Score: 3.00 | Title: Organ-specific alternative transcripts of KNOX family class 2 homeobox genes of rice .
| Author: Ito Y Hirochika H Kurata N | Journal: Gene Citation: V : 288 ( 1-2 ) P : 41-7 Year: 2002 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12034492 Accession (PMID): 12034492 | Abstract: We identified three genes ( HOS58 , HOS59 and HOS66 ) of rice ( Oryza sativa ) , which encode predicted proteins with the KNOX family class 2 homeodomain .
These proteins contain three conserved domains , the KNOX domain , ELK domain and homeodomain , from an N-terminus to a C-terminus .
In addition to similarity of predicted amino acid sequences , these genes showed a similar exon/intron structure . cDNA cloning and reverse transcription-polymerase chain reaction analyses of these genes indicated it issue-specific expression of alternative transcripts .
The expression of the longer mRNAs of HOS58 ( HOS58L ) and HOS59L was detected in all organs examined such as roots , leaf blades , leaf sheaths , flowers and calli , whereas the shorter mRNAs of HOS58 ( HOS58S ) and HOS59S were expressed in leaf blades , leaf sheaths and flowers .
The expression of HOS66L was detected in roots , leaf blades , leaf sheaths and flowers , whereas the expression of HOS66S was detected in roots and flowers .
The alternative transcripts of HOS66 arose by use of alternative transcription start sites .
The longer transcripts contained an exon 1 which encodes an alanine/glycine-rich region , whereas the shorter ones lacked it .
These results suggest that the expression of the alternative transcripts is organ-specific , and their products have different degrees of abilities for activation or repression of transcription . | Matching Sentences: [ Sen. 4, subscore: 2.00 ]: The expression of the longer mRNAs of HOS58 ( HOS58L ) and HOS59L was detected in all organs examined such as roots , leaf blades , leaf sheaths , flowers and calli , whereas the shorter mRNAs of HOS58 ( HOS58S ) and HOS59S were expressed in leaf blades , leaf sheaths and flowers . [ Sen. 1, subscore: 1.00 ]: We identified three genes ( HOS58 , HOS59 and HOS66 ) of rice ( Oryza sativa ) , which encode predicted proteins with the KNOX family class 2 homeodomain .
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