| 5 matches found in 4 documents. Search time: 0.175 seconds. |
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| Title: Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots .
| | Author: Ishiyama K Inoue E Tabuchi M Yamaya T Takahashi H | | Journal: Plant Cell Physiol .
Citation: V : 45 ( 11 ) P : 1640-7 Year: 2004 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub15574840 Accession (PMID): 15574840 | | Abstract: Rice plants in paddy fields prefer to utilize ammonium as a major nitrogen source .
Glutamine synthetase ( GS ) serves for assimilation of ammonium in rice root , and ameliorates the toxic effect of ammonium excess .
Among the three isoenzymes of the cytosolic GS1 gene family in rice , OsGLN1 ; 1 and OsGLN1 ; 2 were abundantly expressed in roots .
Analysis of the purified enzymes showed that OsGLN1 ; 1 and OsGLN1 ; 2 can be classified into high-affinity subtypes with relatively high V ( max ) values , as compared with the major high-affinity isoenzyme , GLN1 ; 1 , in Arabidopsis .
Low-affinity forms of GS1 comparable to those in Arabidopsis ( GLN1 ; 2 and GLN1 ; 3 ) were absent in rice roots .
The OsGLN1 ; 1 and OsGLN1 ; 2 transcripts showed reciprocal responses to ammonium supply in the surface cell layers of roots .
OsGLN1 ; 1 accumulated in dermatogen , epidermis and exodermis under nitrogen-limited condition .
By contrast , OsGLN1 ; 2 was abundantly expressed in the same cell layers under nitrogen-sufficient conditions , replenishing the loss of OsGLN1 ; 1 following ammonium treatment .
Within the central cylinder of elongating zone , OsGLN1 ; 1 and OsGLN1 ; 2 were both induced by ammonium , which was distinguishable from the response observed in the surface cell layers .
The high-capacity Gln synthetic activities of OsGLN1 ; 1 and OsGLN1 ; 2 facilitate active ammonium assimilation in specific cell types in rice roots . | Matching Sentences:
[ Sen. 5, subscore: 2.00 ]: Low-affinity forms of GS1 comparable to those in Arabidopsis ( GLN1 ; 2 and GLN1 ; 3 ) were absent in rice roots .
[ Sen. 4, subscore: 1.00 ]: Analysis of the purified enzymes showed that OsGLN1 ; 1 and OsGLN1 ; 2 can be classified into high-affinity subtypes with relatively high V ( max ) values , as compared with the major high-affinity isoenzyme , GLN1 ; 1 , in Arabidopsis .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: Biochemical and biological properties of DNA photolyases derived from utraviolet-sensitive rice cultivars .
| | Author: Yamamoto A Hirouchi T Mori T Teranishi M Hidema J Morioka H Kumagai T Yamamoto K | | Journal: Genes Genet Syst Citation: V : 82 P : 311-9 Year: 2007 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub17895582 Accession (PMID): 17895582 | | Abstract: Class I and class II CPD photolyases are enzymes which repair pyrimidine dimers using visible light .
A detailed characterization of class I CPD photolyases has been carried out , but little is known about the class II enzymes .
Photolyases from rice are suitable for functional analyses because systematic breeding for long periods in Asian countries has led to the selection of naturally occurring mutations in the CPD photolyase gene .
We report the biochemical characterization of rice mutant CPD photolyases purified as GST-form from Escherichia coli .
We identified three amino acid changes , Gln126Arg , Gly255Ser , and Gln296His , among which Gln but not His at 296 is important for complementing phr-defective E coli , binding UV-damage in E coli , and binding thymine dimers in vitro .
The photolyase with Gln at 296 has an apoenzyme : FAD ratio of 1 : 0 . 5 and that with His at 296 has an apoenzyme : FAD ratio of 1 : 0 . 12-0 . 25 , showing a role for Gln at 296 in the binding of FAD not in the binding of thymine dimer .
Concerning Gln or Arg at 126 , the biochemical activity of the photolyases purified from E coli and complementing activity for phr-defective E coli are similarly proficient .
However , the sensitivity to UV of cultivars differs depending on whether Gln or Arg is at 126 .
The role of Gln and Arg at 126 for photoreactivation in rice is discussed .
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[ Sen. 5, subscore: 1.00 ]: We identified three amino acid changes , Gln126Arg , Gly255Ser , and Gln296His , among which Gln but not His at 296 is important for complementing phr-defective E coli , binding UV-damage in E coli , and binding thymine dimers in vitro .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The 3D structure of the defense-related rice protein Pir7b predicted by homology modeling and ligand binding studies .
| | Author: Luo Q Han WW Zhou YH Yao Y Li ZS | | Journal: J Mol Model Citation: V : 14 P : 559-69 Year: 2008 Type: In-Process | | Literature: oryza Field: abstract Doc ID: pub18449577 Accession (PMID): 18449577 | | Abstract: To better understand the ligand-binding mechanism of protein Pir7b , important part in detoxification of a pathogen-derived compound against Pyricularia oryzae , a 3D structure model of protein Pir7b was constructed based on the structure of the template SABP2 .
Three substrates were docking to this protein , two of them were proved to be active , and some critical residues are identified , which had not been confirmed by the experiments .
His87 and Leu17 considered as oxyanion hole contribute to initiating the Ser86 nucleophilic attack .
Gln187 and Asp139 can form hydrogen bonds with the anilid group to maintain the active binding orientation with the substrates .
The docking model can well interpret the specificity of protein Pir7b towards the anilid moiety of the substrates and provide valuable structure information about the ligand binding to protein Pir7b .
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[ Sen. 4, subscore: 1.00 ]: Gln187 and Asp139 can form hydrogen bonds with the anilid group to maintain the active binding orientation with the substrates .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: A cost-effective high-resolution melting approach using the EvaGreen dye for DNA polymorphism detection and genotyping in plants .
| | Author: Li YD Chu ZZ Liu XG Jing HC Liu YG Hao DY | | Journal: J Integr Plant Biol Citation: V : 52 P : 1036-42 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub21106003 Accession (PMID): 21106003 | | Abstract: High-resolution melting ( HRM ) analysis relies on the use of fluorescent dyes , such as LCGreen , ResoLight , and SYTO9 , which bind in a saturated manner to the double-stranded DNAs .
These dyes are expensive in use and may not be affordable when dealing with a large quantity of samples .
EvaGreen is a much cheaper DNA helix intercalating dye and has been used in quantitative real-time polymerase chain reaction ( PCR ) and post-PCR DNA melt curve analysis .
Here we report on the development of an EvaGreen-based HRM analysis and its performance , in comparison with the popular LCGreen-based HRM analysis , in detection of DNA polymorphism in plants .
We found that various polymorphisms ranged from single nucleotide polymorphisms ( SNPs ) to Indels were equally detected by using EvaGreen or LCGreen-based HRM .
EvaGreen dye was sensitive enough in discovery of SNPs in fivefold pooled samples .
Using this economical dye we successfully identified multiple novel mutant alleles of Gln1-3 gene , which produces a cytosolic glutamine synthetase isoenzyme ( GS1 ) , in a maize ethyl methanesulfonate ( EMS ) -mutagenized library , and genotyped rice mapping populations with SNP markers .
The current results suggest that EvaGreen is a promising dye for HRM analysis for its ease to use and cost effectiveness .
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[ Sen. 7, subscore: 1.00 ]: Using this economical dye we successfully identified multiple novel mutant alleles of Gln1-3 gene , which produces a cytosolic glutamine synthetase isoenzyme ( GS1 ) , in a maize ethyl methanesulfonate ( EMS ) -mutagenized library , and genotyped rice mapping populations with SNP markers .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
