| 17 matches found in 7 documents. Search time: 0.333 seconds. |
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| Title: A novel approach for developing resistance in rice against phloem limited viruses by antagonizing the phloem feeding hemipteran vectors .
| | Author: Saha P Dasgupta I Das S | | Journal: Plant Mol . Biol . Citation: V : 62 ( 4-5 ) P : 735-52 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16941213 Accession (PMID): 16941213 | | Abstract: Rice production is known to be severely affected by virus transmitting rice pests , brown planthopper ( BPH ) and green leafhopper ( GLH ) of the order hemiptera , feeding by phloem abstraction .
ASAL , a novel lectin from leaves of garlic ( Allium sativum ) was previously demonstrated to be toxic towards hemipteran pests when administered in artificial diet as well as in ASAL expressing transgenic plants .
In this report ASAL was targeted under the control of phloem-specific Agrobacterium rolC and rice sucrose synthase-1 ( RSs1 ) promoters at the insect feeding site into popular rice cultivar , susceptible to hemipteran pests .
PCR , Southern blot and C-PRINS analyses of transgenic plants have confirmed stable T-DNA integration and the transgenes were co-segregated among self-fertilized progenies .
The T ( 0 ) and T ( 1 ) plants , harbouring single copy of intact T-DNA expression cassette , exhibit stable expression of ASAL in northern and western blot analyses .
ELISA showed that the level of expressed ASAL was as high as 1 . 01% of total soluble protein .
Immunohistofluorescence localization of ASAL depicted the expected expression patterns regulated by each promoter type .
In-planta bioassay studies revealed that transgenic ASAL adversely affect survival , growth and population of BPH and GLH .
GLH resistant T ( 1 ) plants were further evaluated for the incidence of tungro disease , caused by co-infection of GLH vectored Rice tungro bacilliform virus ( RTBV ) and Rice tungro spherical virus ( RTSV ) , which appeared to be dramatically reduced .
The result presented here is the first report of such GLH mediated resistance to infection by RTBV/RTSV in ASAL expressing transgenic rice plant . | Matching Sentences:
[ Sen. 9, subscore: 2.00 ]: GLH resistant T ( 1 ) plants were further evaluated for the incidence of tungro disease , caused by co-infection of GLH vectored Rice tungro bacilliform virus ( RTBV ) and Rice tungro spherical virus ( RTSV ) , which appeared to be dramatically reduced .
[ Sen. 1, subscore: 1.00 ]: Rice production is known to be severely affected by virus transmitting rice pests , brown planthopper ( BPH ) and green leafhopper ( GLH ) of the order hemiptera , feeding by phloem abstraction .
[ Sen. 8, subscore: 1.00 ]: In-planta bioassay studies revealed that transgenic ASAL adversely affect survival , growth and population of BPH and GLH .
[ Sen. 10, subscore: 1.00 ]: The result presented here is the first report of such GLH mediated resistance to infection by RTBV/RTSV in ASAL expressing transgenic rice plant .
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Score: 3.00 |
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| Title: Resistance to green leafhopper ( Nephotettix virescens ) and brown planthopper ( Nilaparvata lugens ) in transgenic rice expressing snowdrop lectin ( Galanthus nivalis agglutinin ; GNA ) .
| | Author: Foissac X Thi Loc N Christou P Gatehouse AM Gatehouse JA .
| | Journal: Citation: V : 46 ( 4 ) P : 573-583 Year: 2000 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12770221 Accession (PMID): 12770221 | | Abstract: Transgenic rice plants expressing snowdrop lectin ( Galanthus nivalis agglutinin ; GNA ) were screened for resistance to green leafhopper ( Nephotettix virescens ; GLH ) , a major homopteran pest of rice .
Survival was reduced by 29% and 53% ( P<0 . 05 ) respectively , on plants where GNA expression was it issue-specific ( phloem and epidermal layer ) or constitutive .
Similar levels of resistance in GNA-expressing transgenic rice were previously reported for rice brown planthopper ( Nilaparvata lugens ; BPH ) .
GNA binding to glycoproteins in gut it issues showed that BPH contained more "receptors" than GLH , and that the binding affinity was stronger , particularly in the midgut .
Subsequent toxicity of GNA is thus unlikely to be directly related to the amount of lectin bound .
GNA was not detected in the honeydew of either insect species when they were fed on GNA-expressing plants , in contrast to results from artificial diet studies .
This result suggests that GNA is not being delivered to the insect efficiently .
When offered a free choice vs control plants , BPH nymphs tended to avoid plants expressing GNA ; avoidance was less pronounced and took longer to develop on plants where GNA expression was it issue-specific , In contrast to BPH , GLH nymphs were attracted to plants expressing GNA , whether constitutively or in a it issue-specific manner .
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[ Sen. 1, subscore: 1.00 ]: Transgenic rice plants expressing snowdrop lectin ( Galanthus nivalis agglutinin ; GNA ) were screened for resistance to green leafhopper ( Nephotettix virescens ; GLH ) , a major homopteran pest of rice .
[ Sen. 4, subscore: 1.00 ]: GNA binding to glycoproteins in gut it issues showed that BPH contained more "receptors" than GLH , and that the binding affinity was stronger , particularly in the midgut .
[ Sen. 8, subscore: 1.00 ]: When offered a free choice vs control plants , BPH nymphs tended to avoid plants expressing GNA ; avoidance was less pronounced and took longer to develop on plants where GNA expression was it issue-specific , In contrast to BPH , GLH nymphs were attracted to plants expressing GNA , whether constitutively or in a it issue-specific manner .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 3.00 |
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| Title: Transgenic rice expressing Allium sativum leaf agglutinin ( ASAL ) exhibits high-level resistance against major sap-sucking pests .
| | Author: Yarasi B Sadumpati V Immanni CP Vudem DR Khareedu VR | | Journal: BMC Plant Biol Citation: V : 8 P : 102 Year: 2008 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub18854007 Accession (PMID): 18854007 | | Abstract: BACKGROUND : Rice ( Oryza sativa ) productivity is adversely impacted by numerous biotic and abiotic factors .
An approximate 52% of the global production of rice is lost annually owing to the damage caused by biotic factors , of which approximately 21% is attributed to the attack of insect pests .
In this paper we report the isolation , cloning and characterization of Allium sativum leaf agglutinin ( asal ) gene , and its expression in elite indica rice cultivars using Agrobacterium-mediated genetic transformation method .
The stable transgenic lines , expressing ASAL , showed explicit resistance against major sap-sucking pests .
RESULTS : Allium sativum leaf lectin gene ( asal ) , coding for mannose binding homodimeric protein ( ASAL ) from garlic plants , has been isolated and introduced into elite indica rice cultivars susceptible to sap-sucking insects , viz . , brown planthopper ( BPH ) , green leafhopper ( GLH ) and whitebacked planthopper ( WBPH ) .
Embryogenic calli of rice were co-cultivated with Agrobacterium harbouring pSB111 super-binary vector comprising garlic lectin gene asal along with the herbicide resistance gene bar , both under the control of CaMV35S promoter .
PCR and Southern blot analyses confirmed stable integration of transgenes into the genomes of rice plants .
Northern and western blot analyses revealed expression of ASAL in different transgenic rice lines .
In primary transformants , the level of ASAL protein , as estimated by enzyme-linked immunosorbent assay , varied between 0 . 74% and 1 . 45% of the total soluble proteins .
In planta insect bioassays on transgenic rice lines revealed potent entomotoxic effects of ASAL on BPH , GLH and WBPH insects , as evidenced by significant decreases in the survival , development and fecundity of the insects .
CONCLUSION : In planta insect bioassays were carried out on asal transgenic rice lines employing standard screening techniques followed in conventional breeding for selection of insect resistant plants .
The ASAL expressing rice plants , bestowed with high entomotoxic effects , imparted appreciable resistance against three major sap-sucking insects .
Our results amply demonstrate that transgenic indica rice harbouring asal exhibit surpassing resistance against BPH , GLH and WBPH insects .
The prototypic asal transgenic rice lines appear promising for direct commercial cultivation besides serving as a potential genetic resource in recombination breeding .
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[ Sen. 5, subscore: 1.00 ]: RESULTS : Allium sativum leaf lectin gene ( asal ) , coding for mannose binding homodimeric protein ( ASAL ) from garlic plants , has been isolated and introduced into elite indica rice cultivars susceptible to sap-sucking insects , viz . , brown planthopper ( BPH ) , green leafhopper ( GLH ) and whitebacked planthopper ( WBPH ) .
[ Sen. 10, subscore: 1.00 ]: In planta insect bioassays on transgenic rice lines revealed potent entomotoxic effects of ASAL on BPH , GLH and WBPH insects , as evidenced by significant decreases in the survival , development and fecundity of the insects .
[ Sen. 13, subscore: 1.00 ]: Our results amply demonstrate that transgenic indica rice harbouring asal exhibit surpassing resistance against BPH , GLH and WBPH insects .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Transgenic indica rice resistant to sap-sucking insects .
| | Author: Nagadhara D Ramesh S Pasalu IC Rao YK Krishnaiah NV Sarma NP Bown DP Gatehouse JA Reddy VD Rao KV .
| | Journal: Citation: V : 1 ( 3 ) P : 231-240 Year: 2003 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub17156035 Accession (PMID): 17156035 | | Abstract: Agrobacterium-mediated genetic transformation has been optimized in indica rice susceptible to sap-sucking insects , viz . , brown planthopper ( BPH ) and green leafhopper ( GLH ) .
Snowdrop lectin gene ( gna ) from Galanthus nivalis , driven by phloem-specific rice-sucrose-synthase promoter , along with herbicide resistance gene ( bar ) driven by CaMV 35S promoter , was employed for genetic transformation .
Embryogenic calli - after co-cultivation with Agrobacterium strain LBA4404 harbouring Ti plasmid pSB111-bar-gna -were selected on the medium containing phosphinothricin .
PCR and Southern blot analyses confirmed the stable integration of both the genes into genomes of transgenic ( T ( 0 ) ) rice plants .
Northern and Western blot analyses revealed the expression of gna in the transgenic plants .
In the T ( 1 ) and T ( 2 ) generations , the gna and bar transgenes showed co-segregation at a ratio of 3 : 1 .
Plant progenies expressing gna , in T ( 1 ) and T ( 2 ) , exhibited substantial resistance against BPH and GLH pests .
This is the first report dealing with transgenic indica rice exhibiting high resistance to both insects .
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[ Sen. 1, subscore: 1.00 ]: Agrobacterium-mediated genetic transformation has been optimized in indica rice susceptible to sap-sucking insects , viz . , brown planthopper ( BPH ) and green leafhopper ( GLH ) .
[ Sen. 7, subscore: 1.00 ]: Plant progenies expressing gna , in T ( 1 ) and T ( 2 ) , exhibited substantial resistance against BPH and GLH pests .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 2.00 |
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| Title: Selectable antibiotic resistance marker gene-free transgenic rice harbouring the garlic leaf lectin gene exhibits resistance to sap-sucking planthoppers .
| | Author: Sengupta S Chakraborti D Mondal HA Das S | | Journal: Plant Cell Rep Citation: V : 29 P : 261-71 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20094886 Accession (PMID): 20094886 | | Abstract: Rice , the major food crop of world is severely affected by homopteran sucking pests .
We introduced coding sequence of Allium sativum leaf agglutinin , ASAL , in rice cultivar IR64 to develop sustainable resistance against sap-sucking planthoppers as well as eliminated the selectable antibiotic-resistant marker gene hygromycin phosphotransferase ( hpt ) exploiting cre/lox site-specific recombination system .
An expression vector was constructed containing the coding sequence of ASAL , a potent controlling agent against green leafhoppers ( GLH , Nephotettix virescens ) and brown planthopper ( BPH , Nilaparvata lugens ) .
The selectable marker ( hpt ) gene cassette was cloned within two lox sites of the same vector .
Alongside , another vector was developed with chimeric cre recombinase gene cassette .
Reciprocal crosses were performed between three single-copy T ( 0 ) plants with ASAL lox-hpt-lox T-DNA and three single-copy T ( 0 ) plants with cre-bar T-DNA .
Marker gene excisions were detected in T ( 1 ) hybrids through hygromycin sensitivity assay .
Molecular analysis of T ( 1 ) plants exhibited 27 . 4% recombination efficiency .
T ( 2 ) progenies of L03C04 ( 1 ) hybrid parent showed 25% cre negative ASAL-expressing plants .
Northern blot , western blot and ELISA showed significant level of ASAL expression in five marker-free T ( 2 ) progeny plants .
In planta bioassay of GLH and BPH performed on these T ( 2 ) progenies exhibited radical reduction in survivability and fecundity compared with the untransformed control plants .
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[ Sen. 3, subscore: 1.00 ]: An expression vector was constructed containing the coding sequence of ASAL , a potent controlling agent against green leafhoppers ( GLH , Nephotettix virescens ) and brown planthopper ( BPH , Nilaparvata lugens ) .
[ Sen. 11, subscore: 1.00 ]: In planta bioassay of GLH and BPH performed on these T ( 2 ) progenies exhibited radical reduction in survivability and fecundity compared with the untransformed control plants .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
