14 matches found in 10 documents. Search time: 0.339 seconds. |
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Score: 2.00 | Title: Metabolism and Biological Activity of Gibberellin A4 in Vegetative Shoots of Zea mays , Oryza sativa , and Arabidopsis thaliana .
| Author: Kobayashi M Gaskin P Spray CR Suzuki Y Phinney BO MacMillan J | Journal: Citation: V : 102 ( 2 ) P : 379-386 Year: 1993 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub12231829 Accession (PMID): 12231829 | Abstract: [ 17-13C , 3H ] Gibberellin A4 ( GA4 ) was injected into the shoots of tall ( W23/L317 ) , dwarf-1 ( d1 ) , and dwarf-5 ( d5 ) Zea mays L ( maize ) ; tall ( cv Nipponbare ) , dwarf-x ( dx ) , and dwarf-y ( dy ) Oryza sativa L ( rice ) ; and tall ( ecotype Landsberg erecta ) , ga4 , and ga5 Arabidopsis thaliana ( L ) Heynh .
[ 13C ] GA4 and its metabolites were identified from the shoots by full-scan gas chromatography-mass spectrometry and Kovats retention indices .
GA4 was metabolized to GA1 in all nine genotypes .
GA4 was also metabolized in some of the genotypes to 3-epi-GA1 , GA2 , 2 [ beta ] -OH-GA2 , 3-epi-GA2 , endo-GA4 , 16 [ alpha ] , 17-H2-16 , 17- ( OH ) 2-GA4 , GA34 , endo-GA34 , GA58 , 15-epi-GA63 , GA71 , and 16-epi-GA82 .
No evidence was found for the metabolism of GA4 to GA7 or of GA4 to GA3 .
The bioactivities of GA4 and GA1 were determined using the six dwarf mutants for assay .
GA4 and GA1 had similar activities for the maize and rice mutants .
For the Arabidopsis mutants , GA4 was more active than GA1 at low dosages ; GA4 was less active than GA1 at higher dosages . | Matching Sentences: [ Sen. 4, subscore: 1.00 ]: GA4 was also metabolized in some of the genotypes to 3-epi-GA1 , GA2 , 2 [ beta ] -OH-GA2 , 3-epi-GA2 , endo-GA4 , 16 [ alpha ] , 17-H2-16 , 17- ( OH ) 2-GA4 , GA34 , endo-GA34 , GA58 , 15-epi-GA63 , GA71 , and 16-epi-GA82 . [ Sen. 5, subscore: 1.00 ]: No evidence was found for the metabolism of GA4 to GA7 or of GA4 to GA3 .
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Score: 2.00 | Title: Isolation of a gibberellin-producing fungus ( Penicillium sp .
MH7 ) and growth promotion of Crown daisy ( Chrysanthemum coronarium ) .
| Author: Hamayun M Khan SA Iqbal I Ahmad B Lee IJ | Journal: J Microbiol Biotechnol Citation: V : 20 P : 202-7 Year: 2010 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub20134253 Accession (PMID): 20134253 | Abstract: Plant growth promoting fungi ( PGPF ) are well known for the production of useful secondary metabolites .
However , limited informations are available on gibberellin ( GA ) production capacity of PGPF of endophytic origin .
In current study , 15 fungal endophytes were isolated from the roots of Crown daisy , and then screened on Waito-c rice , in order to identify plant growth promoting fungi .
The fungal isolate MH7 significantly increased shoot length ( 12 . 1 cm ) of Waito-c in comparison to control treatment ( 7 . 9 cm ) .
In a separate experiment , culture filtrate ( CF ) of MH7 significantly promoted growth attributes of Crown daisy .
The MH7 CF was analyzed for gibberellins and it contained all physiologically active gibberellins ( GA1 , 1 . 37 ng/ml ; GA3 , 5 . 88 ng/ml ; GA4 , 8 . 62 ng/ml and GA7 , 2 . 05 ng/ml ) in conjunction with physiologically inactive GA9 ( 0 . 83 ng/ml ) , GA12 ( 0 . 44 ng/ml ) , GA15 ( 0 . 74 ng/ml ) , GA19 ( 1 . 16 ng/ml ) and GA20 ( 0 . 98 ng/ml ) .
The CF of MH7 produced higher amounts of GA3 , GA4 , GA7 , GA9 and GA12 than wild type Fusarium fujikuroi , which was used as control for GA production .
The fungal isolate MH7 was later identified as a new strain of Penicillium on the basis of morphological characteristics and phylogenetic analysis of 18S rDNA sequence .
| Matching Sentences: [ Sen. 6, subscore: 1.00 ]: The MH7 CF was analyzed for gibberellins and it contained all physiologically active gibberellins ( GA1 , 1 . 37 ng/ml ; GA3 , 5 . 88 ng/ml ; GA4 , 8 . 62 ng/ml and GA7 , 2 . 05 ng/ml ) in conjunction with physiologically inactive GA9 ( 0 . 83 ng/ml ) , GA12 ( 0 . 44 ng/ml ) , GA15 ( 0 . 74 ng/ml ) , GA19 ( 1 . 16 ng/ml ) and GA20 ( 0 . 98 ng/ml ) . [ Sen. 7, subscore: 1.00 ]: The CF of MH7 produced higher amounts of GA3 , GA4 , GA7 , GA9 and GA12 than wild type Fusarium fujikuroi , which was used as control for GA production .
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Score: 2.00 | Title: Gibberellin production and plant growth promotion from pure cultures of Cladosporium sp .
MH-6 isolated from cucumber ( Cucumis sativus L ) .
| Author: Hamayun M Khan SA Khan AL Rehman G Kim YH Iqbal I Hussain J Sohn EY Lee IJ | Journal: Mycologia Citation: V : 102 P : 989-95 Year: 2010 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub20943499 Accession (PMID): 20943499 | Abstract: Gibberellin ( GA ) production by soil fungi has received little attention , although substantial work has been carried out on other aspects of plant growth promoting fungi ( PGPF ) .
In our studies we investigated GA production and growth-promoting capacity of a novel fungal strain isolated from the roots of soil-grown cucumber .
Pure cultures of 19 endophytic fungi were tested for shoot length promotion of Waito-C rice to identify the GA production capacity of these fungal isolates .
Isolate MH-6 significantly increased shoot length ( 12 . 9 cm ) of Waito-C , in comparison to control treatments .
Bioassay with culture filtrate ( CF ) of MH-6 also significantly promoted growth attributes of cucumber plants .
Analysis of MH-6 CF showed the presence of physiologically active ( GA1 , 1 . 97 ng/mL ; GA3 , 5 . 18 ng/mL ; GA4 , 13 . 35 ng/mL and GA7 , 2 . 4 ng/ mL ) in conjunction with physiologically inactive ( GA9 [ 0 . 69 ng/mL ] , GA12 [ 0 . 24 ng/mL ] , GA15 [ 0 . 68 ng/ mL , GA19 [ 1 . 94 ng/mL and GA20 [ 0 . 78 ng/mL ] ) gibberellins .
The CF of MH-6 produced greater amounts of GA3 , GA4 , GA7 and GA19 than wild type Fusarium fujikuroi , a fungus known for high production of GA .
The fungal isolate MH-6 was identified as a new strain of Cladosporium sp . on the basis of sequence homology ( 99% ) and phylogenetic analysis of 18S rDNA sequence .
| Matching Sentences: [ Sen. 6, subscore: 1.00 ]: Analysis of MH-6 CF showed the presence of physiologically active ( GA1 , 1 . 97 ng/mL ; GA3 , 5 . 18 ng/mL ; GA4 , 13 . 35 ng/mL and GA7 , 2 . 4 ng/ mL ) in conjunction with physiologically inactive ( GA9 [ 0 . 69 ng/mL ] , GA12 [ 0 . 24 ng/mL ] , GA15 [ 0 . 68 ng/ mL , GA19 [ 1 . 94 ng/mL and GA20 [ 0 . 78 ng/mL ] ) gibberellins . [ Sen. 7, subscore: 1.00 ]: The CF of MH-6 produced greater amounts of GA3 , GA4 , GA7 and GA19 than wild type Fusarium fujikuroi , a fungus known for high production of GA .
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Score: 2.00 | Title: Gibberellin-producing endophytic fungi isolated from Monochoria vaginalis .
| Author: Ahmad N Hamayun M Khan SA Khan AL Lee IJ Shin DH | Journal: J Microbiol Biotechnol Citation: V : 20 P : 1744-9 Year: 2010 Type: MEDLINE | Literature: oryza Field: abstract Doc ID: pub21193832 Accession (PMID): 21193832 | Abstract: The role of endophytic fungi in plant growth and development is well documented .
However , endophytic fungi with growth promotion capacity have never been isolated from weeds previously .
In the current study , we isolated 8 fungal endophytes from the roots of Monochoria vaginalis , a serious weed of rice paddy in Korea .
These isolates were screened on Waito-C , in order to identify plant growth promoting metabolites .
Two fungal isolates ( M5 . A & M1 . 5 ) significantly promoted the plant height and shoot length of Waito-C during preliminary screening experiments .
The culture filtrates ( CFs ) of M5 . A and M1 . 5 also promoted the shoot length of Echinocloa crusgalli .
Gibberellins ( GAs ) analysis of the CFs of M5 . A and M1 . 5 showed that these endophytic fungi secrete higher quantities of GAs as compared with wild-type G fujikuroi KCCM12329 .
The CF of M5 . A contained bioactive GAs ( GA3 , 2 . 8 ng/ml ; GA4 , 2 . 6 ng/ml , and GA7 , 6 . 68 ng/ml ) in conjunction with physiologically inactive GA9 ( 1 . 61 ng/ml ) and GA24 ( 0 . 18 ng/ml ) .
The CF of M1 . 5 contained physiologically active GAs ( GA3 , 1 . 64 ng/ml ; GA4 , 1 . 37 ng/ml and GA7 , 6 . 29 ng/ml ) in conjunction with physiologically inactive GA9 ( 3 . 44 ng/ml ) , GA12 ( 0 . 3 ng/ml ) , and GA24 ( 0 . 59 ng/ml ) .
M5 . A and M1 . 5 were identified as new strains of Penicillium sp . and Aspergillus sp . , respectively , based on their 18S rDNA sequence homology and phylogenetic analysis .
| Matching Sentences: [ Sen. 8, subscore: 1.00 ]: The CF of M5 . A contained bioactive GAs ( GA3 , 2 . 8 ng/ml ; GA4 , 2 . 6 ng/ml , and GA7 , 6 . 68 ng/ml ) in conjunction with physiologically inactive GA9 ( 1 . 61 ng/ml ) and GA24 ( 0 . 18 ng/ml ) . [ Sen. 9, subscore: 1.00 ]: The CF of M1 . 5 contained physiologically active GAs ( GA3 , 1 . 64 ng/ml ; GA4 , 1 . 37 ng/ml and GA7 , 6 . 29 ng/ml ) in conjunction with physiologically inactive GA9 ( 3 . 44 ng/ml ) , GA12 ( 0 . 3 ng/ml ) , and GA24 ( 0 . 59 ng/ml ) .
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Score: 1.00 | Title: Biosynthesis of gibberellins in Gibberella fujikuroi : biomolecular aspects .
| Author: Tudzynski B | Journal: Appl . Microbiol . Biotechnol . Citation: V : 52 ( 3 ) P : 298-310 Year: 1999 Type: ARTICLE | Literature: oryza Field: abstract Doc ID: pub10531641 Accession (PMID): 10531641 | Abstract: Gibberellins ( GAs ) are a large family of isoprenoid plant hormones hormones , some of which are bioactive growth regulators , controlling seed germination , stem elongation , and flowering .
The rice pathogen Gibberella fujikuroi ( mating population C ) is able to produce large amounts of GAs , especially the bioactive compounds gibberellic acid ( GA3 ) and its precursors , GA4 and GA7 .
The main steps of the biosynthetic pathway have long been established from the identification of intermediates in wild-type G fujikuroi and mutant strains .
However , the genetics of the fungus have been rather under-developed , and molecular genetic studies of the GA pathway started just recently .
The progress in researching GA biosynthesis in the last 2 years resulted primarily from development of the molecular tools , eg transformation systems for the fungus , and cloning the genes encoding GA biosynthesis enzymes , such as the bifunctional ent-copalyl diphosphate/kaurene synthase and several cytochrome P450 monooxygenases .
The availability of these genes opened new horizons both for detailed study of the pathway and the regulation mechanisms at the molecular level , and for modern strain improvement programs .
This review gives a short overview of the well-known physiological and biochemical studies and concentrates mainly on the new molecular genetic data from GA research , including new information on the regulation of GA biosynthesis . | Matching Sentences: [ Sen. 2, subscore: 1.00 ]: The rice pathogen Gibberella fujikuroi ( mating population C ) is able to produce large amounts of GAs , especially the bioactive compounds gibberellic acid ( GA3 ) and its precursors , GA4 and GA7 .
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