Score: 11.00 |
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| Title: LEAF TIP NECROSIS1 plays a pivotal role in the regulation of multiple phosphate starvation responses in rice .
| | Author: Hu B Zhu C Li F Tang J Wang Y Lin A Liu L Che R Chu C | | Journal: Plant Physiol Citation: V : 156 P : 1101-15 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21317339 Accession (PMID): 21317339 | | Abstract: Although phosphate ( Pi ) starvation signaling is well studied in Arabidopsis ( Arabidopsis thaliana ) , it is still largely unknown in rice ( Oryza sativa ) .
In this work , a rice leaf tip necrosis1 ( ltn1 ) mutant was identified and characterized .
Map-based cloning identified LTN1 as LOC_Os05g48390 , the putative ortholog of Arabidopsis PHO2 , which plays important roles in Pi starvation signaling .
Analysis of transgenic plants harboring a LTN1 promoter : : beta-glucuronidase construct revealed that LTN1 was preferentially expressed in vascular it issues .
The ltn1 mutant exhibited increased Pi uptake and translocation , which led to Pi overaccumulation in shoots .
In association with enhanced Pi uptake and transport , some Pi transporters were up-regulated in the ltn1 mutant in the presence of sufficient Pi .
Furthermore , the elongation of primary and adventitious roots was enhanced in the ltn1 mutant under Pi starvation , suggesting that LTN1 is involved in Pi-dependent root architecture alteration .
Under Pi-sufficient conditions , typical Pi starvation responses such as stimulation of phosphatase and RNase activities , lipid composition alteration , nitrogen assimilation repression , and increased metal uptake were also activated in ltn1 .
Moreover , analysis of OsmiR399-overexpressing plants showed that LTN1 was down-regulated by OsmiR399 .
Our results strongly indicate that LTN1 is a crucial Pi starvation signaling component downstream of miR399 involved in the regulation of multiple Pi starvation responses in rice .
| Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Analysis of transgenic plants harboring a LTN1 promoter : : beta-glucuronidase construct revealed that LTN1 was preferentially expressed in vascular it issues .
[ Sen. 7, subscore: 2.00 ]: Furthermore , the elongation of primary and adventitious roots was enhanced in the ltn1 mutant under Pi starvation , suggesting that LTN1 is involved in Pi-dependent root architecture alteration .
[ Sen. 2, subscore: 1.00 ]: In this work , a rice leaf tip necrosis1 ( ltn1 ) mutant was identified and characterized .
[ Sen. 3, subscore: 1.00 ]: Map-based cloning identified LTN1 as LOC_Os05g48390 , the putative ortholog of Arabidopsis PHO2 , which plays important roles in Pi starvation signaling .
[ Sen. 5, subscore: 1.00 ]: The ltn1 mutant exhibited increased Pi uptake and translocation , which led to Pi overaccumulation in shoots .
[ Sen. 6, subscore: 1.00 ]: In association with enhanced Pi uptake and transport , some Pi transporters were up-regulated in the ltn1 mutant in the presence of sufficient Pi .
[ Sen. 8, subscore: 1.00 ]: Under Pi-sufficient conditions , typical Pi starvation responses such as stimulation of phosphatase and RNase activities , lipid composition alteration , nitrogen assimilation repression , and increased metal uptake were also activated in ltn1 .
[ Sen. 9, subscore: 1.00 ]: Moreover , analysis of OsmiR399-overexpressing plants showed that LTN1 was down-regulated by OsmiR399 .
[ Sen. 10, subscore: 1.00 ]: Our results strongly indicate that LTN1 is a crucial Pi starvation signaling component downstream of miR399 involved in the regulation of multiple Pi starvation responses in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34 /Yr18 : a disease resistance locus effective against multiple pathogens in wheat .
| | Author: Spielmeyer W Singh RP McFadden H Wellings CR Huerta-Espino J Kong X Appels R Lagudah ES | | Journal: Theor Appl Genet Citation: V : 116 P : 481-90 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18074114 Accession (PMID): 18074114 | | Abstract: The Lr34/Yr18 locus has contributed to durable , non-race specific resistance against leaf rust ( Puccinia triticina ) and stripe rust ( P striiformis f . sp . tritici ) in wheat ( Triticum aestivum ) .
Lr34/Yr18 also cosegregates with resistance to powdery mildew ( Pm38 ) and a leaf tip necrosis phenotype ( Ltn1 ) .
Using a high resolution mapping family from a cross between near-isogenic lines in the "Thatcher" background we demonstrated that Lr34/Yr18 also cosegregated with stem rust resistance in the field .
Lr34/Yr18 probably interacts with unlinked genes to provide enhanced stem rust resistance in "Thatcher" .
In view of the relatively low levels of DNA polymorphism reported in the Lr34/Yr18 region , gamma irradiation of the single chromosome substitution line , Lalbahadur ( Parula7D ) that carries Lr34/Yr18 was used to generate several mutant lines .
Characterisation of the mutants revealed a range of highly informative genotypes , which included variable size deletions and an overlapping set of interstitial deletions .
The mutants enabled a large number of wheat EST derived markers to be mapped and define a relatively small physical region on chromosome 7DS that carried Lr34/Yr18 .
Fine scale genetic mapping confirmed the physical mapping and identified a genetic interval of less than 0 . 5 cM , which contained Lr34/Yr18 .
Both rice and Brachypodium genome sequences provided useful information for fine mapping of ESTs in wheat .
Gene order was more conserved between wheat and Brachypodium than with rice but these smaller grass genomes did not reveal sequence information that could be used to identify a candidate gene for rust resistance in wheat .
We predict that Lr34/Yr18 is located within a large insertion in wheat not found at syntenic positions in Brachypodium and rice .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Lr34/Yr18 also cosegregates with resistance to powdery mildew ( Pm38 ) and a leaf tip necrosis phenotype ( Ltn1 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
