| 9 matches found in 5 documents. Search time: 0.459 seconds. |
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| Title: A novel iron-regulated metal transporter from plants identified by functional expression in yeast | | Author: Eide D Broderius M Fett J Guerinot ML .
| | Journal: Proc . Natl . Acad . Sci . USA Citation: V : 93 ( 11 ) P : 5624-8 Year: 1996 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub8643627 Accession (PMID): 8643627 | | Abstract: Iron is an essential nutrient for virtually all organisms .
The IRT1 ( iron-regulated transporter ) gene of the plant Arabidopsis thaliana , encoding a probable Fe ( II ) transporter , was cloned by functional expression in a yeast strain defective for iron uptake .
Yeast expressing IRT1 possess a novel Fe ( II ) uptake activity that is strongly inhibited by Cd .
IRT1 is predicted to be an integral membrane protein with a metal-binding domain .
Data base comparisons and Southern blot analysis indicated that IRT1 is a member of a gene family in Arabidopsis .
Related sequences were also found in the genomes of rice , yeast , nematodes , and humans .
In Arabidopsis , IRT1 is expressed in roots , is induced by iron deficiency , and has altered regulation in plant lines bearing mutations that affect the iron uptake system .
These results provide the first molecular insight into iron transport by plants . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: The IRT1 ( iron-regulated transporter ) gene of the plant Arabidopsis thaliana , encoding a probable Fe ( II ) transporter , was cloned by functional expression in a yeast strain defective for iron uptake .
[ Sen. 3, subscore: 1.00 ]: Yeast expressing IRT1 possess a novel Fe ( II ) uptake activity that is strongly inhibited by Cd .
[ Sen. 4, subscore: 1.00 ]: IRT1 is predicted to be an integral membrane protein with a metal-binding domain .
[ Sen. 5, subscore: 1.00 ]: Data base comparisons and Southern blot analysis indicated that IRT1 is a member of a gene family in Arabidopsis .
[ Sen. 7, subscore: 1.00 ]: In Arabidopsis , IRT1 is expressed in roots , is induced by iron deficiency , and has altered regulation in plant lines bearing mutations that affect the iron uptake system .
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| Title: Involvement of NRAMP1 from Arabidopsis thaliana in iron transport .
| | Author: Curie C Alonso JM Le Jean M Ecker JR Briat JF .
| | Journal: Biochem .
J Citation: V : 347 Pt 3 ( ) P : 749-55 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub10769179 Accession (PMID): 10769179 | | Abstract: Nramp genes code for a widely distributed class of proteins involved in a variety of processes , ranging from the control of susceptibility to bacterial infection in mammalian cells and taste behaviour in Drosophila to manganese uptake in yeast Some of the NRAMP proteins in mammals and in yeast are capable of transporting metal ions , including iron .
In plants , iron transport was shown to require a reduction/Fe ( II ) transport system .
In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes .
Here we report the sequence of five NRAMP proteins from A thaliana .
Sequence comparison suggests that there are two classes of NRAMP proteins in plants : A thaliana ( At ) NRAMP1 and Oriza sativa ( Os ) NRAMP1 and 3 ( two rice isologues ) represent one class , and AtNRAMP2-5 and OsNRAMP2 the other .
AtNramp1 and OsNramp1 are able to complement the fet3fet4 yeast mutant defective both in low and high-affinity iron transports , whereas AtNramp2 and OsNramp2 fail to do so .
In addition , AtNramp1 transcript , but not AtNramp2 transcript , accumulates in response to iron deficiency in roots but not in leaves .
Finally , overexpression of AtNramp1 in transgenic A thaliana plants leads to an increase in plant resistance to toxic iron concentration .
Taken together , these results demonstrate that AtNramp1 participates in the control of iron homoeostasis in plants . | Matching Sentences:
[ Sen. 3, subscore: 1.00 ]: In Arabidopsis thaliana this process involves the IRT1 and Fro2 genes .
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| Title: Cloning an iron-regulated metal transporter from rice .
| | Author: Bughio N Yamaguchi H Nishizawa NK Nakanishi H Mori S | | Journal: J Exp . Bot . Citation: V : 53 ( 374 ) P : 1677-82 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12096107 Accession (PMID): 12096107 | | Abstract: Rice cDNA and genomic libraries were screened in order to clone an Fe ( II ) transporter gene .
A cDNA clone highly homologous to the Arabidopsis Fe ( II ) transporter gene IRT1 was isolated from Fe-deficient rice roots .
The cDNA clone was named OsIRT1 .
A genomic clone corresponding to the cDNA was also obtained , sequenced and analysed .
When expressed in yeast cells , OsIRT1 cDNA reversed the growth defects of the yeast iron-uptake mutant .
Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe and Cu-deficiency .
This suggests that OsIRT1 is a functional metal transporter for iron , and is actively engaged in Fe uptake from soils , especially under limiting conditions . | Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: A cDNA clone highly homologous to the Arabidopsis Fe ( II ) transporter gene IRT1 was isolated from Fe-deficient rice roots .
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| Title: Identification of putative target genes to manipulate Fe and Zn concentrations in rice grains .
| | Author: Sperotto RA Boff T Duarte GL Santos LS Grusak MA Fett JP | | Journal: J Plant Physiol Citation: V : 167 P : 1500-6 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20580124 Accession (PMID): 20580124 | | Abstract: Rice is the staple food of half of the worlds population ; however , it is a poor source of essential micronutrients such as Fe and Zn .
Since flag leaves are one of the sources of remobilized metals for developing seeds , the identification of the molecular players that might contribute to the process of metal transport from flag leaves to the seeds may be useful for biofortification purposes .
We analyzed the expression of 25 metal-related genes from rice , including rice homologues for YSLs , NRAMPs , ZIPs , IRT1 , VIT1 ( coding for known or potential metal transporters ) , as well as NASs , FROs and NAC5 ( involved in metal homeostasis ) in flag leaves of eight rice cultivars ( showing contrasting levels of seed Fe and Zn ) during panicle emergence ( R3 ) and grain filling stage ( R5 ) .
The expression level of nine of these genes ( OsYSL6 , OsYSL8 , OsYSL14 , OsNRAMP1 , OsNRAMP7 , OsNRAMP8 , OsNAS1 , OsFRO1 and OsNAC5 ) in flag leaves exhibited significant correlations with Fe and/or Zn concentrations in the seeds .
In this way , our study has provided a short list of putative target genes to manipulate Fe and Zn concentrations in rice grains .
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[ Sen. 3, subscore: 1.00 ]: We analyzed the expression of 25 metal-related genes from rice , including rice homologues for YSLs , NRAMPs , ZIPs , IRT1 , VIT1 ( coding for known or potential metal transporters ) , as well as NASs , FROs and NAC5 ( involved in metal homeostasis ) in flag leaves of eight rice cultivars ( showing contrasting levels of seed Fe and Zn ) during panicle emergence ( R3 ) and grain filling stage ( R5 ) .
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| Title: Ethylene is involved in the regulation of iron homeostasis by regulating the expression of iron-acquisition-related genes in Oryza sativa .
| | Author: Wu J Wang C Zheng L Wang L Chen Y Whelan J Shou H | | Journal: J Exp Bot Citation: V : 62 P : 667-74 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21112958 Accession (PMID): 21112958 | | Abstract: Plants employ two distinct strategies to obtain iron ( Fe ) from the soil .
In Strategy I but not Strategy II plants , Fe limitation invokes ethylene production which regulates Fe deficiency responses .
Oryza sativa ( rice ) is the only graminaceous plant described that possesses a Strategy I-like system for iron uptake as well as the classic Strategy II system .
Ethylene production of rice roots was significantly increased when grown under Fe-depleted conditions .
Moreover , 1-aminocyclopropane-1-carboxylic acid ( ACC ) treatment , a precursor of ethylene , conferred tolerance to Fe deficiency in rice by increasing internal Fe availability .
Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2 , nicotianamine synthases 1 and 2 ( NAS1 and NAS2 ) , yellow-stripe like transporter 15 ( YSL15 ) and iron-regulated transporter ( IRT1 ) indicated that ethylene caused an increase in transcript abundance of both Fe ( II ) and Fe ( III ) -phytosiderophore uptake systems .
RNA interference of OsIRO2 in transgenic rice showed that ethylene acted via this transcription factor to induce the expression of OsNAS1 , OsNAS2 , OsYSL15 , and OsIRT1 .
By contrast , in Hordeum vulgare L ( barley ) , no ethylene production or ethylene-mediated effects of Fe response could be detected .
In conclusion , Fe-limiting conditions increased ethylene production and signalling in rice , which is novel in Strategy II plant species .
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[ Sen. 6, subscore: 1.00 ]: Gene expression analysis of rice iron-regulated bHLH transcription factor OsIRO2 , nicotianamine synthases 1 and 2 ( NAS1 and NAS2 ) , yellow-stripe like transporter 15 ( YSL15 ) and iron-regulated transporter ( IRT1 ) indicated that ethylene caused an increase in transcript abundance of both Fe ( II ) and Fe ( III ) -phytosiderophore uptake systems .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
