Score: 4.00 |
|---|
| Title: Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene .
| | Author: Qu LQ Wei XL Satoh H Kumamaru T Ogawa M Takaiwa F | | Journal: Theor . Appl . Genet . Citation: V : 107 ( 1 ) P : 20-5 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12835929 Accession (PMID): 12835929 | | Abstract: The rice ( Oryza sativa L ) mutant of glu4a , lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased ( alpha-1H/alpha-2L ) , was used in this study .
Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6 . 71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6 . 50/alpha-1 .
Experiments were conducted to identify the relationships between the mutated polypeptides of the mutant and to illustrate the mutation mechanism of the allele .
Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6 . 50/alpha-1 of high homology with the deleted pI6 . 71/alpha-2 polypeptide which was encoded by glu4 ( GluA-1 ) .
The nucleotide sequence revealed that the iso-electric point variation of the pI6 . 50/alpha-1 polypeptide was caused by a point mutation with nucleotide replacement at the variable region of the gene .
These results suggested the possibility of altering glutelin quality by using single gene mutation . | Matching Sentences:
[ Sen. 4, subscore: 2.00 ]: Peptide mapping and amino-acid sequence analyses revealed that the newly formed glu4a encoded polypeptide pI6 . 50/alpha-1 of high homology with the deleted pI6 . 71/alpha-2 polypeptide which was encoded by glu4 ( GluA-1 ) .
[ Sen. 1, subscore: 1.00 ]: The rice ( Oryza sativa L ) mutant of glu4a , lacking the glutelin alpha-2 subunit while the alpha-1 subunit increased ( alpha-1H/alpha-2L ) , was used in this study .
[ Sen. 2, subscore: 1.00 ]: Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6 . 71/alpha-2 encoded by glu4 while forming a new polypeptide of pI6 . 50/alpha-1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
|---|
| Title: Inheritance of alleles for glutelin alpha-2 subunit genes in rice and identification of their corresponding cDNA clone .
| | Author: Qu Q Wei L Satoh H Kumamaru T Ogawa M Takaiwa F | | Journal: Citation: V : 105 ( 8 ) P : 1099-1108 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12582887 Accession (PMID): 12582887 | | Abstract: Rice glutelins consist of acidic ( alpha ) and basic ( beta ) subunits which are further separated into three polypeptide components assigned as alpha-1 , alpha-2 , and alpha-3 subunit components and beta-1 , beta-2 and beta-3 subunit components .
Nine rice mutant lines with a decreased amount of the glutelin alpha-2 subunit component ( alpha-2L ) were obtained by screening about 6 , 800 potential mutant lines derived from the fertilized egg treatment with N-methyl-N-nitrosourea ( MNU ) using the SDS-PAGE method .
The mutants were classified into three types of the increased alpha-1 subunit ( alpha-1H/alpha-2L ) , the decreased beta-2 subunit ( beta-2L/alpha-2L ) and the increased alpha-3 subunit ( alpha-3H/alpha-2L ) represented by EM278 , CM1707 and EM659 , respectively .
Iso-electric focus ( IEF ) analysis revealed that all of the mutants had an extremely low amount of a polypeptide with a 6 . 71 pI value , whereas a polypeptide with either a 6 . 50 pI value or with a 6 . 90 pI value increased significantly in alpha-1H/alpha-2L mutants or in alpha-3H/alpha-2L mutants , respectively .
The beta-2L/alpha-2L mutants had a decreased amount of a basic polypeptide with a 8 . 74 pI value .
Genetic analysis revealed that the three types of mutants were controlled by a single incomplete dominant gene respectively , and the three are alleles .
The gene was temporarily named glu4 , which was found to be located on chromosome 1 linked with the eg and spl6 genes .
Two-dimensional electrophoresis analysis revealed that the glu4 encoded polypeptides of pI 6 . 71/alpha-2 and pI 8 . 74/beta-2 .
Amino acid sequence analysis suggested that the mutated acidic polypeptide was the product of a GluA subfamily gene .
Northern and RT-PCR analyses revealed that glu4 corresponded to the GluA-1 gene .
| Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: The gene was temporarily named glu4 , which was found to be located on chromosome 1 linked with the eg and spl6 genes .
[ Sen. 8, subscore: 1.00 ]: Two-dimensional electrophoresis analysis revealed that the glu4 encoded polypeptides of pI 6 . 71/alpha-2 and pI 8 . 74/beta-2 .
[ Sen. 10, subscore: 1.00 ]: Northern and RT-PCR analyses revealed that glu4 corresponded to the GluA-1 gene .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
