| 7 matches found in 3 documents. Search time: 0.322 seconds. |
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| Title: The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a .
| | Author: Purwestri YA Ogaki Y Tamaki S Tsuji H Shimamoto K | | Journal: Plant Cell Physiol Citation: V : 50 P : 429-38 Year: 2009 Type: MEDLINE | | Literature: oryza Field: abstract Doc ID: pub19179350 Accession (PMID): 19179350 | | Abstract: Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis , respectively ; however , the molecular mechanisms of their function remain to be determined .
In this study , we identified GF14c ( a 14-3-3 protein ) as an Hd3a-interacting protein in a yeast two-hybrid screen .
In vitro and in vivo experiments , using a combination of pull-down assays and bimolecular fluorescence complementation , confirmed the interaction between Hd3a and GF14c .
Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering .
GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions .
These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a .
Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis , our results in rice provide important findings about FT signaling in plants .
| Matching Sentences:
[ Sen. 2, subscore: 1.00 ]: In this study , we identified GF14c ( a 14-3-3 protein ) as an Hd3a-interacting protein in a yeast two-hybrid screen .
[ Sen. 3, subscore: 1.00 ]: In vitro and in vivo experiments , using a combination of pull-down assays and bimolecular fluorescence complementation , confirmed the interaction between Hd3a and GF14c .
[ Sen. 4, subscore: 1.00 ]: Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering .
[ Sen. 5, subscore: 1.00 ]: GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions .
[ Sen. 6, subscore: 1.00 ]: These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress .
| | Author: Chen F Li Q Sun L He Z | | Journal: DNA Res .
Citation: V : 13 ( 2 ) P : 53-63 Year: 2006 Type: ARTICLE | | Literature: oryza Field: abstract Doc ID: pub16766513 Accession (PMID): 16766513 | | Abstract: 14-3-3 proteins function as major regulators of primary metabolism and cellular signal transduction in plants .
However , their involvement in plant defense and stress responses is largely unknown .
In order to better address functions of the rice 14-3-3/GF14 proteins in defense and abiotic stress responses , we examined the rice GF14 family that comprises eight numbers .
The phylogenetic comparison with the Arabidopsis 14-3-3 family revealed that the majority of rice GF14s might have evolved as an independent branch .
At least four rice GF14 genes , GF14b , GF14c , GF14e and Gf14f were differentially regulated in the interactions of rice-Magnaporthe grisea and rice-Xanthomonas oryzae pv . oryzae , and the incompatible interactions stronger induced the genes than the compatible interactions .
These GF14 genes were also induced by the defense compounds , benzothiadiazole , methyl jasmonate , ethephon and hydrogen peroxide .
Similarly , they were differentially regulated by salinity , drought , wounding and abscisic acid .
Tissue-specific analysis and expression of GF14-YFP fusions revealed that the four GF14 isoforms were expressed with it issue specificity and accumulated differentially in the cytoplasm and nucleus .
Our current study provides fundamental information for the further investigation of the rice GF14 proteins . | Matching Sentences:
[ Sen. 5, subscore: 1.00 ]: At least four rice GF14 genes , GF14b , GF14c , GF14e and Gf14f were differentially regulated in the interactions of rice-Magnaporthe grisea and rice-Xanthomonas oryzae pv . oryzae , and the incompatible interactions stronger induced the genes than the compatible interactions .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
Score: 1.00 |
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| Title: The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a .
| | Author: Purwestri YA Ogaki Y Tamaki S Tsuji H Shimamoto K | | Journal: Plant Cell Physiol Citation: V : 50 P : 429-38 Year: 2009 Type: MEDLINE | | Literature: oryza Field: title Doc ID: pub19179350 Accession (PMID): 19179350 | | Abstract: Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis , respectively ; however , the molecular mechanisms of their function remain to be determined .
In this study , we identified GF14c ( a 14-3-3 protein ) as an Hd3a-interacting protein in a yeast two-hybrid screen .
In vitro and in vivo experiments , using a combination of pull-down assays and bimolecular fluorescence complementation , confirmed the interaction between Hd3a and GF14c .
Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering .
GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions .
These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a .
Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis , our results in rice provide important findings about FT signaling in plants .
| Matching Sentences:
[ Sen. 1, subscore: 1.00 ]: The 14-3-3 protein GF14c acts as a negative regulator of flowering in rice by interacting with the florigen Hd3a .
| | Supplemental links/files: reference in endnote online text related articles pubmed citation | |
