| 16 matches found in 6 documents. Search time: 1.929 seconds. |
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Score: 11.00 |
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| Title: Characterization and fine mapping of the glabrous leaf and hull mutants ( gl1 ) in rice ( Oryza sativa L ) .
| | Author: Li W Wu J Weng S Zhang D Zhang Y Shi C | | Journal: Plant Cell Rep Citation: V : 29 P : 617-27 Year: 2010 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub20376671 Accession (PMID): 20376671 | | Abstract: The glabrous leaf and hull ( gl1 ) mutants were isolated from M ( 2 ) generation of indica cultivar 93-11 .
These mutants produced smooth leaves and hairless glumes under normal growth conditions .
By analyzing through scanning electron microscope , it was revealed that the leaf trichomes , including macro and micro hairs , were deficient in these mutants .
Genetic analysis indicated that the mutation was controlled by a single recessive gene .
Using nine SSR markers and one InDel marker , the gl1 gene was mapped between RM1200 and RM2010 at the short arm of chromosome 5 , which was consistent with the mapping of gl1 in previous studies .
To facilitate the map-based cloning of the gl1 gene , 12 new InDel markers were developed .
A high-resolution genetic and physical map was constructed by using 1 , 396 mutant individuals of F ( 2 ) mapping population .
Finally , the gl1 was fine mapped in 54-kb region containing 10 annotated genes .
Cloning and sequencing of the target region from four gl1 mutants ( gl1-1 , gl1-2 , gl1-3 and gl1-4 ) and four glabrous rice varieties ( Jackson , Jefferson , Katy and Lemont ) all showed that the same single point mutation ( A-->T ) occurred in the 5-untranslated region ( UTR ) of the locus Os05g0118900 ( corresponding to the 3-UTR of STAR2 ) .
RT-PCR analysis of the locus Os05g0118900 revealed that its mRNA expression level was normal in gl1 mutant .
RNA secondary structure prediction showed that the single point mutation resulted in a striking RNA conformational change .
These results suggest that the single point mutation is most likely responsible for the glabrous leaf and hull phenotypes in rice .
| Matching Sentences:
[ Sen. 9, subscore: 5.00 ]: Cloning and sequencing of the target region from four gl1 mutants ( gl1-1 , gl1-2 , gl1-3 and gl1-4 ) and four glabrous rice varieties ( Jackson , Jefferson , Katy and Lemont ) all showed that the same single point mutation ( A-->T ) occurred in the 5-untranslated region ( UTR ) of the locus Os05g0118900 ( corresponding to the 3-UTR of STAR2 ) .
[ Sen. 5, subscore: 2.00 ]: Using nine SSR markers and one InDel marker , the gl1 gene was mapped between RM1200 and RM2010 at the short arm of chromosome 5 , which was consistent with the mapping of gl1 in previous studies .
[ Sen. 1, subscore: 1.00 ]: The glabrous leaf and hull ( gl1 ) mutants were isolated from M ( 2 ) generation of indica cultivar 93-11 .
[ Sen. 6, subscore: 1.00 ]: To facilitate the map-based cloning of the gl1 gene , 12 new InDel markers were developed .
[ Sen. 8, subscore: 1.00 ]: Finally , the gl1 was fine mapped in 54-kb region containing 10 annotated genes .
[ Sen. 10, subscore: 1.00 ]: RT-PCR analysis of the locus Os05g0118900 revealed that its mRNA expression level was normal in gl1 mutant .
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Score: 4.00 |
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| Title: Characterization of Glossy1-homologous genes in rice involved in leaf wax accumulation and drought resistance .
| | Author: Islam MA Du H Ning J Ye H Xiong L | | Journal: Plant Mol Biol Citation: V : P : Year: 2009 Type: Publisher | | Literature: oryza Field: abstract Doc ID: pub19322663 Accession (PMID): 19322663 | | Abstract: The outermost surfaces of plants are covered with an epicuticular wax layer that provides a primary waterproof barrier and protection against different environmental stresses .
Glossy 1 ( GL1 ) is one of the reported genes controlling wax synthesis .
This study analyzed GL1-homologous genes in Oryza sativa and characterized the key members of this family involved in wax synthesis and stress resistance .
Sequence analysis revealed 11 homologous genes of GL1 in rice , designated OsGL1-1 to OsGL1-11 .
OsGL1-1 , -2 and -3 are closely related to GL1 .
OsGL1-4 , -5 , -6 , and -7 are closely related to Arabidopsis CER1 that is involved in cuticular wax biosynthesis .
OsGL1-8 , -9 , -10 and -11 are closely related to SUR2 encoding a putative sterol desaturase also involved in epicuticular wax biosynthesis .
These genes showed variable expression levels in different it issues and organs of rice , and most of them were induced by abiotic stresses .
Compared to the wild type , the OsGL1-2-over-expression rice exhibited more wax crystallization and a thicker epicuticular layer ; while the mutant of this gene showed less wax crystallization and a thinner cuticular layer .
Chlorophyll leaching experiment suggested that the cuticular permeability was decreased and increased in the over-expression lines and the mutant , respectively .
Quantification analysis of wax composition by GC-MS revealed a significant reduction of total cuticular wax in the mutant and increase of total cuticular wax in the over-expression plants .
Compared to the over-expression and wild type plants , the osgl1-2 mutant was more sensitive to drought stress at reproductive stage , suggesting an important role of this gene in drought resistance .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: Glossy 1 ( GL1 ) is one of the reported genes controlling wax synthesis .
[ Sen. 3, subscore: 1.00 ]: This study analyzed GL1-homologous genes in Oryza sativa and characterized the key members of this family involved in wax synthesis and stress resistance .
[ Sen. 4, subscore: 1.00 ]: Sequence analysis revealed 11 homologous genes of GL1 in rice , designated OsGL1-1 to OsGL1-11 .
[ Sen. 5, subscore: 1.00 ]: OsGL1-1 , -2 and -3 are closely related to GL1 .
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Score: 3.00 |
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| Title: The glossy1 locus of maize and an epidermis-specific cDNA from Kleinia odora define a class of receptor-like proteins required for the normal accumulation of cuticular waxes .
| | Author: Hansen JD Pyee J Xia Y Wen TJ Robertson DS Kolattukudy PE Nikolau BJ Schnable PS .
| | Journal: Plant Physiol .
Citation: V : 113 ( 4 ) P : 1091-100 Year: 1997 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub9112770 Accession (PMID): 9112770 | | Abstract: Mutations at the glossy1 ( gl1 ) locus of maize ( Zea mays L ) quantitatively and qualitatively affect the deposition of cuticular waxes on the surface of seedling leaves .
The gl1 locus has been molecularly cloned by transposon tagging with the Mutator transposon system .
The epi23 cDNA was isolated by subtractive hybridization as an epidermis-specific mRNA from Senecio odora ( Kleinia odora ) .
The deduced amino acid sequence of the GL1 and EPI23 proteins are very similar to each other and to two other plant proteins in which the sequences were deduced from their respective mRNAs .
These are the Arabidopsis CER1 protein , which is involved in cuticular wax deposition on siliques , stems , and leaves of that plant , and the protein coded by the rice expressed sequence tag RICS2751A .
All four proteins are predicted to be localized in a membrane via a common NH2-terminal domain , which consists of either five or seven membrane-spanning helices .
The COOH-terminal portion of each of these proteins , although less conserved , is predicted to be a water-soluble , globular domain .
These sequence similarities indicate that these plant orthologs may belong to a superfamily of membrane-bound receptors that have been extensively characterized from animals , including the HIV co-receptor fusin ( also termed CXCR4 ) . | Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: Mutations at the glossy1 ( gl1 ) locus of maize ( Zea mays L ) quantitatively and qualitatively affect the deposition of cuticular waxes on the surface of seedling leaves .
[ Sen. 2, subscore: 1.00 ]: The gl1 locus has been molecularly cloned by transposon tagging with the Mutator transposon system .
[ Sen. 4, subscore: 1.00 ]: The deduced amino acid sequence of the GL1 and EPI23 proteins are very similar to each other and to two other plant proteins in which the sequences were deduced from their respective mRNAs .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper ( Nilaparvata lugens ) .
| | Author: Xu XF Mei HW Luo LJ Cheng XN Li ZK .
| | Journal: Citation: V : 104 ( 2-3 ) P : 248-253 Year: 2002 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub12582694 Accession (PMID): 12582694 | | Abstract: Quantitative trait loci ( QTLs ) , conferring quantitative resistance to rice brown planthopper ( BPH ) , were investigated using 160 F ( 11 ) recombinant inbred lines ( RILs ) from the Lemont/Teqing cross , a complete RFLP map , and replicated phenotyping of seedbox inoculation .
The paternal indica parent , Teqing , was more-resistant to BPH than the maternal japonica parent , Lemont .
The RILs showed transgressive segregation for resistance to BPH .
Seven main-effect QTLs and many epistatic QTL pairs were identified and mapped on the 12 rice chromosomes .
Collectively , the main-effect and epistatic QTLs accounted for over 70% of the total variation in damage scores .
Teqing has the resistance allele at four main-effect QTLs , and the Lemont allele resulted in resistance at the other three .
Of the main-effect QTLs identified , QBphr5b was mapped to the vicinity of gl1 , a major gene controlling leaf and stem pubescence .
The Teqing allele controlling leaf and stem pubescence was associated with resistance , while the Lemont allele for glabrous stem and leaves was associated with susceptibility , indicating that this gene may have contributed to resistance through antixenosis .
Similar to the reported BPH resistance genes , the other six detected main-effect QTLs were all mapped to regions where major disease resistance genes locate , suggesting they might have contributed either to antibiosis or tolerance .
Our results indicated that marker-aided pyramiding of major resistance genes and QTLs should provide effective and stable control over this devastating pest | Matching Sentences:
[ Sen. 7, subscore: 1.00 ]: Of the main-effect QTLs identified , QBphr5b was mapped to the vicinity of gl1 , a major gene controlling leaf and stem pubescence .
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Score: 1.00 |
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| Title: Wax crystal-sparse leaf2 , a rice homologue of WAX2/GL1 , is involved in synthesis of leaf cuticular wax .
| | Author: Mao B Cheng Z Lei C Xu F Gao S Ren Y Wang J Zhang X Wang J Wu F Guo X Liu X Wu C Wang H Wan J | | Journal: Planta Citation: V : 235 P : 39-52 Year: 2012 Type: In-Data-Review | | Literature: oryza Field: abstract Doc ID: pub21809091 Accession (PMID): 21809091 | | Abstract: Epicuticular wax in plants limits non-stomatal water loss , inhibits postgenital organ fusion , protects plants against damage from UV radiation and imposes a physical barrier against pathogen infection .
Here , we give a detailed description of the genetic , physiological and morphological consequences of a mutation in the rice gene WSL2 , based on a comparison between the wild-type and an EMS mutant .
The mutants leaf cuticle membrane is thicker and less organized than that of the wild type , and its total wax content is diminished by 80% .
The mutant is also more sensitive to drought stress .
WSL2 was isolated by positional cloning , and was shown to encode a homologue of the Arabidopsis thaliana genes CER3/WAX2/YRE/FLP1 and the maize gene GL1 .
It is expressed throughout the plant , except in the root .
A transient assay carried out in both A thaliana and rice protoplasts showed that the gene product is deposited in the endoplasmic reticulum .
An analysis of the overall composition of the wax revealed that the mutant produces a substantially reduced quantity of C22-C32 fatty acids , which suggests that the function of WSL2 is associated with the elongation of very long-chain fatty acids .
| Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: WSL2 was isolated by positional cloning , and was shown to encode a homologue of the Arabidopsis thaliana genes CER3/WAX2/YRE/FLP1 and the maize gene GL1 .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
