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6 matches found in 3 documents. Search time: 0.061 seconds.
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Score: 3.00
Title: Functional analysis of six drought-inducible promoters in transgenic rice plants throughout all stages of plant growth .
Author: Yi N Kim YS Jeong MH Oh SJ Jeong JS Park SH Jung H Choi YD Kim JK
Journal: Planta Citation: V : 232 P : 743-54 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20567981 Accession (PMID): 20567981
Abstract: There are few efficient promoters for use with stress-inducible gene expression in plants , and in particular for monocotyledonous crops . Here , we report the identification of six genes , Rab21 , Wsi18 , Lea3 , Uge1 , Dip1 , and R1G1B that were induced by drought stress in rice microarray experiments . Gene promoters were linked to the gfp reporter and their activities were analyzed in transgenic rice plants throughout all stages of plant growth , from dry seeds to vegetative it issues to flowers , both before and after drought treatments . In fold induction levels , Rab21 and Wsi18 promoters ranged from 65 and 36-fold in leaves to 1 , 355 and 492-fold in flowers , respectively , whereas Lea3 and Uge1 were higher in leaves , but lower in roots and flowers , as compared with Rab21 and Wsi18 . Dip1 and R1G1B promoters had higher basal levels of activity under normal growth conditions in all it issues , resulting in smaller fold-induction levels than those of the others . In drought treatment time course , activities of Dip1 and R1G1B promoters rapidly increased , peaked at 2 h , and remained constant until 8 h , while that of Lea3 slowly yet steadily increased until 8 h . Interestingly , Rab21 activity increased rapidly and steadily in response to drought stress until expression peaked at 8 h . Thus , we have isolated and characterized six rice promoters that are all distinct in fold induction , it issue specificity , and induction kinetics under drought conditions , providing a variety of drought-inducible promoters for crop biotechnology .
Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Here , we report the identification of six genes , Rab21 , Wsi18 , Lea3 , Uge1 , Dip1 , and R1G1B that were induced by drought stress in rice microarray experiments .
[ Sen. 4, subscore: 1.00 ]: In fold induction levels , Rab21 and Wsi18 promoters ranged from 65 and 36-fold in leaves to 1 , 355 and 492-fold in flowers , respectively , whereas Lea3 and Uge1 were higher in leaves , but lower in roots and flowers , as compared with Rab21 and Wsi18 .
[ Sen. 6, subscore: 1.00 ]: In drought treatment time course , activities of Dip1 and R1G1B promoters rapidly increased , peaked at 2 h , and remained constant until 8 h , while that of Lea3 slowly yet steadily increased until 8 h .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 2.00
Title: Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses ( Poaceae ) .
Author: Yu L Wu X Tang X Yan B
Journal: Genet Mol Res Citation: V : 9 P : 1321-5 Year: 2010 Type: MEDLINE
Literature: oryza Field: abstract Doc ID: pub20623457 Accession (PMID): 20623457
Abstract: cDNA fragments of lea3 genes with a high GC content ( from 68 to 77% ) were found in several Poaceae , including Sorghum vulgare , Saccharum officinarum , Oryza officinalis , Oryza meyeriana , Ampelocalamus calcareus , Cynodon dactylon , and Zizania latifoli . They were successfully isolated by means of optimal experimental parameters , which included dimethyl sulfoxide as additive and degenerate primers "AGETKAS" and "AGKDKTG" , and their sequences were analyzed . Compared to the method of isolating genes by screening of a cDNA library using abscisic acid and other stress-responsive cDNA clones , which is time-consuming and costly , this method is relatively easy and inexpensive . Using this new method , many new homologue lea3 genes were rapidly determined .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: cDNA fragments of lea3 genes with a high GC content ( from 68 to 77% ) were found in several Poaceae , including Sorghum vulgare , Saccharum officinarum , Oryza officinalis , Oryza meyeriana , Ampelocalamus calcareus , Cynodon dactylon , and Zizania latifoli .
[ Sen. 4, subscore: 1.00 ]: Using this new method , many new homologue lea3 genes were rapidly determined .
Supplemental links/files: reference in endnote online text related articles pubmed citation
Score: 1.00
Title: Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses ( Poaceae ) .
Author: Yu L Wu X Tang X Yan B
Journal: Genet Mol Res Citation: V : 9 P : 1321-5 Year: 2010 Type: MEDLINE
Literature: oryza Field: title Doc ID: pub20623457 Accession (PMID): 20623457
Abstract: cDNA fragments of lea3 genes with a high GC content ( from 68 to 77% ) were found in several Poaceae , including Sorghum vulgare , Saccharum officinarum , Oryza officinalis , Oryza meyeriana , Ampelocalamus calcareus , Cynodon dactylon , and Zizania latifoli . They were successfully isolated by means of optimal experimental parameters , which included dimethyl sulfoxide as additive and degenerate primers "AGETKAS" and "AGKDKTG" , and their sequences were analyzed . Compared to the method of isolating genes by screening of a cDNA library using abscisic acid and other stress-responsive cDNA clones , which is time-consuming and costly , this method is relatively easy and inexpensive . Using this new method , many new homologue lea3 genes were rapidly determined .
Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Simple and efficient method for isolating cDNA fragments of lea3 genes with potential for wide application in the grasses ( Poaceae ) .
Supplemental links/files: reference in endnote online text related articles pubmed citation
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