| 8 matches found in 4 documents. Search time: 0.05 seconds. |
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| Title: Identification and mapping of an AFLP marker linked to Gm7 , a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice .
| | Author: Sardesai N Kumar A Rajyashri R Nair S Mohan M | | Journal: Citation: V : 105 ( 5 ) P : 691-698 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12582482 Accession (PMID): 12582482 | | Abstract: We have identified an AFLP marker SA598 that is linked to Gm7 , a gene conferring resistance to biotypes 1 , 2 and 4 of the gall midge ( Orseolia oryzae ) , a major dipteran pest of rice .
A set of PCR primers specific to an RFLP marker , previously identified to be linked to another gall midge resistance gene Gm2 , also amplified a 1 . 5-kb ( F8LB ) fragment that is linked to Gm7 .
Gm7 is a dominant gene and non-allelic to Gm2 .
Hybridization experiments with clones from a YAC library of Nipponbare , a japonica variety , a BAC library of IR-BB21 , an indica variety , and cosmid clones encompassing Gm2 from Phalguna , an indica variety , with F8LB and SA598 as probes , revealed that Gm7 is tightly linked to Gm2 and is located on chromosome 4 of rice .
SA598 was sequenced and the sequence information was used to design sequence-characterized amplified region ( SCAR ) primers .
The potential use of these SCAR primers in marker-aided selection of Gm7 in a rice breeding program has been demonstrated .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: We have identified an AFLP marker SA598 that is linked to Gm7 , a gene conferring resistance to biotypes 1 , 2 and 4 of the gall midge ( Orseolia oryzae ) , a major dipteran pest of rice .
[ Sen. 2, subscore: 1.00 ]: A set of PCR primers specific to an RFLP marker , previously identified to be linked to another gall midge resistance gene Gm2 , also amplified a 1 . 5-kb ( F8LB ) fragment that is linked to Gm7 .
[ Sen. 3, subscore: 1.00 ]: Gm7 is a dominant gene and non-allelic to Gm2 .
[ Sen. 4, subscore: 1.00 ]: Hybridization experiments with clones from a YAC library of Nipponbare , a japonica variety , a BAC library of IR-BB21 , an indica variety , and cosmid clones encompassing Gm2 from Phalguna , an indica variety , with F8LB and SA598 as probes , revealed that Gm7 is tightly linked to Gm2 and is located on chromosome 4 of rice .
[ Sen. 6, subscore: 1.00 ]: The potential use of these SCAR primers in marker-aided selection of Gm7 in a rice breeding program has been demonstrated .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Tagging and mapping of a rice gall midge resistance gene , Gm8 , and development of SCARs for use in marker-aided selection and gene pyramiding .
| | Author: Jain A Ariyadasa R Kumar A Srivastava MN Mohan M Nair S | | Journal: Theor . Appl . Genet . Citation: V : 109 ( 7 ) P : 1377-84 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15322754 Accession (PMID): 15322754 | | Abstract: Using amplified fragment length polymorphisms ( AFLPs ) and random amplified polymorphic DNAs ( RAPDs ) , we have tagged and mapped Gm8 , a gene conferring resistance to the rice gall midge ( Orseolia oryzae ) , a major insect pest of rice , onto rice chromosome 8 .
Using AFLPs , two fragments , AR257 and AS168 , were identified that were linked to the resistant and susceptible phenotypes , respectively .
Another resistant phenotype-specific marker , AP19 ( 587 ) , was also identified using RAPDs .
SCAR primers based on the sequence of the fragments AR257 and AS168 failed to reveal polymorphism between the resistant and the susceptible parents .
However , PCR using primers based on the regions flanking AR257 revealed polymorphism that was phenotype-specific .
In contrast , PCR carried out using primers flanking the susceptible phenotype-associated fragment AS168 produced a monomorphic fragment .
Restriction digestion of these monomorphic fragments revealed polymorphism between the susceptible and resistant parents .
Nucleotide BLAST searches revealed that the three fragments show strong homology to rice PAC and BAC clones that formed a contig representing the short arm of chromosome 8 .
PCR amplification using the above-mentioned primers on a larger population , derived from a cross between two indica rice varieties , Jhitpiti ( resistant parent ) and TN1 ( susceptible parent ) , showed that there is a tight linkage between the markers and the Gm8 locus .
These markers , therefore , have potential for use in marker-aided selection and pyramiding of Gm8 along with other previously tagged gall midge resistance genes [ Gm2 , Gm4 ( t ) , and Gm7 ] . | Matching Sentences:
[ Sen. 10, subscore: 1.00 ]: These markers , therefore , have potential for use in marker-aided selection and pyramiding of Gm8 along with other previously tagged gall midge resistance genes [ Gm2 , Gm4 ( t ) , and Gm7 ] .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Sensory characteristics and consumer acceptability of beef stock containing glutathione Maillard reaction products prepared at various conditions .
| | Author: Kwon GY Hong JH Kim YS Lee SM Kim KO | | Journal: J Food Sci Citation: V : 76 P : S1-7 Year: 2011 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21535706 Accession (PMID): 21535706 | | Abstract: The sensory characteristics and consumer acceptability of beef soup samples containing 9 types of glutathione Maillard reaction products ( GMRPs ) were investigated to examine the effects of the GMRPs produced under different reaction conditions on the flavor of the beef soup .
The sensory characteristics of the beef stocks were examined using descriptive analysis .
In consumer testing , 50 consumers evaluated the overall acceptability and flavor intensities of beef odor , salty taste , beef flavor , and seasoning flavor in the beef soup samples .
It was found that the reaction conditions , including sugar type and pH , affected the sensory characteristics of the beef stock containing the GMRPs .
The samples containing the GMRPs reacted at pH 7 were characterized with strong beef flavor , chestnut flavor , and cooked rice flavor .
However , the GMRP reacted with xylose at pH 7 ( XM7 ) was significantly stronger in beef-related sensory characteristics than the GMRPs reacted with glucose or fructose at pH 7 ( GM7 and FM7 ) .
The samples containing the GMRPs reacted at pH 3 had strong acid-related attributes whereas the GMRPs reacted at pH 11 exhibited strong sulfur-related attributes and a bitter taste .
Overall , the beef soup containing XM7 , which was perceived as having a strong beef odor and flavor , was rated the highest consumer acceptability score .
This suggests that XM7 has feasibility as a flavor enhancer .
To elucidate its effectiveness further , it is required to apply XM7 in various food systems at varying levels and to compare its flavor enhancing effects with other flavor enhancers such as monosodium L-glutamate in future studies .
Practical Application : This study characterized sensory attributes of glutathione Maillard reaction products ( GMRPs ) reacted under various conditions and evaluated their potential as a flavor enhancer by examining consumer acceptability of beef stock containing the GMRPs .
This study showed that the GMRP reacted with xylose at pH 7 had strong 71 beef flavor and the highest consumer acceptability score .
The results of this study will provide valuable information for understanding sensory aspect of flavors generated by Maillard reaction of GSH and sugars , since most studies on Maillard reaction focused on chemical reactions .
Also , the outcome of this study will help flavor and food industries efforts to develop a new flavor enhancer for use in a variety of processed food products .
| Matching Sentences:
[ Sen. 6, subscore: 1.00 ]: However , the GMRP reacted with xylose at pH 7 ( XM7 ) was significantly stronger in beef-related sensory characteristics than the GMRPs reacted with glucose or fructose at pH 7 ( GM7 and FM7 ) .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Identification and mapping of an AFLP marker linked to Gm7 , a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice .
| | Author: Sardesai N Kumar A Rajyashri R Nair S Mohan M | | Journal: Citation: V : 105 ( 5 ) P : 691-698 Year: 2002 Type: ARTICLE | | Literature: oryza Field: title Doc ID: pub12582482 Accession (PMID): 12582482 | | Abstract: We have identified an AFLP marker SA598 that is linked to Gm7 , a gene conferring resistance to biotypes 1 , 2 and 4 of the gall midge ( Orseolia oryzae ) , a major dipteran pest of rice .
A set of PCR primers specific to an RFLP marker , previously identified to be linked to another gall midge resistance gene Gm2 , also amplified a 1 . 5-kb ( F8LB ) fragment that is linked to Gm7 .
Gm7 is a dominant gene and non-allelic to Gm2 .
Hybridization experiments with clones from a YAC library of Nipponbare , a japonica variety , a BAC library of IR-BB21 , an indica variety , and cosmid clones encompassing Gm2 from Phalguna , an indica variety , with F8LB and SA598 as probes , revealed that Gm7 is tightly linked to Gm2 and is located on chromosome 4 of rice .
SA598 was sequenced and the sequence information was used to design sequence-characterized amplified region ( SCAR ) primers .
The potential use of these SCAR primers in marker-aided selection of Gm7 in a rice breeding program has been demonstrated .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Identification and mapping of an AFLP marker linked to Gm7 , a gall midge resistance gene and its conversion to a SCAR marker for its utility in marker aided selection in rice .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
