore cell cycle progression ) requires adequate nutrition .
Yeast two-hybrid and GST pull-down assays demonstrated that OsMCM2 interacted with the COP9 signalosome 5 ( CSN5 ) .
Taken as a whole , our results indicated that OsMCM2 functions as a subunit of the rice MCM complex and interacts with CSN5 during developmental regulation .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Identification and characterization of a rice MCM2 homologue required for DNA replication .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
