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| Title: Structure and expression of the rice class-I type histone deacetylase genes OsHDAC1-3 : OsHDAC1 overexpression in transgenic plants leads to increased growth rate and altered architecture .
| | Author: Jang IC Pahk YM Song SI Kwon HJ Nahm BH Kim JK .
| | Journal: Plant J Citation: V : 33 ( 3 ) P : 531-41 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12581311 Accession (PMID): 12581311 | | Abstract: Histone deacetylases ( HDACs ) modulate chromatin structure and transcription .
HDACs have been studied as negative regulators in eukaryotic transcription .
We isolated the rice OsHDAC1-3 genes for class I-type histone deacetylases , which are related to the RPD3 family .
The OsHDAC1 gene encoded a protein of approximately 57 kDa that shared 73 . 5 , 72 . 7 , 79 . 9 , and 57 . 1% amino acid sequence identity with the OsHDAC2 , OsHDAC3 , maize RPD3 , and human HDAC1 proteins , respectively .
Genomic structures and Southern blot analyses revealed that OsHDAC1-3 contained seven , six , and seven exons , respectively , and constituted a class I-type family in the rice genome .
OsHDAC1 was expressed at similar levels in the leaves , roots , and callus cells , whereas OsHDAC2 and 3 were expressed in the roots and callus cells , but not in the leaves , exhibiting distinct it issue specificity .
To explore the role of histone deacetylases in transgenic plants , we inserted the OsHDAC1 cDNA fragment into the expression vector Ai : : OsHDAC1 under the control of the ABA-inducible promoter Ai , and transformed the construct into rice .
Levels of mRNA , protein , and HDAC activity were significantly increased in Ai : : OsHDAC1 callus cells .
The amount of tetra-acetylated H4 in the transgenic cells was greatly reduced , and the reduction was abolished upon treatment with trichostatin A These results demonstrate that OsHDAC1 overexpression in transgenic cells both yields enzymatically active HDAC complexes and induces changes in histone acetylation in vivo .
The overexpression leads to a range of novel phenotypes , involving increased growth rate and altered plant architecture , suggesting that OsHDAC1 functions in the genome-wide programming of gene expression . | Matching Sentences:
[ Sen. 4, subscore: 1.00 ]: The OsHDAC1 gene encoded a protein of approximately 57 kDa that shared 73 . 5 , 72 . 7 , 79 . 9 , and 57 . 1% amino acid sequence identity with the OsHDAC2 , OsHDAC3 , maize RPD3 , and human HDAC1 proteins , respectively .
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