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| Title: Proteomic analysis of rice defense response induced by probenazole .
| | Author: Lin YZ Chen HY Kao R Chang SP Chang SJ Lai EM | | Journal: Phytochemistry Citation: V : 69 P : 715-28 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17950386 Accession (PMID): 17950386 | | Abstract: Here , we report the first proteomic analysis of rice defense response induced by probenazole ( PBZ ) , an agricultural chemical that has been widely used to protect rice plants from rice blast and the bacterial blight pathogen .
Two-dimensional gel electrophoresis ( 2-DE ) was utilized to identify a total of 40 protein spots including 9 protein spots that are up-regulated by PBZ and 31 abundant protein spots .
A total of 11 unique proteins from these 9 spots were identified by LC-MS/MS , and the majority of them were classified and/or possessed orthologs in defense-related functions .
Five protein spots with only one protein species identified in each spot appear to be PBZ-regulated proteins .
They are a putative glutathione S-transferase GSTU17 , a putative phenylalanine ammonia-lyase ( PAL , XP_466843 ) , a putative caffeic acid 3-O-methyltransferase ( COMT ) , a putative NADH-ubiquinone oxidoreductase , and a putative glucose-1-phosphate adenyltransferase .
However , the other six protein species identified from the remaining four protein spots could not be conclusively described as PBZ-regulated proteins due to either the co-migration of two protein species in one spot or the presence of one protein species in two spots .
Through real-time reverse transcription polymerase chain reaction ( RT-PCR ) , it was determined that PAL ( XP_466843 ) is likely regulated at the protein level , whereas GSTU17 and COMT were regulated at the mRNA level after PBZ application .
Interestingly , the mRNA transcripts of two PAL paralogs were found to be up-regulated by PBZ .
We propose that PAL , COMT , and GSTU17 are likely to confer PBZ-induced disease resistance via such functions as biosynthesis and transport of flavonoid-type phytoalexin and/or lignin biogenesis .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: They are a putative glutathione S-transferase GSTU17 , a putative phenylalanine ammonia-lyase ( PAL , XP_466843 ) , a putative caffeic acid 3-O-methyltransferase ( COMT ) , a putative NADH-ubiquinone oxidoreductase , and a putative glucose-1-phosphate adenyltransferase .
[ Sen. 7, subscore: 1.00 ]: Through real-time reverse transcription polymerase chain reaction ( RT-PCR ) , it was determined that PAL ( XP_466843 ) is likely regulated at the protein level , whereas GSTU17 and COMT were regulated at the mRNA level after PBZ application .
[ Sen. 9, subscore: 1.00 ]: We propose that PAL , COMT , and GSTU17 are likely to confer PBZ-induced disease resistance via such functions as biosynthesis and transport of flavonoid-type phytoalexin and/or lignin biogenesis .
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